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First outbreak of multidrug-resistant Klebsiella pneumoniae producing both SHV-12-type extended-spectrum beta-lactamase and DHA-1-type AmpC beta-lactamase at a Korean hospital.
Yonsei Med J. 2008 Feb 29; 49(1):53-7.YM

Abstract

PURPOSE

Coexistence of different classes of beta-lactamases in a single bacterial isolate may pose diagnostic and therapeutic challenges. We investigated a spread of Klebsiella pneumoniae isolates co-producing an AmpC beta-lactamase and an extended-spectrum beta-lactamase (ESBL) in a university hospital.

MATERIALS AND METHODS

Over a three-month period, a total of 11 K. pneumoniae isolates, which exhibited resistance to cefotaxime, aztreonam, and cefoxitin, were isolated. These isolates showed positive to ESBLs by double disk tests. Minimal inhibitory concentrations (MICs) were determined by broth microdilution testing. All isolates were examined by isoelectric focusing, PCR and sequence analysis to identify bla(SHV) and bla(DHA), and molecular typing by pulsed-field gel electrophoresis (PFGE).

RESULTS

All 11 isolates were highly resistant (MIC, > or = 128microg/ml) to ceftazidime, aztreonam, and cefoxitin, while they were susceptible (MIC, < or = 2microg/ml) to imipenem. The bla(SHV-12) and bla(DHA-1) genes were detected by PCR and sequence analysis. PFGE revealed a similar pattern in 10 of the 11 strains tested.

CONCLUSION

This is the first outbreak report of K. pneumoniae in Korea which co-produced SHV-12 and DHA-1 beta-lactamase, and we suggest a clonal spread of multidrug-resistant K. pneumoniae at a hospital.

Authors+Show Affiliations

Department of Laboratory Medicine, Korean University Medical College, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18306469

Citation

Roh, Kyoung Ho, et al. "First Outbreak of Multidrug-resistant Klebsiella Pneumoniae Producing Both SHV-12-type Extended-spectrum Beta-lactamase and DHA-1-type AmpC Beta-lactamase at a Korean Hospital." Yonsei Medical Journal, vol. 49, no. 1, 2008, pp. 53-7.
Roh KH, Uh Y, Kim JS, et al. First outbreak of multidrug-resistant Klebsiella pneumoniae producing both SHV-12-type extended-spectrum beta-lactamase and DHA-1-type AmpC beta-lactamase at a Korean hospital. Yonsei Med J. 2008;49(1):53-7.
Roh, K. H., Uh, Y., Kim, J. S., Kim, H. S., Shin, D. H., & Song, W. (2008). First outbreak of multidrug-resistant Klebsiella pneumoniae producing both SHV-12-type extended-spectrum beta-lactamase and DHA-1-type AmpC beta-lactamase at a Korean hospital. Yonsei Medical Journal, 49(1), 53-7. https://doi.org/10.3349/ymj.2008.49.1.53
Roh KH, et al. First Outbreak of Multidrug-resistant Klebsiella Pneumoniae Producing Both SHV-12-type Extended-spectrum Beta-lactamase and DHA-1-type AmpC Beta-lactamase at a Korean Hospital. Yonsei Med J. 2008 Feb 29;49(1):53-7. PubMed PMID: 18306469.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - First outbreak of multidrug-resistant Klebsiella pneumoniae producing both SHV-12-type extended-spectrum beta-lactamase and DHA-1-type AmpC beta-lactamase at a Korean hospital. AU - Roh,Kyoung Ho, AU - Uh,Young, AU - Kim,Jae-Seok, AU - Kim,Han-Sung, AU - Shin,Dong Hoon, AU - Song,Wonkeun, PY - 2008/2/29/pubmed PY - 2008/5/31/medline PY - 2008/2/29/entrez SP - 53 EP - 7 JF - Yonsei medical journal JO - Yonsei Med J VL - 49 IS - 1 N2 - PURPOSE: Coexistence of different classes of beta-lactamases in a single bacterial isolate may pose diagnostic and therapeutic challenges. We investigated a spread of Klebsiella pneumoniae isolates co-producing an AmpC beta-lactamase and an extended-spectrum beta-lactamase (ESBL) in a university hospital. MATERIALS AND METHODS: Over a three-month period, a total of 11 K. pneumoniae isolates, which exhibited resistance to cefotaxime, aztreonam, and cefoxitin, were isolated. These isolates showed positive to ESBLs by double disk tests. Minimal inhibitory concentrations (MICs) were determined by broth microdilution testing. All isolates were examined by isoelectric focusing, PCR and sequence analysis to identify bla(SHV) and bla(DHA), and molecular typing by pulsed-field gel electrophoresis (PFGE). RESULTS: All 11 isolates were highly resistant (MIC, > or = 128microg/ml) to ceftazidime, aztreonam, and cefoxitin, while they were susceptible (MIC, < or = 2microg/ml) to imipenem. The bla(SHV-12) and bla(DHA-1) genes were detected by PCR and sequence analysis. PFGE revealed a similar pattern in 10 of the 11 strains tested. CONCLUSION: This is the first outbreak report of K. pneumoniae in Korea which co-produced SHV-12 and DHA-1 beta-lactamase, and we suggest a clonal spread of multidrug-resistant K. pneumoniae at a hospital. SN - 0513-5796 UR - https://www.unboundmedicine.com/medline/citation/18306469/First_outbreak_of_multidrug_resistant_Klebsiella_pneumoniae_producing_both_SHV_12_type_extended_spectrum_beta_lactamase_and_DHA_1_type_AmpC_beta_lactamase_at_a_Korean_hospital_ L2 - https://www.eymj.org/DOIx.php?id=10.3349/ymj.2008.49.1.53 DB - PRIME DP - Unbound Medicine ER -