TY - JOUR T1 - Genetic typing of the 56-kDa type-specific antigen gene of contemporary Orientia tsutsugamushi isolates causing human scrub typhus at two sites in north-eastern and western Thailand. AU - Blacksell,Stuart D, AU - Luksameetanasan,Rungnapa, AU - Kalambaheti,Thareerat, AU - Aukkanit,Nuntipa, AU - Paris,Daniel H, AU - McGready,Rose, AU - Nosten,François, AU - Peacock,Sharon J, AU - Day,Nicholas P J, Y1 - 2008/02/27/ PY - 2008/3/4/pubmed PY - 2008/8/14/medline PY - 2008/3/4/entrez SP - 335 EP - 42 JF - FEMS immunology and medical microbiology JO - FEMS Immunol Med Microbiol VL - 52 IS - 3 N2 - Orientia tsutsugamushi is the causative agent of scrub typhus, a major cause of febrile illness in the rural areas of Southeast Asia. Twenty-three strains of O. tsutsugamushi were isolated from patients with scrub typhus in north-east (Udorn Thani province) and western Thailand (Tak province) between 2003 and 2005. The isolates were characterized by sequencing the entire ORF of the 56-kDa-type-specific antigen gene, followed by phylogenetic analysis. The majority (15/23) of isolates clustered with the Karp-type strain, six with a Gilliam-type strain and one each with the TA716- and TA763-type strains. Overall, there was considerable diversity in sequence, comparable to that seen in strains from across the rest of the scrub typhus-endemic world. There was no significant difference in the distributions of strains between the two provinces (P=0.08, Fisher's exact) nor a temporal change in distribution with year of isolation (P=0.80, Fisher's exact). Within this diversity there were also examples of isolates with identical 56-kDa genotypes that were cultured from patients from the same geographical areas. SN - 0928-8244 UR - https://www.unboundmedicine.com/medline/citation/18312580/Genetic_typing_of_the_56_kDa_type_specific_antigen_gene_of_contemporary_Orientia_tsutsugamushi_isolates_causing_human_scrub_typhus_at_two_sites_in_north_eastern_and_western_Thailand_ DB - PRIME DP - Unbound Medicine ER -