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DNA repair synthesis facilitates RAD52-mediated second-end capture during DSB repair.
Mol Cell. 2008 Feb 29; 29(4):510-6.MC

Abstract

Homologous recombination (HR) is essential for the repair of DNA double-strand breaks (DSBs) in mitotic and meiotic cells. HR occurs through a series of steps involving DSB resection, invasion of single-stranded DNA into homologous duplex DNA to form a D loop, repair synthesis, and second-end capture. We show that DNA repair synthesis, catalyzed by human DNA polymerase eta (poleta) acting upon the priming strand of a D loop, leads to capture and annealing of the second end of a resected DSB in reactions mediated by RAD52 protein. Second-end capture products were not detected when poleta was replaced by other polymerases such as poldelta or poliota. RAD52 could not be replaced by RAD51. We also found that the RAD52-dependent reaction was stimulated by the single-strand binding protein RPA, but not by E. coli SSB. Following repair synthesis and second-end capture, de novo DNA synthesis was observed from the captured second DNA end.

Authors+Show Affiliations

Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18313388

Citation

McIlwraith, Michael J., and Stephen C. West. "DNA Repair Synthesis Facilitates RAD52-mediated Second-end Capture During DSB Repair." Molecular Cell, vol. 29, no. 4, 2008, pp. 510-6.
McIlwraith MJ, West SC. DNA repair synthesis facilitates RAD52-mediated second-end capture during DSB repair. Mol Cell. 2008;29(4):510-6.
McIlwraith, M. J., & West, S. C. (2008). DNA repair synthesis facilitates RAD52-mediated second-end capture during DSB repair. Molecular Cell, 29(4), 510-6. https://doi.org/10.1016/j.molcel.2007.11.037
McIlwraith MJ, West SC. DNA Repair Synthesis Facilitates RAD52-mediated Second-end Capture During DSB Repair. Mol Cell. 2008 Feb 29;29(4):510-6. PubMed PMID: 18313388.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA repair synthesis facilitates RAD52-mediated second-end capture during DSB repair. AU - McIlwraith,Michael J, AU - West,Stephen C, PY - 2007/06/22/received PY - 2007/09/27/revised PY - 2007/11/05/accepted PY - 2008/3/4/pubmed PY - 2008/4/30/medline PY - 2008/3/4/entrez SP - 510 EP - 6 JF - Molecular cell JO - Mol Cell VL - 29 IS - 4 N2 - Homologous recombination (HR) is essential for the repair of DNA double-strand breaks (DSBs) in mitotic and meiotic cells. HR occurs through a series of steps involving DSB resection, invasion of single-stranded DNA into homologous duplex DNA to form a D loop, repair synthesis, and second-end capture. We show that DNA repair synthesis, catalyzed by human DNA polymerase eta (poleta) acting upon the priming strand of a D loop, leads to capture and annealing of the second end of a resected DSB in reactions mediated by RAD52 protein. Second-end capture products were not detected when poleta was replaced by other polymerases such as poldelta or poliota. RAD52 could not be replaced by RAD51. We also found that the RAD52-dependent reaction was stimulated by the single-strand binding protein RPA, but not by E. coli SSB. Following repair synthesis and second-end capture, de novo DNA synthesis was observed from the captured second DNA end. SN - 1097-4164 UR - https://www.unboundmedicine.com/medline/citation/18313388/DNA_repair_synthesis_facilitates_RAD52_mediated_second_end_capture_during_DSB_repair_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1097-2765(08)00013-0 DB - PRIME DP - Unbound Medicine ER -