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Helicobacter pylori cagA, vacA and iceA genotypes in western Indian population of Maharashtra with varied gastroduodenal diseases.
Indian J Pathol Microbiol. 2007 Oct; 50(4):740-8.IJ

Abstract

Scarce reports relying on rapid urease test, serology and histopathology are currently known for H. pylori from Western India, Maharashtra. We investigated H. pylori genotypes at molecular level in gastro-duodenal disease population during the years 2002-2005. H. pylori presence was scored by polymerase chain reaction in the infected biopsies (n = 95) in various gastric diseases. H. pylori specific 16S rDNA gene amplification based preliminary identification coupled with protein coding gene amplification scores were assessed for the incidence. H. pylori 16S rDNA and 7 housekeeping genes were detected in all biopsies, whereas 71.18% and 28% found to be cagA positive and negative respectively. The vacA toxigenic alleles (vacA s1) and middle region subunit vac m1a were found in 54%, and 59% patients. However, the iceA1 was present in 40.06%; the iceA2 was less i.e. in 13.5% patients. The most common allelic combinations in different age groups irrespective of disease types were 13-30, 31-45, 46-60 and 61-73 were cagA-vac m1a-vacA s1-iceA1. In our analysis, PCR was found to be 100% accurate in detecting H. pylori in gastric biopsies. Among West Indian population H. pylori was found to be present, irrespective of any correlation with the genotype and gender of patients with the clinical outcome. However, the genotype incidences were related to age of the patients, wherein the age group ranging from 46 to 60 years was found be susceptible for H. pylori infection.

Authors+Show Affiliations

Molecular Biology Unit, National Centre for Cell Science, Pune.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18306540

Citation

Dharne, Mahesh Shantappa, et al. "Helicobacter Pylori cagA, vacA and iceA Genotypes in Western Indian Population of Maharashtra With Varied Gastroduodenal Diseases." Indian Journal of Pathology & Microbiology, vol. 50, no. 4, 2007, pp. 740-8.
Dharne MS, Munot H, Pujari R, et al. Helicobacter pylori cagA, vacA and iceA genotypes in western Indian population of Maharashtra with varied gastroduodenal diseases. Indian J Pathol Microbiol. 2007;50(4):740-8.
Dharne, M. S., Munot, H., Pujari, R., Kakrani, A. L., Patole, M. S., & Shouche, Y. S. (2007). Helicobacter pylori cagA, vacA and iceA genotypes in western Indian population of Maharashtra with varied gastroduodenal diseases. Indian Journal of Pathology & Microbiology, 50(4), 740-8.
Dharne MS, et al. Helicobacter Pylori cagA, vacA and iceA Genotypes in Western Indian Population of Maharashtra With Varied Gastroduodenal Diseases. Indian J Pathol Microbiol. 2007;50(4):740-8. PubMed PMID: 18306540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Helicobacter pylori cagA, vacA and iceA genotypes in western Indian population of Maharashtra with varied gastroduodenal diseases. AU - Dharne,Mahesh Shantappa, AU - Munot,Hitendra, AU - Pujari,Rajesh, AU - Kakrani,Arjun Lal, AU - Patole,Milind Shivajirao, AU - Shouche,Yogesh Shripad, PY - 2008/3/1/pubmed PY - 2008/3/26/medline PY - 2008/3/1/entrez SP - 740 EP - 8 JF - Indian journal of pathology & microbiology JO - Indian J Pathol Microbiol VL - 50 IS - 4 N2 - Scarce reports relying on rapid urease test, serology and histopathology are currently known for H. pylori from Western India, Maharashtra. We investigated H. pylori genotypes at molecular level in gastro-duodenal disease population during the years 2002-2005. H. pylori presence was scored by polymerase chain reaction in the infected biopsies (n = 95) in various gastric diseases. H. pylori specific 16S rDNA gene amplification based preliminary identification coupled with protein coding gene amplification scores were assessed for the incidence. H. pylori 16S rDNA and 7 housekeeping genes were detected in all biopsies, whereas 71.18% and 28% found to be cagA positive and negative respectively. The vacA toxigenic alleles (vacA s1) and middle region subunit vac m1a were found in 54%, and 59% patients. However, the iceA1 was present in 40.06%; the iceA2 was less i.e. in 13.5% patients. The most common allelic combinations in different age groups irrespective of disease types were 13-30, 31-45, 46-60 and 61-73 were cagA-vac m1a-vacA s1-iceA1. In our analysis, PCR was found to be 100% accurate in detecting H. pylori in gastric biopsies. Among West Indian population H. pylori was found to be present, irrespective of any correlation with the genotype and gender of patients with the clinical outcome. However, the genotype incidences were related to age of the patients, wherein the age group ranging from 46 to 60 years was found be susceptible for H. pylori infection. SN - 0377-4929 UR - https://www.unboundmedicine.com/medline/citation/18306540/Helicobacter_pylori_cagA_vacA_and_iceA_genotypes_in_western_Indian_population_of_Maharashtra_with_varied_gastroduodenal_diseases_ L2 - https://medlineplus.gov/helicobacterpyloriinfections.html DB - PRIME DP - Unbound Medicine ER -