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Genetic evidence for base pairing between U2 and U6 snRNA in mammalian mRNA splicing.
Nature. 1991 Aug 29; 352(6338):821-4.Nat

Abstract

Removal of introns from eukaryotic nuclear messenger RNA precursors is catalysed by a large ribonucleoprotein complex called the spliceosome, which consists of four small nuclear ribonucleoprotein particles (U1, U2, U5, and U4/U6 snRNPs) and auxiliary protein factors. We have begun a genetic analysis of mammalian U2 snRNA by making second-site mutations in a suppressor U2 snRNA. Here we find that several mutations in the 5' end of U2 (nucleotides 3-8) are deleterious and that one of these can be rescued by compensatory base changes in the 3' end of U6 (nucleotides 92-95). The results demonstrate genetically that the base-pairing interaction between U2 (nucleotides 3-11) and U6 snRNA (nucleotides 87-95), originally proposed on the basis of psoralen photocrosslinking experiments, can influence the efficiency of mRNA splicing in mammals. The U2/U6 interaction in yeast, however, is fairly tolerant to mutation (D.J. Field and J.D. Friesen, personal communication), emphasizing the potential for facultative RNA interactions within the spliceosome.

Authors+Show Affiliations

Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06510.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1831879

Citation

Datta, B, and A M. Weiner. "Genetic Evidence for Base Pairing Between U2 and U6 snRNA in Mammalian mRNA Splicing." Nature, vol. 352, no. 6338, 1991, pp. 821-4.
Datta B, Weiner AM. Genetic evidence for base pairing between U2 and U6 snRNA in mammalian mRNA splicing. Nature. 1991;352(6338):821-4.
Datta, B., & Weiner, A. M. (1991). Genetic evidence for base pairing between U2 and U6 snRNA in mammalian mRNA splicing. Nature, 352(6338), 821-4.
Datta B, Weiner AM. Genetic Evidence for Base Pairing Between U2 and U6 snRNA in Mammalian mRNA Splicing. Nature. 1991 Aug 29;352(6338):821-4. PubMed PMID: 1831879.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic evidence for base pairing between U2 and U6 snRNA in mammalian mRNA splicing. AU - Datta,B, AU - Weiner,A M, PY - 1991/8/29/pubmed PY - 1991/8/29/medline PY - 1991/8/29/entrez SP - 821 EP - 4 JF - Nature JO - Nature VL - 352 IS - 6338 N2 - Removal of introns from eukaryotic nuclear messenger RNA precursors is catalysed by a large ribonucleoprotein complex called the spliceosome, which consists of four small nuclear ribonucleoprotein particles (U1, U2, U5, and U4/U6 snRNPs) and auxiliary protein factors. We have begun a genetic analysis of mammalian U2 snRNA by making second-site mutations in a suppressor U2 snRNA. Here we find that several mutations in the 5' end of U2 (nucleotides 3-8) are deleterious and that one of these can be rescued by compensatory base changes in the 3' end of U6 (nucleotides 92-95). The results demonstrate genetically that the base-pairing interaction between U2 (nucleotides 3-11) and U6 snRNA (nucleotides 87-95), originally proposed on the basis of psoralen photocrosslinking experiments, can influence the efficiency of mRNA splicing in mammals. The U2/U6 interaction in yeast, however, is fairly tolerant to mutation (D.J. Field and J.D. Friesen, personal communication), emphasizing the potential for facultative RNA interactions within the spliceosome. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/1831879/Genetic_evidence_for_base_pairing_between_U2_and_U6_snRNA_in_mammalian_mRNA_splicing_ L2 - https://doi.org/10.1038/352821a0 DB - PRIME DP - Unbound Medicine ER -