Tags

Type your tag names separated by a space and hit enter

Characterization of myenteric neuropathy in the jejunum of spontaneously diabetic BB-rats.
Neurogastroenterol Motil 2008; 20(7):818-28NM

Abstract

Decreased gastric expression and function of neuronal nitric oxide synthase (nNOS) has been proposed as a potential mechanism underlying diabetic gastroparesis. As gastric nNOS expression is vagally controlled, these changes might occur secondarily to vagal neuropathy. In addition, it is unclear whether other inhibitory neurotransmitters are also involved. We used the type 1 diabetic BioBreeding (BB)-rat model to study jejunal motor control and nNOS expression, which is independent of the vagus. Jejunal segments were used for in vitro contractility studies, and measurement of nNOS expression after 8 or 16 weeks of diabetes compared with age- and sex-matched controls. Unlike electrical field stimulation and acetylcholine (ACh)-induced contractions, non-adrenergic non-cholinergic (NANC) relaxations were significantly reduced in diabetic rats. In contrast to control rats, NANC relaxations in diabetic rats were N(omega)-nitro-L-arginine methyl ester (L-NAME) insensitive. Jejunal nNOS expression was significantly decreased in diabetic rats. Both in diabetic and in control animals, L-NAME resistant relaxations were sensitive to P(2)-receptor antagonists. In the jejunum of spontaneously diabetic rats, decreased nitric oxide responsiveness and decreased nNOS protein expression occur while purinergic transmission is unaffected. These findings indicate that nitrergic enteric neuropathy may be a primary dysfunction in diabetes, independent from vagal dysfunction.

Authors+Show Affiliations

Center for Gastroenterological Research, Catholic University Leuven, Leuven, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18312542

Citation

Zandecki, M, et al. "Characterization of Myenteric Neuropathy in the Jejunum of Spontaneously Diabetic BB-rats." Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society, vol. 20, no. 7, 2008, pp. 818-28.
Zandecki M, Vanden Berghe P, Depoortere I, et al. Characterization of myenteric neuropathy in the jejunum of spontaneously diabetic BB-rats. Neurogastroenterol Motil. 2008;20(7):818-28.
Zandecki, M., Vanden Berghe, P., Depoortere, I., Geboes, K., Peeters, T., Janssens, J., & Tack, J. (2008). Characterization of myenteric neuropathy in the jejunum of spontaneously diabetic BB-rats. Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society, 20(7), pp. 818-28. doi:10.1111/j.1365-2982.2008.01091.x.
Zandecki M, et al. Characterization of Myenteric Neuropathy in the Jejunum of Spontaneously Diabetic BB-rats. Neurogastroenterol Motil. 2008;20(7):818-28. PubMed PMID: 18312542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of myenteric neuropathy in the jejunum of spontaneously diabetic BB-rats. AU - Zandecki,M, AU - Vanden Berghe,P, AU - Depoortere,I, AU - Geboes,K, AU - Peeters,T, AU - Janssens,J, AU - Tack,J, Y1 - 2008/02/25/ PY - 2008/3/4/pubmed PY - 2008/8/13/medline PY - 2008/3/4/entrez SP - 818 EP - 28 JF - Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society JO - Neurogastroenterol. Motil. VL - 20 IS - 7 N2 - Decreased gastric expression and function of neuronal nitric oxide synthase (nNOS) has been proposed as a potential mechanism underlying diabetic gastroparesis. As gastric nNOS expression is vagally controlled, these changes might occur secondarily to vagal neuropathy. In addition, it is unclear whether other inhibitory neurotransmitters are also involved. We used the type 1 diabetic BioBreeding (BB)-rat model to study jejunal motor control and nNOS expression, which is independent of the vagus. Jejunal segments were used for in vitro contractility studies, and measurement of nNOS expression after 8 or 16 weeks of diabetes compared with age- and sex-matched controls. Unlike electrical field stimulation and acetylcholine (ACh)-induced contractions, non-adrenergic non-cholinergic (NANC) relaxations were significantly reduced in diabetic rats. In contrast to control rats, NANC relaxations in diabetic rats were N(omega)-nitro-L-arginine methyl ester (L-NAME) insensitive. Jejunal nNOS expression was significantly decreased in diabetic rats. Both in diabetic and in control animals, L-NAME resistant relaxations were sensitive to P(2)-receptor antagonists. In the jejunum of spontaneously diabetic rats, decreased nitric oxide responsiveness and decreased nNOS protein expression occur while purinergic transmission is unaffected. These findings indicate that nitrergic enteric neuropathy may be a primary dysfunction in diabetes, independent from vagal dysfunction. SN - 1365-2982 UR - https://www.unboundmedicine.com/medline/citation/18312542/Characterization_of_myenteric_neuropathy_in_the_jejunum_of_spontaneously_diabetic_BB_rats_ L2 - https://doi.org/10.1111/j.1365-2982.2008.01091.x DB - PRIME DP - Unbound Medicine ER -