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Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA.
Nature. 1991 Aug 29; 352(6338):818-21.Nat

Abstract

Splicing of pre-messenger RNA in eukaryotic cells occurs in a multicomponent complex termed the spliceosome, which contains small nuclear ribonucleoprotein particles (snRNPs), protein factors and substrate pre-mRNA. Assembly of the spliceosome involves the stepwise binding of snRNPs and protein factors to the pre-mRNA through a poorly understood mechanism which probably involves specific RNA-RNA, RNA-protein and protein-protein interactions. Of particular interest are the interactions between snRNPs, which are likely to be important not only for assembly of the spliceosome but also for catalysis. U1 snRNP interacts with the 5' splice site and U2 snRNP with the branch site of the pre-mRNA; both of these interactions involve Watson-Crick base pairing. But very little is known about how other factors such as the U4/U6 and U5 snRNPs reach the spliceosome and function in splicing. Here we report evidence that U6 snRNA interacts directly with U2 snRNA by a mechanism involving base-pairing, and that this interaction can be necessary for splicing of a mammalian pre-mRNA in vivo.

Authors+Show Affiliations

Department of Biological Sciences, Columbia University, New York, New York 10027.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1831878

Citation

Wu, J A., and J L. Manley. "Base Pairing Between U2 and U6 snRNAs Is Necessary for Splicing of a Mammalian Pre-mRNA." Nature, vol. 352, no. 6338, 1991, pp. 818-21.
Wu JA, Manley JL. Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA. Nature. 1991;352(6338):818-21.
Wu, J. A., & Manley, J. L. (1991). Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA. Nature, 352(6338), 818-21.
Wu JA, Manley JL. Base Pairing Between U2 and U6 snRNAs Is Necessary for Splicing of a Mammalian Pre-mRNA. Nature. 1991 Aug 29;352(6338):818-21. PubMed PMID: 1831878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA. AU - Wu,J A, AU - Manley,J L, PY - 1991/8/29/pubmed PY - 1991/8/29/medline PY - 1991/8/29/entrez SP - 818 EP - 21 JF - Nature JO - Nature VL - 352 IS - 6338 N2 - Splicing of pre-messenger RNA in eukaryotic cells occurs in a multicomponent complex termed the spliceosome, which contains small nuclear ribonucleoprotein particles (snRNPs), protein factors and substrate pre-mRNA. Assembly of the spliceosome involves the stepwise binding of snRNPs and protein factors to the pre-mRNA through a poorly understood mechanism which probably involves specific RNA-RNA, RNA-protein and protein-protein interactions. Of particular interest are the interactions between snRNPs, which are likely to be important not only for assembly of the spliceosome but also for catalysis. U1 snRNP interacts with the 5' splice site and U2 snRNP with the branch site of the pre-mRNA; both of these interactions involve Watson-Crick base pairing. But very little is known about how other factors such as the U4/U6 and U5 snRNPs reach the spliceosome and function in splicing. Here we report evidence that U6 snRNA interacts directly with U2 snRNA by a mechanism involving base-pairing, and that this interaction can be necessary for splicing of a mammalian pre-mRNA in vivo. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/1831878/Base_pairing_between_U2_and_U6_snRNAs_is_necessary_for_splicing_of_a_mammalian_pre_mRNA_ DB - PRIME DP - Unbound Medicine ER -