Tags

Type your tag names separated by a space and hit enter

A new hydrophilic interaction liquid chromatography tandem mass spectrometry method for the simultaneous determination of seven biogenic amines in cheese.
J Chromatogr A. 2008 Mar 28; 1185(2):296-300.JC

Abstract

An HILIC-PI APCI MS/MS method is developed for the determination of seven biogenic amines (cadaverine, histamine, putrescine, spermidine, spermine, tryptamine and tyramine) in cheese. Their presence and relative amounts give useful information about freshness, level of maturing, quality of storage and cheese typicization. The major drawback in the analysis is represented by the relevant matrix effect and the general unbalanced concentrations of the different amines in cheese. The method proposed represents an improvement with respect to an HPLC-MS/MS method already developed in this laboratory. The new method permits better sensitivities it makes use of a Waters Atlantis HILIC (150.0 mm x 2.1 mm i.d., 3 microm) stationary phase and of a mobile phase of acetonitrile and ammonium formate 50.0 mM in ultrapure water brought to pH 4.00 for formic acid, flowing under gradient conditions. The chromatographic system is interfaced with a 3200QTrap LC-MS/MS system (Applied Biosystem, Foster City, CA, USA) by a Turbo V interface equipped with Heated Nebuliser (APCI) and Turbo Ion Spray (TIS) probes. LOQ values lower than 10 microg L(-1) are obtained. The method is applied in the analysis of Castelmagno cheese.

Authors+Show Affiliations

University of Piemonte Orientale, DISAV Dipartimento di Scienze dell'Ambiente e della Vita, via Bellini 25/G, 15100 Alessandria, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18308327

Citation

Gianotti, V, et al. "A New Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry Method for the Simultaneous Determination of Seven Biogenic Amines in Cheese." Journal of Chromatography. A, vol. 1185, no. 2, 2008, pp. 296-300.
Gianotti V, Chiuminatto U, Mazzucco E, et al. A new hydrophilic interaction liquid chromatography tandem mass spectrometry method for the simultaneous determination of seven biogenic amines in cheese. J Chromatogr A. 2008;1185(2):296-300.
Gianotti, V., Chiuminatto, U., Mazzucco, E., Gosetti, F., Bottaro, M., Frascarolo, P., & Gennaro, M. C. (2008). A new hydrophilic interaction liquid chromatography tandem mass spectrometry method for the simultaneous determination of seven biogenic amines in cheese. Journal of Chromatography. A, 1185(2), 296-300. https://doi.org/10.1016/j.chroma.2008.02.038
Gianotti V, et al. A New Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry Method for the Simultaneous Determination of Seven Biogenic Amines in Cheese. J Chromatogr A. 2008 Mar 28;1185(2):296-300. PubMed PMID: 18308327.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new hydrophilic interaction liquid chromatography tandem mass spectrometry method for the simultaneous determination of seven biogenic amines in cheese. AU - Gianotti,V, AU - Chiuminatto,U, AU - Mazzucco,E, AU - Gosetti,F, AU - Bottaro,M, AU - Frascarolo,P, AU - Gennaro,M C, Y1 - 2008/02/14/ PY - 2007/12/10/received PY - 2008/01/25/revised PY - 2008/02/07/accepted PY - 2008/3/1/pubmed PY - 2008/5/31/medline PY - 2008/3/1/entrez SP - 296 EP - 300 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1185 IS - 2 N2 - An HILIC-PI APCI MS/MS method is developed for the determination of seven biogenic amines (cadaverine, histamine, putrescine, spermidine, spermine, tryptamine and tyramine) in cheese. Their presence and relative amounts give useful information about freshness, level of maturing, quality of storage and cheese typicization. The major drawback in the analysis is represented by the relevant matrix effect and the general unbalanced concentrations of the different amines in cheese. The method proposed represents an improvement with respect to an HPLC-MS/MS method already developed in this laboratory. The new method permits better sensitivities it makes use of a Waters Atlantis HILIC (150.0 mm x 2.1 mm i.d., 3 microm) stationary phase and of a mobile phase of acetonitrile and ammonium formate 50.0 mM in ultrapure water brought to pH 4.00 for formic acid, flowing under gradient conditions. The chromatographic system is interfaced with a 3200QTrap LC-MS/MS system (Applied Biosystem, Foster City, CA, USA) by a Turbo V interface equipped with Heated Nebuliser (APCI) and Turbo Ion Spray (TIS) probes. LOQ values lower than 10 microg L(-1) are obtained. The method is applied in the analysis of Castelmagno cheese. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18308327/A_new_hydrophilic_interaction_liquid_chromatography_tandem_mass_spectrometry_method_for_the_simultaneous_determination_of_seven_biogenic_amines_in_cheese_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)00268-9 DB - PRIME DP - Unbound Medicine ER -