Tags

Type your tag names separated by a space and hit enter

Application of preparative high-speed counter-current chromatography/preparative high-performance liquid chromatography mode in rapid separation of saponins.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Mar 15; 864(1-2):69-77.JC

Abstract

Combined with preparative high-performance liquid chromatography, high-speed counter-current chromatography was employed for isolation and purification of saponins from Gypsophila paniculata L. n-Hexane-n-butanol-methanol-0.02% TFA (1:9:1:9, v/v) was employed as solvent system and 210 nm was chosen as the wavelength of ultraviolet detection for the first time. The research tried to compare HSCCC with prep-HPLC, and further integrated their advantages to improve separation efficiency. Five known triterpene saponins were identified by 13C NMR and ESI-MS and their purities were all above 96%. The results demonstrated that adopted method was a feasible, economical and efficient technique for rapid preparative isolation of saponins.

Authors+Show Affiliations

Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18308647

Citation

Yao, Shun, et al. "Application of Preparative High-speed Counter-current Chromatography/preparative High-performance Liquid Chromatography Mode in Rapid Separation of Saponins." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 864, no. 1-2, 2008, pp. 69-77.
Yao S, Luo J, Huang X, et al. Application of preparative high-speed counter-current chromatography/preparative high-performance liquid chromatography mode in rapid separation of saponins. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;864(1-2):69-77.
Yao, S., Luo, J., Huang, X., & Kong, L. (2008). Application of preparative high-speed counter-current chromatography/preparative high-performance liquid chromatography mode in rapid separation of saponins. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 864(1-2), 69-77. https://doi.org/10.1016/j.jchromb.2008.01.047
Yao S, et al. Application of Preparative High-speed Counter-current Chromatography/preparative High-performance Liquid Chromatography Mode in Rapid Separation of Saponins. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Mar 15;864(1-2):69-77. PubMed PMID: 18308647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of preparative high-speed counter-current chromatography/preparative high-performance liquid chromatography mode in rapid separation of saponins. AU - Yao,Shun, AU - Luo,Jianguang, AU - Huang,Xuefeng, AU - Kong,Lingyi, Y1 - 2008/02/08/ PY - 2007/11/23/received PY - 2008/01/22/revised PY - 2008/01/24/accepted PY - 2008/3/1/pubmed PY - 2008/6/6/medline PY - 2008/3/1/entrez SP - 69 EP - 77 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 864 IS - 1-2 N2 - Combined with preparative high-performance liquid chromatography, high-speed counter-current chromatography was employed for isolation and purification of saponins from Gypsophila paniculata L. n-Hexane-n-butanol-methanol-0.02% TFA (1:9:1:9, v/v) was employed as solvent system and 210 nm was chosen as the wavelength of ultraviolet detection for the first time. The research tried to compare HSCCC with prep-HPLC, and further integrated their advantages to improve separation efficiency. Five known triterpene saponins were identified by 13C NMR and ESI-MS and their purities were all above 96%. The results demonstrated that adopted method was a feasible, economical and efficient technique for rapid preparative isolation of saponins. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18308647/Application_of_preparative_high_speed_counter_current_chromatography/preparative_high_performance_liquid_chromatography_mode_in_rapid_separation_of_saponins_ DB - PRIME DP - Unbound Medicine ER -