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A recombinant form of chagasin from Trypanosoma cruzi: inhibitory activity on insect cysteine proteinases.
Pest Manag Sci. 2008 Jul; 64(7):755-60.PM

Abstract

BACKGROUND

The activity of the major digestive cysteine proteinase detected in the intestinal tract of larvae of the bean weevil, Acanthoscelides obtectus (Say), was efficiently inhibited by the well-characterized cysteine proteinase synthetic inhibitor E-64 and also by a recombinant form of chagasin (r-chagasin), a tight-binding cysteine proteinase inhibitor protein from Trypanosoma cruzi.

RESULTS

Incorporation of r-chagasin into an artificial diet system at 0.1 g kg(-1) retarded growth rate, decreased larval survival and led to complete mortality of A. obtectus at the end of the trial. The observed differences in growth rates occurred particularly in the first and second development stages. Artificial seeds containing high levels of r-chagasin (0.5-30 g kg(-1)) completely inhibited larval penetration.

CONCLUSION

Together, the results reported in this paper support the hypothesis that the inhibitory activity of r-chagasin towards the major insect gut cysteine proteinase in vitro and in vivo is an accurate prediction of its insecticidal effects. The selectivity of this inhibitor against insect digestive proteinases supports the key role in parasite virulence by affecting the endogenous proteinase activity in its natural host.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Dos Santos Monteiro AC
Embrapa Recursos Genéticos e Biotecnologia, PqEB, Final W5 Norte, 70770-900, Brasília, DF, Brazil.
de Oliveira Neto OB
No affiliation info available
Del Sarto RP
No affiliation info available
de Magalhães MT
No affiliation info available
Lima JN
No affiliation info available
Lacerda AF
No affiliation info available
Oliveira RS
No affiliation info available
Scharfstein J
No affiliation info available
da Silva MC
No affiliation info available
Valencia JW
No affiliation info available
Jiménez AV
No affiliation info available
Grossi-de-Sa MF
No affiliation info available

MeSH

AnimalsColeopteraCysteine EndopeptidasesCysteine Proteinase InhibitorsGastrointestinal TractInsect ProteinsLarvaPest Control, BiologicalProtozoan ProteinsRecombinant Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18318460

Citation

Dos Santos Monteiro, Ana Carolina, et al. "A Recombinant Form of Chagasin From Trypanosoma Cruzi: Inhibitory Activity On Insect Cysteine Proteinases." Pest Management Science, vol. 64, no. 7, 2008, pp. 755-60.
Dos Santos Monteiro AC, de Oliveira Neto OB, Del Sarto RP, et al. A recombinant form of chagasin from Trypanosoma cruzi: inhibitory activity on insect cysteine proteinases. Pest Manag Sci. 2008;64(7):755-60.
Dos Santos Monteiro, A. C., de Oliveira Neto, O. B., Del Sarto, R. P., de Magalhães, M. T., Lima, J. N., Lacerda, A. F., Oliveira, R. S., Scharfstein, J., da Silva, M. C., Valencia, J. W., Jiménez, A. V., & Grossi-de-Sa, M. F. (2008). A recombinant form of chagasin from Trypanosoma cruzi: inhibitory activity on insect cysteine proteinases. Pest Management Science, 64(7), 755-60. https://doi.org/10.1002/ps.1553
Dos Santos Monteiro AC, et al. A Recombinant Form of Chagasin From Trypanosoma Cruzi: Inhibitory Activity On Insect Cysteine Proteinases. Pest Manag Sci. 2008;64(7):755-60. PubMed PMID: 18318460.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A recombinant form of chagasin from Trypanosoma cruzi: inhibitory activity on insect cysteine proteinases. AU - Dos Santos Monteiro,Ana Carolina, AU - de Oliveira Neto,Osmundo Brilhante, AU - Del Sarto,Rafael Perseghini, AU - de Magalhães,Mariana Torquato Q, AU - Lima,Janaina Nascimento, AU - Lacerda,Ariane Ferreira, AU - Oliveira,Raquel Sampaio, AU - Scharfstein,Julio, AU - da Silva,Maria Cristina Mattar, AU - Valencia,Jorge W Arboleda, AU - Jiménez,Arnubio Valencia, AU - Grossi-de-Sa,Maria Fatima, PY - 2008/3/6/pubmed PY - 2008/9/6/medline PY - 2008/3/6/entrez SP - 755 EP - 60 JF - Pest management science JO - Pest Manag Sci VL - 64 IS - 7 N2 - BACKGROUND: The activity of the major digestive cysteine proteinase detected in the intestinal tract of larvae of the bean weevil, Acanthoscelides obtectus (Say), was efficiently inhibited by the well-characterized cysteine proteinase synthetic inhibitor E-64 and also by a recombinant form of chagasin (r-chagasin), a tight-binding cysteine proteinase inhibitor protein from Trypanosoma cruzi. RESULTS: Incorporation of r-chagasin into an artificial diet system at 0.1 g kg(-1) retarded growth rate, decreased larval survival and led to complete mortality of A. obtectus at the end of the trial. The observed differences in growth rates occurred particularly in the first and second development stages. Artificial seeds containing high levels of r-chagasin (0.5-30 g kg(-1)) completely inhibited larval penetration. CONCLUSION: Together, the results reported in this paper support the hypothesis that the inhibitory activity of r-chagasin towards the major insect gut cysteine proteinase in vitro and in vivo is an accurate prediction of its insecticidal effects. The selectivity of this inhibitor against insect digestive proteinases supports the key role in parasite virulence by affecting the endogenous proteinase activity in its natural host. SN - 1526-498X UR - https://www.unboundmedicine.com/medline/citation/18318460/A_recombinant_form_of_chagasin_from_Trypanosoma_cruzi:_inhibitory_activity_on_insect_cysteine_proteinases_ DB - PRIME DP - Unbound Medicine ER -