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The inheritance of mtDNA in lager brewing strains.
FEMS Yeast Res. 2008 Jun; 8(4):586-96.FY

Abstract

In this work, we compared the mtDNA of a number of interspecific Saccharomyces hybrids (Saccharomyces cerevisiae x Saccharomyces uvarum and S. cerevisiae x Saccharomyces bayanus) to the mtDNA of 22 lager brewing strains that are thought to be the result of a natural hybridization between S. cerevisiae and another Saccharomyces yeast, possibly belonging to the species S. bayanus. We detected that in hybrids constructed in vitro, the mtDNA could be inherited from either parental strain. Conversely, in the lager strains tested, the mtDNA was never of the S. cerevisiae type. Moreover, the nucleotide sequence of lager brewing strains COXII gene was identical to S. bayanus strain NBRC 1948 COXII gene. MtDNA restriction analysis carried out with three enzymes confirmed this finding. However, restriction analysis with a fourth enzyme (AvaI) provided restriction patterns for lager strains that differed from those of S. bayanus strain NBRC 1948. Our results raise the hypothesis that the human-driven selection carried out on existing lager yeasts has favored only those bearing optimal fermentation characteristics at low temperatures, which harbor the mtDNA of S. bayanus.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Rainieri S
Department of Agricultural and Food Sciences, University of Modena and Reggio Emilia, Reggio Emilia, Italy. srainieri@suk.azti.es
Kodama Y
No affiliation info available
Nakao Y
No affiliation info available
Pulvirenti A
No affiliation info available
Giudici P
No affiliation info available

MeSH

Cluster AnalysisDNA FingerprintingDNA, FungalDNA, MitochondrialElectron Transport Complex IVEvolution, MolecularFungal ProteinsIndustrial MicrobiologyMolecular Sequence DataPolymorphism, Restriction Fragment LengthSaccharomycesSequence Analysis, DNA

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18318709

Citation

Rainieri, Sandra, et al. "The Inheritance of mtDNA in Lager Brewing Strains." FEMS Yeast Research, vol. 8, no. 4, 2008, pp. 586-96.
Rainieri S, Kodama Y, Nakao Y, et al. The inheritance of mtDNA in lager brewing strains. FEMS Yeast Res. 2008;8(4):586-96.
Rainieri, S., Kodama, Y., Nakao, Y., Pulvirenti, A., & Giudici, P. (2008). The inheritance of mtDNA in lager brewing strains. FEMS Yeast Research, 8(4), 586-96. https://doi.org/10.1111/j.1567-1364.2008.00363.x
Rainieri S, et al. The Inheritance of mtDNA in Lager Brewing Strains. FEMS Yeast Res. 2008;8(4):586-96. PubMed PMID: 18318709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The inheritance of mtDNA in lager brewing strains. AU - Rainieri,Sandra, AU - Kodama,Yukiko, AU - Nakao,Yoshihiro, AU - Pulvirenti,Andrea, AU - Giudici,Paolo, Y1 - 2008/03/03/ PY - 2008/3/6/pubmed PY - 2008/7/17/medline PY - 2008/3/6/entrez SP - 586 EP - 96 JF - FEMS yeast research JO - FEMS Yeast Res VL - 8 IS - 4 N2 - In this work, we compared the mtDNA of a number of interspecific Saccharomyces hybrids (Saccharomyces cerevisiae x Saccharomyces uvarum and S. cerevisiae x Saccharomyces bayanus) to the mtDNA of 22 lager brewing strains that are thought to be the result of a natural hybridization between S. cerevisiae and another Saccharomyces yeast, possibly belonging to the species S. bayanus. We detected that in hybrids constructed in vitro, the mtDNA could be inherited from either parental strain. Conversely, in the lager strains tested, the mtDNA was never of the S. cerevisiae type. Moreover, the nucleotide sequence of lager brewing strains COXII gene was identical to S. bayanus strain NBRC 1948 COXII gene. MtDNA restriction analysis carried out with three enzymes confirmed this finding. However, restriction analysis with a fourth enzyme (AvaI) provided restriction patterns for lager strains that differed from those of S. bayanus strain NBRC 1948. Our results raise the hypothesis that the human-driven selection carried out on existing lager yeasts has favored only those bearing optimal fermentation characteristics at low temperatures, which harbor the mtDNA of S. bayanus. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/18318709/The_inheritance_of_mtDNA_in_lager_brewing_strains_ DB - PRIME DP - Unbound Medicine ER -