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Genetic typing of the 56-kDa type-specific antigen gene of contemporary Orientia tsutsugamushi isolates causing human scrub typhus at two sites in north-eastern and western Thailand.
FEMS Immunol Med Microbiol. 2008 Apr; 52(3):335-42.FI

Abstract

Orientia tsutsugamushi is the causative agent of scrub typhus, a major cause of febrile illness in the rural areas of Southeast Asia. Twenty-three strains of O. tsutsugamushi were isolated from patients with scrub typhus in north-east (Udorn Thani province) and western Thailand (Tak province) between 2003 and 2005. The isolates were characterized by sequencing the entire ORF of the 56-kDa-type-specific antigen gene, followed by phylogenetic analysis. The majority (15/23) of isolates clustered with the Karp-type strain, six with a Gilliam-type strain and one each with the TA716- and TA763-type strains. Overall, there was considerable diversity in sequence, comparable to that seen in strains from across the rest of the scrub typhus-endemic world. There was no significant difference in the distributions of strains between the two provinces (P=0.08, Fisher's exact) nor a temporal change in distribution with year of isolation (P=0.80, Fisher's exact). Within this diversity there were also examples of isolates with identical 56-kDa genotypes that were cultured from patients from the same geographical areas.

Authors+Show Affiliations

Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand. stuart@tropmedres.acNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18312580

Citation

Blacksell, Stuart D., et al. "Genetic Typing of the 56-kDa Type-specific Antigen Gene of Contemporary Orientia Tsutsugamushi Isolates Causing Human Scrub Typhus at Two Sites in North-eastern and Western Thailand." FEMS Immunology and Medical Microbiology, vol. 52, no. 3, 2008, pp. 335-42.
Blacksell SD, Luksameetanasan R, Kalambaheti T, et al. Genetic typing of the 56-kDa type-specific antigen gene of contemporary Orientia tsutsugamushi isolates causing human scrub typhus at two sites in north-eastern and western Thailand. FEMS Immunol Med Microbiol. 2008;52(3):335-42.
Blacksell, S. D., Luksameetanasan, R., Kalambaheti, T., Aukkanit, N., Paris, D. H., McGready, R., Nosten, F., Peacock, S. J., & Day, N. P. (2008). Genetic typing of the 56-kDa type-specific antigen gene of contemporary Orientia tsutsugamushi isolates causing human scrub typhus at two sites in north-eastern and western Thailand. FEMS Immunology and Medical Microbiology, 52(3), 335-42. https://doi.org/10.1111/j.1574-695X.2007.00375.x
Blacksell SD, et al. Genetic Typing of the 56-kDa Type-specific Antigen Gene of Contemporary Orientia Tsutsugamushi Isolates Causing Human Scrub Typhus at Two Sites in North-eastern and Western Thailand. FEMS Immunol Med Microbiol. 2008;52(3):335-42. PubMed PMID: 18312580.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic typing of the 56-kDa type-specific antigen gene of contemporary Orientia tsutsugamushi isolates causing human scrub typhus at two sites in north-eastern and western Thailand. AU - Blacksell,Stuart D, AU - Luksameetanasan,Rungnapa, AU - Kalambaheti,Thareerat, AU - Aukkanit,Nuntipa, AU - Paris,Daniel H, AU - McGready,Rose, AU - Nosten,François, AU - Peacock,Sharon J, AU - Day,Nicholas P J, Y1 - 2008/02/27/ PY - 2008/3/4/pubmed PY - 2008/8/14/medline PY - 2008/3/4/entrez SP - 335 EP - 42 JF - FEMS immunology and medical microbiology JO - FEMS Immunol Med Microbiol VL - 52 IS - 3 N2 - Orientia tsutsugamushi is the causative agent of scrub typhus, a major cause of febrile illness in the rural areas of Southeast Asia. Twenty-three strains of O. tsutsugamushi were isolated from patients with scrub typhus in north-east (Udorn Thani province) and western Thailand (Tak province) between 2003 and 2005. The isolates were characterized by sequencing the entire ORF of the 56-kDa-type-specific antigen gene, followed by phylogenetic analysis. The majority (15/23) of isolates clustered with the Karp-type strain, six with a Gilliam-type strain and one each with the TA716- and TA763-type strains. Overall, there was considerable diversity in sequence, comparable to that seen in strains from across the rest of the scrub typhus-endemic world. There was no significant difference in the distributions of strains between the two provinces (P=0.08, Fisher's exact) nor a temporal change in distribution with year of isolation (P=0.80, Fisher's exact). Within this diversity there were also examples of isolates with identical 56-kDa genotypes that were cultured from patients from the same geographical areas. SN - 0928-8244 UR - https://www.unboundmedicine.com/medline/citation/18312580/Genetic_typing_of_the_56_kDa_type_specific_antigen_gene_of_contemporary_Orientia_tsutsugamushi_isolates_causing_human_scrub_typhus_at_two_sites_in_north_eastern_and_western_Thailand_ DB - PRIME DP - Unbound Medicine ER -