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[Optimization of the gene composition of influenza H5 virus hemagglutinin-containing reassortants and their efficacy in immune cross-protection experiments].
Vopr Virusol. 2008 Jan-Feb; 53(1):24-7.VV

Abstract

The reassortant described in the authors' previous paper contained 6 genes originating from the high-yield virus A/Puerto Rico/8/34 (H1N1) and the genes of hemagglutinin (HA) and neuraminidase (NA) of the low-pathogenic avian influenza A/Duck/Primorie/2621/2001 (H5N2) (6:2 reassortant). The reassortant was used for the backcrossing with the parent avian virus in order to optimize the gene composition. Genotyping of the highest-yield second-generation reassortment indicated that it had obtained the PB1, HA, and NA genes from the virus A/Duck/Primorie/ 2621/2001 and the other genes received the genes from the virus A/Puerto Rico/8/34 (5:3 reassortant). The yield produced in the embryonated chicken eggs by the 5:3 reassortant was higher than that produced by the 6:2 reassortant although it did not achieve the reproduction of the parent virus A/Puerto Rico/8/34. Murine immunization with the inactivated reassortant containing the HA and NA genes of the virus A/Duck/Primorie/2621/2001 (H5N2) provided an efficient protection against the virus containing HA and NA of a recent H5N1 strain.

Authors

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Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

rus

PubMed ID

18318131

Citation

Rudneva, I A., et al. "[Optimization of the Gene Composition of Influenza H5 Virus Hemagglutinin-containing Reassortants and Their Efficacy in Immune Cross-protection Experiments]." Voprosy Virusologii, vol. 53, no. 1, 2008, pp. 24-7.
Rudneva IA, Kaverin NV, Timofeeva TA, et al. [Optimization of the gene composition of influenza H5 virus hemagglutinin-containing reassortants and their efficacy in immune cross-protection experiments]. Vopr Virusol. 2008;53(1):24-7.
Rudneva, I. A., Kaverin, N. V., Timofeeva, T. A., Shilov, A. A., Varich, N. L., Kochergin-Nikitskiĭ, K. S., Krylov, P. S., & L'vov, D. K. (2008). [Optimization of the gene composition of influenza H5 virus hemagglutinin-containing reassortants and their efficacy in immune cross-protection experiments]. Voprosy Virusologii, 53(1), 24-7.
Rudneva IA, et al. [Optimization of the Gene Composition of Influenza H5 Virus Hemagglutinin-containing Reassortants and Their Efficacy in Immune Cross-protection Experiments]. Vopr Virusol. 2008 Jan-Feb;53(1):24-7. PubMed PMID: 18318131.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Optimization of the gene composition of influenza H5 virus hemagglutinin-containing reassortants and their efficacy in immune cross-protection experiments]. AU - Rudneva,I A, AU - Kaverin,N V, AU - Timofeeva,T A, AU - Shilov,A A, AU - Varich,N L, AU - Kochergin-Nikitskiĭ,K S, AU - Krylov,P S, AU - L'vov,D K, PY - 2008/3/6/pubmed PY - 2008/6/24/medline PY - 2008/3/6/entrez SP - 24 EP - 7 JF - Voprosy virusologii JO - Vopr Virusol VL - 53 IS - 1 N2 - The reassortant described in the authors' previous paper contained 6 genes originating from the high-yield virus A/Puerto Rico/8/34 (H1N1) and the genes of hemagglutinin (HA) and neuraminidase (NA) of the low-pathogenic avian influenza A/Duck/Primorie/2621/2001 (H5N2) (6:2 reassortant). The reassortant was used for the backcrossing with the parent avian virus in order to optimize the gene composition. Genotyping of the highest-yield second-generation reassortment indicated that it had obtained the PB1, HA, and NA genes from the virus A/Duck/Primorie/ 2621/2001 and the other genes received the genes from the virus A/Puerto Rico/8/34 (5:3 reassortant). The yield produced in the embryonated chicken eggs by the 5:3 reassortant was higher than that produced by the 6:2 reassortant although it did not achieve the reproduction of the parent virus A/Puerto Rico/8/34. Murine immunization with the inactivated reassortant containing the HA and NA genes of the virus A/Duck/Primorie/2621/2001 (H5N2) provided an efficient protection against the virus containing HA and NA of a recent H5N1 strain. SN - 0507-4088 UR - https://www.unboundmedicine.com/medline/citation/18318131/[Optimization_of_the_gene_composition_of_influenza_H5_virus_hemagglutinin_containing_reassortants_and_their_efficacy_in_immune_cross_protection_experiments]_ L2 - https://medlineplus.gov/vaccines.html DB - PRIME DP - Unbound Medicine ER -