Tags

Type your tag names separated by a space and hit enter

A polysaccharide from Lichina pygmaea and L. confinis supports the recognition of Lichinomycetes.
Mycol Res. 2008 Mar; 112(Pt 3):381-8.MR

Abstract

The lichen-forming order Lichinales, generally characterized by prototunicate asci and the development of thalli with cyanobacteria, has recently been recognized as a separate class of ascomycetes, Lichinomycetes, as a result of molecular phylogenetic studies. As alkali and water-soluble (F1SS) polysaccharides reflect phylogeny in other ascomycetes, a polysaccharide from Lichina pygmaea and L. confinis was purified and characterized to investigate whether these F1SS compounds in the Lichinomycetes were distinctive. Nuclear magnetic resonance (NMR) spectroscopy and chemical analyses revealed this as a galactomannan comprising a repeating unit consisting of an alpha-(1-->6)-mannan backbone, mainly substituted by single alpha-galactofuranose residues at the O-2- or the O-2,4- positions linked to a small mannan core. With the exception of the trisubstituted mannopyranose residues previously described in polysaccharides from other lichens belonging to orders now placed in Lecanoromycetes, the structure of this galactomannan most closely resembles those found in several members of the Onygenales in Eurotiomycetes. Our polysaccharide data support molecular studies showing that Lichina species are remote from Lecanoromycetes as the galactofuranose residues are in the alpha-configuration. That the Lichinomycetes were part of an ancestral lichenized group can not be established from the present data because the extracted polysaccharide does not have the galactofuranose residue in the beta configuration; however, the data does suggest that an ancestor of the Lichinomycetes contained a mannan and was part of an early radiation in the ascomycetes.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Prieto A
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, ES-28040 Madrid, Spain. aliprieto@cib.csic.es
Leal JA
No affiliation info available
Bernabé M
No affiliation info available
Hawksworth DL
No affiliation info available

MeSH

AscomycotaHydrolysisLichensMagnetic Resonance SpectroscopyMethylationPolysaccharides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18304792

Citation

Prieto, Alicia, et al. "A Polysaccharide From Lichina Pygmaea and L. Confinis Supports the Recognition of Lichinomycetes." Mycological Research, vol. 112, no. Pt 3, 2008, pp. 381-8.
Prieto A, Leal JA, Bernabé M, et al. A polysaccharide from Lichina pygmaea and L. confinis supports the recognition of Lichinomycetes. Mycol Res. 2008;112(Pt 3):381-8.
Prieto, A., Leal, J. A., Bernabé, M., & Hawksworth, D. L. (2008). A polysaccharide from Lichina pygmaea and L. confinis supports the recognition of Lichinomycetes. Mycological Research, 112(Pt 3), 381-8. https://doi.org/10.1016/j.mycres.2007.10.013
Prieto A, et al. A Polysaccharide From Lichina Pygmaea and L. Confinis Supports the Recognition of Lichinomycetes. Mycol Res. 2008;112(Pt 3):381-8. PubMed PMID: 18304792.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A polysaccharide from Lichina pygmaea and L. confinis supports the recognition of Lichinomycetes. AU - Prieto,Alicia, AU - Leal,J Antonio, AU - Bernabé,Manuel, AU - Hawksworth,David L, Y1 - 2007/11/07/ PY - 2006/10/05/received PY - 2007/09/21/revised PY - 2007/10/25/accepted PY - 2008/2/29/pubmed PY - 2008/6/18/medline PY - 2008/2/29/entrez SP - 381 EP - 8 JF - Mycological research JO - Mycol Res VL - 112 IS - Pt 3 N2 - The lichen-forming order Lichinales, generally characterized by prototunicate asci and the development of thalli with cyanobacteria, has recently been recognized as a separate class of ascomycetes, Lichinomycetes, as a result of molecular phylogenetic studies. As alkali and water-soluble (F1SS) polysaccharides reflect phylogeny in other ascomycetes, a polysaccharide from Lichina pygmaea and L. confinis was purified and characterized to investigate whether these F1SS compounds in the Lichinomycetes were distinctive. Nuclear magnetic resonance (NMR) spectroscopy and chemical analyses revealed this as a galactomannan comprising a repeating unit consisting of an alpha-(1-->6)-mannan backbone, mainly substituted by single alpha-galactofuranose residues at the O-2- or the O-2,4- positions linked to a small mannan core. With the exception of the trisubstituted mannopyranose residues previously described in polysaccharides from other lichens belonging to orders now placed in Lecanoromycetes, the structure of this galactomannan most closely resembles those found in several members of the Onygenales in Eurotiomycetes. Our polysaccharide data support molecular studies showing that Lichina species are remote from Lecanoromycetes as the galactofuranose residues are in the alpha-configuration. That the Lichinomycetes were part of an ancestral lichenized group can not be established from the present data because the extracted polysaccharide does not have the galactofuranose residue in the beta configuration; however, the data does suggest that an ancestor of the Lichinomycetes contained a mannan and was part of an early radiation in the ascomycetes. SN - 0953-7562 UR - https://www.unboundmedicine.com/medline/citation/18304792/A_polysaccharide_from_Lichina_pygmaea_and_L__confinis_supports_the_recognition_of_Lichinomycetes_ DB - PRIME DP - Unbound Medicine ER -