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Inhibition of spontaneous neurotransmission in the nucleus of solitary tract of the rat by the cannabinoid agonist WIN 55212-2 is not via CB1 or CB2 receptors.
Brain Res 2008; 1200:1-9BR

Abstract

Cannabinoids have been shown to modulate central autonomic regulation and baroreflex control of blood pressure. Both CB1 and CB2 cannabinoid receptors have been described in the nucleus tractus solitarius (NTS), which receives direct afferent projections of cardiovascular reflexes. In the present study we evaluated the effects of WIN 55212-2 (WIN), a cannabinoid agonist, on fast neurotransmission in the NTS. We recorded spontaneous post-synaptic currents using the whole-cell configuration in NTS cells in brainstem slices from young rats (25-30 days old). Application of 5 microM WIN inhibited the frequency of both glutamatergic and GABAergic sPSCs, without affecting their amplitudes. Effects of WIN were not blocked by application of the CB1 antagonist AM251, the CB2 antagonist AM630 or the vanniloid receptor TRPV1 antagonist AMG9810, suggesting that the effect of WIN is via a non-CB1 non-CB2 receptor. Neither the CB1/CB2 agonist HU210 nor the CB1 agonist ACPA affected the frequency of sPSCs. We conclude WIN inhibits the neurotransmission in the NTS of young rats via a receptor distinct from CB1 or CB2.

Authors+Show Affiliations

Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900 14049-900 Ribeirão Preto, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18308297

Citation

Accorsi-Mendonça, Daniela, et al. "Inhibition of Spontaneous Neurotransmission in the Nucleus of Solitary Tract of the Rat By the Cannabinoid Agonist WIN 55212-2 Is Not Via CB1 or CB2 Receptors." Brain Research, vol. 1200, 2008, pp. 1-9.
Accorsi-Mendonça D, Almado CE, Dagostin AL, et al. Inhibition of spontaneous neurotransmission in the nucleus of solitary tract of the rat by the cannabinoid agonist WIN 55212-2 is not via CB1 or CB2 receptors. Brain Res. 2008;1200:1-9.
Accorsi-Mendonça, D., Almado, C. E., Dagostin, A. L., Machado, B. H., & Leão, R. M. (2008). Inhibition of spontaneous neurotransmission in the nucleus of solitary tract of the rat by the cannabinoid agonist WIN 55212-2 is not via CB1 or CB2 receptors. Brain Research, 1200, pp. 1-9. doi:10.1016/j.brainres.2008.01.011.
Accorsi-Mendonça D, et al. Inhibition of Spontaneous Neurotransmission in the Nucleus of Solitary Tract of the Rat By the Cannabinoid Agonist WIN 55212-2 Is Not Via CB1 or CB2 Receptors. Brain Res. 2008 Mar 20;1200:1-9. PubMed PMID: 18308297.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of spontaneous neurotransmission in the nucleus of solitary tract of the rat by the cannabinoid agonist WIN 55212-2 is not via CB1 or CB2 receptors. AU - Accorsi-Mendonça,Daniela, AU - Almado,Carlos E L, AU - Dagostin,André L A, AU - Machado,Benedito H, AU - Leão,Ricardo M, Y1 - 2008/01/16/ PY - 2007/08/02/received PY - 2008/01/02/revised PY - 2008/01/04/accepted PY - 2008/3/1/pubmed PY - 2008/7/19/medline PY - 2008/3/1/entrez SP - 1 EP - 9 JF - Brain research JO - Brain Res. VL - 1200 N2 - Cannabinoids have been shown to modulate central autonomic regulation and baroreflex control of blood pressure. Both CB1 and CB2 cannabinoid receptors have been described in the nucleus tractus solitarius (NTS), which receives direct afferent projections of cardiovascular reflexes. In the present study we evaluated the effects of WIN 55212-2 (WIN), a cannabinoid agonist, on fast neurotransmission in the NTS. We recorded spontaneous post-synaptic currents using the whole-cell configuration in NTS cells in brainstem slices from young rats (25-30 days old). Application of 5 microM WIN inhibited the frequency of both glutamatergic and GABAergic sPSCs, without affecting their amplitudes. Effects of WIN were not blocked by application of the CB1 antagonist AM251, the CB2 antagonist AM630 or the vanniloid receptor TRPV1 antagonist AMG9810, suggesting that the effect of WIN is via a non-CB1 non-CB2 receptor. Neither the CB1/CB2 agonist HU210 nor the CB1 agonist ACPA affected the frequency of sPSCs. We conclude WIN inhibits the neurotransmission in the NTS of young rats via a receptor distinct from CB1 or CB2. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/18308297/Inhibition_of_spontaneous_neurotransmission_in_the_nucleus_of_solitary_tract_of_the_rat_by_the_cannabinoid_agonist_WIN_55212_2_is_not_via_CB1_or_CB2_receptors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(08)00042-5 DB - PRIME DP - Unbound Medicine ER -