TY - JOUR T1 - [Optimization of the gene composition of influenza H5 virus hemagglutinin-containing reassortants and their efficacy in immune cross-protection experiments]. AU - Rudneva,I A, AU - Kaverin,N V, AU - Timofeeva,T A, AU - Shilov,A A, AU - Varich,N L, AU - Kochergin-Nikitskiĭ,K S, AU - Krylov,P S, AU - L'vov,D K, PY - 2008/3/6/pubmed PY - 2008/6/24/medline PY - 2008/3/6/entrez SP - 24 EP - 7 JF - Voprosy virusologii JO - Vopr Virusol VL - 53 IS - 1 N2 - The reassortant described in the authors' previous paper contained 6 genes originating from the high-yield virus A/Puerto Rico/8/34 (H1N1) and the genes of hemagglutinin (HA) and neuraminidase (NA) of the low-pathogenic avian influenza A/Duck/Primorie/2621/2001 (H5N2) (6:2 reassortant). The reassortant was used for the backcrossing with the parent avian virus in order to optimize the gene composition. Genotyping of the highest-yield second-generation reassortment indicated that it had obtained the PB1, HA, and NA genes from the virus A/Duck/Primorie/ 2621/2001 and the other genes received the genes from the virus A/Puerto Rico/8/34 (5:3 reassortant). The yield produced in the embryonated chicken eggs by the 5:3 reassortant was higher than that produced by the 6:2 reassortant although it did not achieve the reproduction of the parent virus A/Puerto Rico/8/34. Murine immunization with the inactivated reassortant containing the HA and NA genes of the virus A/Duck/Primorie/2621/2001 (H5N2) provided an efficient protection against the virus containing HA and NA of a recent H5N1 strain. SN - 0507-4088 UR - https://www.unboundmedicine.com/medline/citation/18318131/[Optimization_of_the_gene_composition_of_influenza_H5_virus_hemagglutinin_containing_reassortants_and_their_efficacy_in_immune_cross_protection_experiments]_ L2 - https://medlineplus.gov/immunization.html DB - PRIME DP - Unbound Medicine ER -