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A sensitive determination method for carbendazim and thiabendazole in apples by solid-phase microextraction-high performance liquid chromatography with fluorescence detection.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008 Mar; 25(3):314-9.FA

Abstract

A new analytical method for the determination of carbendazim (MBC) and thiabendazole (TBZ) in apples is reported, based on solid-phase microextraction (SPME) coupling HPLC with fluorescence detection. The main SPME and HPLC experimental conditions were optimized. The apples were first blended and centrifuged. Then, an aliquot of the resulting solution was subjected to SPME on a 60 microm polydimethylsiloxane/divinylbenzene (PDMS/DVB) fibre for 35 min at room temperature with the solution being stirred at 1100 rev min(-1). The extracted pesticides on the SPME fibre were desorbed in the mobile phase into the SPME/HPLC interface for HPLC analysis. The method was linear over the range 0.01-1 mg kg(-1) in apples for both MBC and TBZ, with detection limits of 0.005 and 0.003 mg kg(-1) and correlation coefficients of 0.9995 and 0.9998, respectively. The average recoveries for MBC and TBZ were 91.5 and 92.3% with the relative standard deviations (RSD) of 4.7 and 4.1% at the 0.1 mg kg(-1) level, and 94.6 and 96.1% with RSD of 3.3 and 3.8% at the 0.5 mg kg(-1) level, respectively. The method is simple, sensitive, organic solvent-free and is suitable for the determination of MBC and TBZ in apples.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Hu Y
Key Laboratory of Bioinorganic Chemistry, College of Science, Agricultural University of Hebei, Baoding 071001, China.
Yang X
No affiliation info available
Wang C
No affiliation info available
Zhao J
No affiliation info available
Li W
No affiliation info available
Wang Z
No affiliation info available

MeSH

BenzimidazolesCarbamatesChromatography, High Pressure LiquidFood ContaminationFungicides, IndustrialMalusPesticide ResiduesSolid Phase MicroextractionThiabendazole

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18311622

Citation

Hu, Yanxue, et al. "A Sensitive Determination Method for Carbendazim and Thiabendazole in Apples By Solid-phase Microextraction-high Performance Liquid Chromatography With Fluorescence Detection." Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, vol. 25, no. 3, 2008, pp. 314-9.
Hu Y, Yang X, Wang C, et al. A sensitive determination method for carbendazim and thiabendazole in apples by solid-phase microextraction-high performance liquid chromatography with fluorescence detection. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008;25(3):314-9.
Hu, Y., Yang, X., Wang, C., Zhao, J., Li, W., & Wang, Z. (2008). A sensitive determination method for carbendazim and thiabendazole in apples by solid-phase microextraction-high performance liquid chromatography with fluorescence detection. Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 25(3), 314-9. https://doi.org/10.1080/02652030701581864
Hu Y, et al. A Sensitive Determination Method for Carbendazim and Thiabendazole in Apples By Solid-phase Microextraction-high Performance Liquid Chromatography With Fluorescence Detection. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008;25(3):314-9. PubMed PMID: 18311622.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A sensitive determination method for carbendazim and thiabendazole in apples by solid-phase microextraction-high performance liquid chromatography with fluorescence detection. AU - Hu,Yanxue, AU - Yang,Xiumin, AU - Wang,Chun, AU - Zhao,Jin, AU - Li,Weining, AU - Wang,Zhi, PY - 2008/3/4/pubmed PY - 2008/8/22/medline PY - 2008/3/4/entrez SP - 314 EP - 9 JF - Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment JO - Food Addit Contam Part A Chem Anal Control Expo Risk Assess VL - 25 IS - 3 N2 - A new analytical method for the determination of carbendazim (MBC) and thiabendazole (TBZ) in apples is reported, based on solid-phase microextraction (SPME) coupling HPLC with fluorescence detection. The main SPME and HPLC experimental conditions were optimized. The apples were first blended and centrifuged. Then, an aliquot of the resulting solution was subjected to SPME on a 60 microm polydimethylsiloxane/divinylbenzene (PDMS/DVB) fibre for 35 min at room temperature with the solution being stirred at 1100 rev min(-1). The extracted pesticides on the SPME fibre were desorbed in the mobile phase into the SPME/HPLC interface for HPLC analysis. The method was linear over the range 0.01-1 mg kg(-1) in apples for both MBC and TBZ, with detection limits of 0.005 and 0.003 mg kg(-1) and correlation coefficients of 0.9995 and 0.9998, respectively. The average recoveries for MBC and TBZ were 91.5 and 92.3% with the relative standard deviations (RSD) of 4.7 and 4.1% at the 0.1 mg kg(-1) level, and 94.6 and 96.1% with RSD of 3.3 and 3.8% at the 0.5 mg kg(-1) level, respectively. The method is simple, sensitive, organic solvent-free and is suitable for the determination of MBC and TBZ in apples. SN - 1944-0057 UR - https://www.unboundmedicine.com/medline/citation/18311622/A_sensitive_determination_method_for_carbendazim_and_thiabendazole_in_apples_by_solid_phase_microextraction_high_performance_liquid_chromatography_with_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -
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