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Small outer-membrane lipoprotein, SmpA, is regulated by sigmaE and has a role in cell envelope integrity and virulence of Salmonella enterica serovar Typhimurium.
Microbiology (Reading). 2008 Mar; 154(Pt 3):979-988.M

Abstract

SmpA is a small outer-membrane lipoprotein that is a component of the essential YaeT outer-membrane protein assembly complex. In Salmonella enterica serovar Typhimurium (S. Typhimurium), expression of the smpA gene was shown to be directed by two promoters, smpAp1 and smpAp2. The more distal promoter, smpAp1, is dependent upon the extracytoplasmic stress response sigma factor sigma(E). An smpA null mutant was constructed in S. Typhimurium SL1344 and was shown to be more sensitive than its wild-type parent to growth at high temperature and in the presence of sodium cholate, SDS plus EDTA, and the hydrophobic antibiotic rifampicin. The lack of SmpA in S. Typhimurium elicits a sigma(E)-dependent stress response. These findings are indicative of altered outer-membrane integrity in the smpA mutant, probably due to a defect in outer-membrane protein biogenesis. SmpA was not important for entry or survival within murine macrophages; however, the S. Typhimurium smpA mutant was attenuated in mice by both the oral and parenteral routes of infection, and SmpA appeared to be most important for the growth of S. Typhimurium at systemic sites.

Authors+Show Affiliations

Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK.Institute of Molecular Biology, Slovak Academy of Science, Dubravska cesta 21, 845 51 Bratislava, Slovak Republik.School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK.Institute of Molecular Biology, Slovak Academy of Science, Dubravska cesta 21, 845 51 Bratislava, Slovak Republik.Institute of Molecular Biology, Slovak Academy of Science, Dubravska cesta 21, 845 51 Bratislava, Slovak Republik.Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK.Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK.Institute of Molecular Biology, Slovak Academy of Science, Dubravska cesta 21, 845 51 Bratislava, Slovak Republik.Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18310044

Citation

Lewis, Claire, et al. "Small Outer-membrane Lipoprotein, SmpA, Is Regulated By sigmaE and Has a Role in Cell Envelope Integrity and Virulence of Salmonella Enterica Serovar Typhimurium." Microbiology (Reading, England), vol. 154, no. Pt 3, 2008, pp. 979-988.
Lewis C, Skovierova H, Rowley G, et al. Small outer-membrane lipoprotein, SmpA, is regulated by sigmaE and has a role in cell envelope integrity and virulence of Salmonella enterica serovar Typhimurium. Microbiology (Reading). 2008;154(Pt 3):979-988.
Lewis, C., Skovierova, H., Rowley, G., Rezuchova, B., Homerova, D., Stevenson, A., Sherry, A., Kormanec, J., & Roberts, M. (2008). Small outer-membrane lipoprotein, SmpA, is regulated by sigmaE and has a role in cell envelope integrity and virulence of Salmonella enterica serovar Typhimurium. Microbiology (Reading, England), 154(Pt 3), 979-988. https://doi.org/10.1099/mic.0.2007/011999-0
Lewis C, et al. Small Outer-membrane Lipoprotein, SmpA, Is Regulated By sigmaE and Has a Role in Cell Envelope Integrity and Virulence of Salmonella Enterica Serovar Typhimurium. Microbiology (Reading). 2008;154(Pt 3):979-988. PubMed PMID: 18310044.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Small outer-membrane lipoprotein, SmpA, is regulated by sigmaE and has a role in cell envelope integrity and virulence of Salmonella enterica serovar Typhimurium. AU - Lewis,Claire, AU - Skovierova,Henrieta, AU - Rowley,Gary, AU - Rezuchova,Bronislava, AU - Homerova,Dagmar, AU - Stevenson,Andrew, AU - Sherry,Aileen, AU - Kormanec,Jan, AU - Roberts,Mark, PY - 2008/3/4/pubmed PY - 2008/5/2/medline PY - 2008/3/4/entrez SP - 979 EP - 988 JF - Microbiology (Reading, England) JO - Microbiology (Reading) VL - 154 IS - Pt 3 N2 - SmpA is a small outer-membrane lipoprotein that is a component of the essential YaeT outer-membrane protein assembly complex. In Salmonella enterica serovar Typhimurium (S. Typhimurium), expression of the smpA gene was shown to be directed by two promoters, smpAp1 and smpAp2. The more distal promoter, smpAp1, is dependent upon the extracytoplasmic stress response sigma factor sigma(E). An smpA null mutant was constructed in S. Typhimurium SL1344 and was shown to be more sensitive than its wild-type parent to growth at high temperature and in the presence of sodium cholate, SDS plus EDTA, and the hydrophobic antibiotic rifampicin. The lack of SmpA in S. Typhimurium elicits a sigma(E)-dependent stress response. These findings are indicative of altered outer-membrane integrity in the smpA mutant, probably due to a defect in outer-membrane protein biogenesis. SmpA was not important for entry or survival within murine macrophages; however, the S. Typhimurium smpA mutant was attenuated in mice by both the oral and parenteral routes of infection, and SmpA appeared to be most important for the growth of S. Typhimurium at systemic sites. SN - 1350-0872 UR - https://www.unboundmedicine.com/medline/citation/18310044/Small_outer_membrane_lipoprotein_SmpA_is_regulated_by_sigmaE_and_has_a_role_in_cell_envelope_integrity_and_virulence_of_Salmonella_enterica_serovar_Typhimurium_ L2 - http://mic.microbiologyresearch.org/pubmed/content/journal/micro/10.1099/mic.0.2007/011999-0 DB - PRIME DP - Unbound Medicine ER -