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Gpx3-dependent responses against oxidative stress in Saccharomyces cerevisiae.
J Microbiol Biotechnol. 2008 Feb; 18(2):270-82.JM

Abstract

The yeast Saccharomyces cerevisiae has defense mechanisms identical to higher eukaryotes. It offers the potential for genome-wide experimental approaches owing to its smaller genome size and the availability of the complete sequence. It therefore represents an ideal eukaryotic model for studying cellular redox control and oxidative stress responses. S. cerevisiae Yap1 is a well-known transcription factor that is required for H2O2-dependent stress responses. Yap1 is involved in various signaling pathways in an oxidative stress response. The Gpx3 (Orp1/PHGpx3) protein is one of the factors related to these signaling pathways. It plays the role of a transducer that transfers the hydroperoxide signal to Yap1. In this study, using extensive proteomic and bioinformatics analyses, the function of the Gpx3 protein in an adaptive response against oxidative stress was investigated in wild-type, gpx3-deletion mutant, and gpx3-deletion mutant overexpressing Gpx3 protein strains. We identified 30 proteins that are related to the Gpx3- dependent oxidative stress responses and 17 proteins that are changed in a Gpx3-dependent manner regardless of oxidative stress. As expected, H2O2-responsive Gpx3-dependent proteins include a number of antioxidants related with cell rescue and defense. In addition, they contain a variety of proteins related to energy and carbohydrate metabolism, transcription, and protein fate. Based upon the experimental results, it is suggested that Gpx3-dependent stress adaptive response includes the regulation of genes related to the capacity to detoxify oxidants and repair oxidative stress-induced damages affected by Yap1 as well as metabolism and protein fate independent from Yap1.

Authors+Show Affiliations

Translational Research Center, KRIBB, Daejeon 806-333, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18309271

Citation

Kho, Chang Won, et al. "Gpx3-dependent Responses Against Oxidative Stress in Saccharomyces Cerevisiae." Journal of Microbiology and Biotechnology, vol. 18, no. 2, 2008, pp. 270-82.
Kho CW, Lee PY, Bae KH, et al. Gpx3-dependent responses against oxidative stress in Saccharomyces cerevisiae. J Microbiol Biotechnol. 2008;18(2):270-82.
Kho, C. W., Lee, P. Y., Bae, K. H., Kang, S., Cho, S., Lee, D. H., Sun, C. H., Yi, G. S., Park, B. C., & Park, S. G. (2008). Gpx3-dependent responses against oxidative stress in Saccharomyces cerevisiae. Journal of Microbiology and Biotechnology, 18(2), 270-82.
Kho CW, et al. Gpx3-dependent Responses Against Oxidative Stress in Saccharomyces Cerevisiae. J Microbiol Biotechnol. 2008;18(2):270-82. PubMed PMID: 18309271.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gpx3-dependent responses against oxidative stress in Saccharomyces cerevisiae. AU - Kho,Chang Won, AU - Lee,Phil Young, AU - Bae,Kwang-Hee, AU - Kang,Sunghyun, AU - Cho,Sayeon, AU - Lee,Do Hee, AU - Sun,Choong-Hyun, AU - Yi,Gwan-Su, AU - Park,Byoung Chul, AU - Park,Sung Goo, PY - 2008/3/1/pubmed PY - 2008/7/9/medline PY - 2008/3/1/entrez SP - 270 EP - 82 JF - Journal of microbiology and biotechnology JO - J Microbiol Biotechnol VL - 18 IS - 2 N2 - The yeast Saccharomyces cerevisiae has defense mechanisms identical to higher eukaryotes. It offers the potential for genome-wide experimental approaches owing to its smaller genome size and the availability of the complete sequence. It therefore represents an ideal eukaryotic model for studying cellular redox control and oxidative stress responses. S. cerevisiae Yap1 is a well-known transcription factor that is required for H2O2-dependent stress responses. Yap1 is involved in various signaling pathways in an oxidative stress response. The Gpx3 (Orp1/PHGpx3) protein is one of the factors related to these signaling pathways. It plays the role of a transducer that transfers the hydroperoxide signal to Yap1. In this study, using extensive proteomic and bioinformatics analyses, the function of the Gpx3 protein in an adaptive response against oxidative stress was investigated in wild-type, gpx3-deletion mutant, and gpx3-deletion mutant overexpressing Gpx3 protein strains. We identified 30 proteins that are related to the Gpx3- dependent oxidative stress responses and 17 proteins that are changed in a Gpx3-dependent manner regardless of oxidative stress. As expected, H2O2-responsive Gpx3-dependent proteins include a number of antioxidants related with cell rescue and defense. In addition, they contain a variety of proteins related to energy and carbohydrate metabolism, transcription, and protein fate. Based upon the experimental results, it is suggested that Gpx3-dependent stress adaptive response includes the regulation of genes related to the capacity to detoxify oxidants and repair oxidative stress-induced damages affected by Yap1 as well as metabolism and protein fate independent from Yap1. SN - 1017-7825 UR - https://www.unboundmedicine.com/medline/citation/18309271/Gpx3_dependent_responses_against_oxidative_stress_in_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -