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Ultra sensitive sensor with enhanced dynamic range for high speed detection of multi-color fluorescence radiation.
Biosens Bioelectron. 2008 May 15; 23(10):1512-8.BB

Abstract

This paper describes design of the new ultra sensitive sensor system for fluorescence detection applications. System comprises two units: optical spectra separation unit and detection unit. Optical unit of the sensor performs spatial spectra separation of signal from the laser excited fluorescence, and resulting spectra is collected in the detection part of the system. Optical part is built using diffraction grating as spectra separation element. Detection part comprises 32-channel photomultiplier tube working in single photon counting mode with our 32-channel amplifier. Using single photon detection technique and specific signal processing algorithms for collected data, the proposed system allows to achieve unique combination of characteristics--high sensitivity, high detection speed and wide linearity dynamic range comparing to existing commercial instruments. DNA sequencing experiments with new sensor as detection device, and using two types of lasers (Ar-ion and Nd-YAG) were carried out, yielding sequencing traces which have quality factor of 20 for read lengths as long as 650 base pairs.

Authors+Show Affiliations

Department of Electrical and Computer Engineering, Stony Brook University, Stony Brook, NY 11794-2350, USA. atsupryk@ic.sunysb.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

18304800

Citation

Tsupryk, A, et al. "Ultra Sensitive Sensor With Enhanced Dynamic Range for High Speed Detection of Multi-color Fluorescence Radiation." Biosensors & Bioelectronics, vol. 23, no. 10, 2008, pp. 1512-8.
Tsupryk A, Tovkach I, Gavrilov D, et al. Ultra sensitive sensor with enhanced dynamic range for high speed detection of multi-color fluorescence radiation. Biosens Bioelectron. 2008;23(10):1512-8.
Tsupryk, A., Tovkach, I., Gavrilov, D., Kosobokova, O., Gudkov, G., Tyshko, G., Gorbovitski, B., & Gorfinkel, V. (2008). Ultra sensitive sensor with enhanced dynamic range for high speed detection of multi-color fluorescence radiation. Biosensors & Bioelectronics, 23(10), 1512-8. https://doi.org/10.1016/j.bios.2008.01.015
Tsupryk A, et al. Ultra Sensitive Sensor With Enhanced Dynamic Range for High Speed Detection of Multi-color Fluorescence Radiation. Biosens Bioelectron. 2008 May 15;23(10):1512-8. PubMed PMID: 18304800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultra sensitive sensor with enhanced dynamic range for high speed detection of multi-color fluorescence radiation. AU - Tsupryk,A, AU - Tovkach,I, AU - Gavrilov,D, AU - Kosobokova,O, AU - Gudkov,G, AU - Tyshko,G, AU - Gorbovitski,B, AU - Gorfinkel,V, Y1 - 2008/01/19/ PY - 2007/10/29/received PY - 2007/12/16/revised PY - 2008/01/03/accepted PY - 2008/2/29/pubmed PY - 2008/8/5/medline PY - 2008/2/29/entrez SP - 1512 EP - 8 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 23 IS - 10 N2 - This paper describes design of the new ultra sensitive sensor system for fluorescence detection applications. System comprises two units: optical spectra separation unit and detection unit. Optical unit of the sensor performs spatial spectra separation of signal from the laser excited fluorescence, and resulting spectra is collected in the detection part of the system. Optical part is built using diffraction grating as spectra separation element. Detection part comprises 32-channel photomultiplier tube working in single photon counting mode with our 32-channel amplifier. Using single photon detection technique and specific signal processing algorithms for collected data, the proposed system allows to achieve unique combination of characteristics--high sensitivity, high detection speed and wide linearity dynamic range comparing to existing commercial instruments. DNA sequencing experiments with new sensor as detection device, and using two types of lasers (Ar-ion and Nd-YAG) were carried out, yielding sequencing traces which have quality factor of 20 for read lengths as long as 650 base pairs. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/18304800/Ultra_sensitive_sensor_with_enhanced_dynamic_range_for_high_speed_detection_of_multi_color_fluorescence_radiation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00027-4 DB - PRIME DP - Unbound Medicine ER -