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Inhibition of spontaneous neurotransmission in the nucleus of solitary tract of the rat by the cannabinoid agonist WIN 55212-2 is not via CB1 or CB2 receptors.

Abstract

Cannabinoids have been shown to modulate central autonomic regulation and baroreflex control of blood pressure. Both CB1 and CB2 cannabinoid receptors have been described in the nucleus tractus solitarius (NTS), which receives direct afferent projections of cardiovascular reflexes. In the present study we evaluated the effects of WIN 55212-2 (WIN), a cannabinoid agonist, on fast neurotransmission in the NTS. We recorded spontaneous post-synaptic currents using the whole-cell configuration in NTS cells in brainstem slices from young rats (25-30 days old). Application of 5 microM WIN inhibited the frequency of both glutamatergic and GABAergic sPSCs, without affecting their amplitudes. Effects of WIN were not blocked by application of the CB1 antagonist AM251, the CB2 antagonist AM630 or the vanniloid receptor TRPV1 antagonist AMG9810, suggesting that the effect of WIN is via a non-CB1 non-CB2 receptor. Neither the CB1/CB2 agonist HU210 nor the CB1 agonist ACPA affected the frequency of sPSCs. We conclude WIN inhibits the neurotransmission in the NTS of young rats via a receptor distinct from CB1 or CB2.

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  • Authors+Show Affiliations

    ,

    Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900 14049-900 Ribeirão Preto, SP, Brazil.

    , , ,

    Source

    Brain research 1200: 2008 Mar 20 pg 1-9

    MeSH

    Animals
    Benzoxazines
    Cannabinoid Receptor Agonists
    Cannabinoid Receptor Antagonists
    Cannabinoid Receptor Modulators
    Cannabinoids
    Glutamic Acid
    Inhibitory Postsynaptic Potentials
    Male
    Morpholines
    Naphthalenes
    Neural Inhibition
    Organ Culture Techniques
    Patch-Clamp Techniques
    Rats
    Rats, Wistar
    Receptor, Cannabinoid, CB1
    Receptor, Cannabinoid, CB2
    Receptors, Cannabinoid
    Solitary Nucleus
    Synaptic Transmission
    Visceral Afferents
    gamma-Aminobutyric Acid

    Pub Type(s)

    Journal Article
    Research Support, N.I.H., Extramural
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    18308297

    Citation

    Accorsi-Mendonça, Daniela, et al. "Inhibition of Spontaneous Neurotransmission in the Nucleus of Solitary Tract of the Rat By the Cannabinoid Agonist WIN 55212-2 Is Not Via CB1 or CB2 Receptors." Brain Research, vol. 1200, 2008, pp. 1-9.
    Accorsi-Mendonça D, Almado CE, Dagostin AL, et al. Inhibition of spontaneous neurotransmission in the nucleus of solitary tract of the rat by the cannabinoid agonist WIN 55212-2 is not via CB1 or CB2 receptors. Brain Res. 2008;1200:1-9.
    Accorsi-Mendonça, D., Almado, C. E., Dagostin, A. L., Machado, B. H., & Leão, R. M. (2008). Inhibition of spontaneous neurotransmission in the nucleus of solitary tract of the rat by the cannabinoid agonist WIN 55212-2 is not via CB1 or CB2 receptors. Brain Research, 1200, pp. 1-9. doi:10.1016/j.brainres.2008.01.011.
    Accorsi-Mendonça D, et al. Inhibition of Spontaneous Neurotransmission in the Nucleus of Solitary Tract of the Rat By the Cannabinoid Agonist WIN 55212-2 Is Not Via CB1 or CB2 Receptors. Brain Res. 2008 Mar 20;1200:1-9. PubMed PMID: 18308297.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Inhibition of spontaneous neurotransmission in the nucleus of solitary tract of the rat by the cannabinoid agonist WIN 55212-2 is not via CB1 or CB2 receptors. AU - Accorsi-Mendonça,Daniela, AU - Almado,Carlos E L, AU - Dagostin,André L A, AU - Machado,Benedito H, AU - Leão,Ricardo M, Y1 - 2008/01/16/ PY - 2007/08/02/received PY - 2008/01/02/revised PY - 2008/01/04/accepted PY - 2008/3/1/pubmed PY - 2008/7/19/medline PY - 2008/3/1/entrez SP - 1 EP - 9 JF - Brain research JO - Brain Res. VL - 1200 N2 - Cannabinoids have been shown to modulate central autonomic regulation and baroreflex control of blood pressure. Both CB1 and CB2 cannabinoid receptors have been described in the nucleus tractus solitarius (NTS), which receives direct afferent projections of cardiovascular reflexes. In the present study we evaluated the effects of WIN 55212-2 (WIN), a cannabinoid agonist, on fast neurotransmission in the NTS. We recorded spontaneous post-synaptic currents using the whole-cell configuration in NTS cells in brainstem slices from young rats (25-30 days old). Application of 5 microM WIN inhibited the frequency of both glutamatergic and GABAergic sPSCs, without affecting their amplitudes. Effects of WIN were not blocked by application of the CB1 antagonist AM251, the CB2 antagonist AM630 or the vanniloid receptor TRPV1 antagonist AMG9810, suggesting that the effect of WIN is via a non-CB1 non-CB2 receptor. Neither the CB1/CB2 agonist HU210 nor the CB1 agonist ACPA affected the frequency of sPSCs. We conclude WIN inhibits the neurotransmission in the NTS of young rats via a receptor distinct from CB1 or CB2. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/18308297/Inhibition_of_spontaneous_neurotransmission_in_the_nucleus_of_solitary_tract_of_the_rat_by_the_cannabinoid_agonist_WIN_55212_2_is_not_via_CB1_or_CB2_receptors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(08)00042-5 DB - PRIME DP - Unbound Medicine ER -