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Effect of dietary supplementation with increasing doses of docosahexaenoic acid on neutrophil lipid composition and leukotriene production in human healthy volunteers.
Br J Nutr. 2008 Oct; 100(4):829-33.BJ

Abstract

n-3 PUFA supplementation helps in the prevention or treatment of inflammatory diseases and CVD. However, many supplementations reported sofar are either a combination of n-3 PUFA or used large daily amounts of n-3 PUFA dosages. The present study investigated the influence of increasing dose intake of DHA on the fatty acid composition of phospholipids in neutrophils and on their capability to produce leukotrienes(LT) B4 and B5 in vitro. Twelve healthy volunteers were supplemented with increasing daily doses of DHA (200, 400, 800 and 1600 mg, each dose in TAG containing DHA as the only PUFA and for a 2-week period). At the end of each supplementation period, neutrophil fatty acid composition,and LTB4 and LTB5 production were determined by GC and liquid chromatography-tandem MS, respectively. The DHA/arachidonic acid ratio increased in a dose-dependent manner with respect to the increasing doses of DHA supplementation and was significantly different from baseline after supplementation with either 400, 800 or 1600 mg DHA. The LTB5/LTB4 ratio was significantly increased compared to baseline after supplementation with 800 and 1600 mg DHA. LTB5/LTB4 and DHA/arachidonic acid ratios were correlated (r 0.531, P<0.0001). The present data suggest that both changes in neutrophil lipid composition and LT production occurred with daily supplementation with 800 and 1600 mg DHA. The clinical benefits associated with these doses of DHA in inflammatory diseases remain to be investigated.

Authors+Show Affiliations

Université de Lyon, INSERM UMR 870, INSA-Lyon, RMND, F-69621, INRA UMR 1235, Université Lyon 1, Hospices Civils de Lyon, France. FStanke@chu-grenoble.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18304388

Citation

Stanke-Labesque, Françoise, et al. "Effect of Dietary Supplementation With Increasing Doses of Docosahexaenoic Acid On Neutrophil Lipid Composition and Leukotriene Production in Human Healthy Volunteers." The British Journal of Nutrition, vol. 100, no. 4, 2008, pp. 829-33.
Stanke-Labesque F, Molière P, Bessard J, et al. Effect of dietary supplementation with increasing doses of docosahexaenoic acid on neutrophil lipid composition and leukotriene production in human healthy volunteers. Br J Nutr. 2008;100(4):829-33.
Stanke-Labesque, F., Molière, P., Bessard, J., Laville, M., Véricel, E., & Lagarde, M. (2008). Effect of dietary supplementation with increasing doses of docosahexaenoic acid on neutrophil lipid composition and leukotriene production in human healthy volunteers. The British Journal of Nutrition, 100(4), 829-33. https://doi.org/10.1017/S0007114508923692
Stanke-Labesque F, et al. Effect of Dietary Supplementation With Increasing Doses of Docosahexaenoic Acid On Neutrophil Lipid Composition and Leukotriene Production in Human Healthy Volunteers. Br J Nutr. 2008;100(4):829-33. PubMed PMID: 18304388.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of dietary supplementation with increasing doses of docosahexaenoic acid on neutrophil lipid composition and leukotriene production in human healthy volunteers. AU - Stanke-Labesque,Françoise, AU - Molière,Patrick, AU - Bessard,Jeanine, AU - Laville,Martine, AU - Véricel,Evelyne, AU - Lagarde,Michel, Y1 - 2008/02/28/ PY - 2008/2/29/pubmed PY - 2009/5/14/medline PY - 2008/2/29/entrez SP - 829 EP - 33 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 100 IS - 4 N2 - n-3 PUFA supplementation helps in the prevention or treatment of inflammatory diseases and CVD. However, many supplementations reported sofar are either a combination of n-3 PUFA or used large daily amounts of n-3 PUFA dosages. The present study investigated the influence of increasing dose intake of DHA on the fatty acid composition of phospholipids in neutrophils and on their capability to produce leukotrienes(LT) B4 and B5 in vitro. Twelve healthy volunteers were supplemented with increasing daily doses of DHA (200, 400, 800 and 1600 mg, each dose in TAG containing DHA as the only PUFA and for a 2-week period). At the end of each supplementation period, neutrophil fatty acid composition,and LTB4 and LTB5 production were determined by GC and liquid chromatography-tandem MS, respectively. The DHA/arachidonic acid ratio increased in a dose-dependent manner with respect to the increasing doses of DHA supplementation and was significantly different from baseline after supplementation with either 400, 800 or 1600 mg DHA. The LTB5/LTB4 ratio was significantly increased compared to baseline after supplementation with 800 and 1600 mg DHA. LTB5/LTB4 and DHA/arachidonic acid ratios were correlated (r 0.531, P<0.0001). The present data suggest that both changes in neutrophil lipid composition and LT production occurred with daily supplementation with 800 and 1600 mg DHA. The clinical benefits associated with these doses of DHA in inflammatory diseases remain to be investigated. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/18304388/Effect_of_dietary_supplementation_with_increasing_doses_of_docosahexaenoic_acid_on_neutrophil_lipid_composition_and_leukotriene_production_in_human_healthy_volunteers_ L2 - https://www.cambridge.org/core/product/identifier/S0007114508923692/type/journal_article DB - PRIME DP - Unbound Medicine ER -