Tags

Type your tag names separated by a space and hit enter

Establishment and characterization of a cell based artificial antigen-presenting cell for expansion and activation of CD8+ T cells ex vivo.
Cell Mol Immunol. 2008 Feb; 5(1):47-53.CM

Abstract

Artificial antigen-presenting cells are expected to stimulate the expansion and acquisition of optimal therapeutic features of T cells before infusion. Here CD32 that binds to a crystallizable fragment of IgG monoclonal antibody was genetically expressed on human K562 leukemia cells to provide a ligand for T-cell receptor. CD86 and 4-1BBL, which are ligands of co-stimulating receptors of CD28 and 4-1BB, respectively, were also expressed on K562 cells. Then we accomplished the artificial antigen-presenting cells by coupling K32/CD86/4-1BBL cell with OKT3 monoclonal antibody against CD3, named K32/CD86/4-1BBL/OKT3 cells. These artificial modified cells had the abilities of inducing CD8+ T cell activation, promoting CD8+ T cell proliferation, division, and long-term growth, inhibiting CD8+ T cell apoptosis, and enhancing CD8+ T cell secretion of IFN-gamma and perforin. Furthermore, antigen-specific cytotoxic T lymphocytes could be retained in the culture stimulated with K32/CD86/4-1BBL/OKT3 cells at least within 28 days. This approach was robust, simple, reproducible and economical for expansion and activation of CD8+ T cells and may have important therapeutic implications for adoptive immunotherapy.

Authors+Show Affiliations

Department of Immunology, School of Medicine, Yangzhou University, Yangzhou 225001, China. gwj1974@263.netNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18318994

Citation

Gong, Weijuan, et al. "Establishment and Characterization of a Cell Based Artificial Antigen-presenting Cell for Expansion and Activation of CD8+ T Cells Ex Vivo." Cellular & Molecular Immunology, vol. 5, no. 1, 2008, pp. 47-53.
Gong W, Ji M, Cao Z, et al. Establishment and characterization of a cell based artificial antigen-presenting cell for expansion and activation of CD8+ T cells ex vivo. Cell Mol Immunol. 2008;5(1):47-53.
Gong, W., Ji, M., Cao, Z., Wang, L., Qian, Y., Hu, M., Qian, L., & Pan, X. (2008). Establishment and characterization of a cell based artificial antigen-presenting cell for expansion and activation of CD8+ T cells ex vivo. Cellular & Molecular Immunology, 5(1), 47-53. https://doi.org/10.1038/cmi.2008.6
Gong W, et al. Establishment and Characterization of a Cell Based Artificial Antigen-presenting Cell for Expansion and Activation of CD8+ T Cells Ex Vivo. Cell Mol Immunol. 2008;5(1):47-53. PubMed PMID: 18318994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Establishment and characterization of a cell based artificial antigen-presenting cell for expansion and activation of CD8+ T cells ex vivo. AU - Gong,Weijuan, AU - Ji,Mingchun, AU - Cao,Zhengfeng, AU - Wang,Liheng, AU - Qian,Yayun, AU - Hu,Maozhi, AU - Qian,Li, AU - Pan,Xingyuan, PY - 2008/3/6/pubmed PY - 2008/7/17/medline PY - 2008/3/6/entrez SP - 47 EP - 53 JF - Cellular & molecular immunology JO - Cell. Mol. Immunol. VL - 5 IS - 1 N2 - Artificial antigen-presenting cells are expected to stimulate the expansion and acquisition of optimal therapeutic features of T cells before infusion. Here CD32 that binds to a crystallizable fragment of IgG monoclonal antibody was genetically expressed on human K562 leukemia cells to provide a ligand for T-cell receptor. CD86 and 4-1BBL, which are ligands of co-stimulating receptors of CD28 and 4-1BB, respectively, were also expressed on K562 cells. Then we accomplished the artificial antigen-presenting cells by coupling K32/CD86/4-1BBL cell with OKT3 monoclonal antibody against CD3, named K32/CD86/4-1BBL/OKT3 cells. These artificial modified cells had the abilities of inducing CD8+ T cell activation, promoting CD8+ T cell proliferation, division, and long-term growth, inhibiting CD8+ T cell apoptosis, and enhancing CD8+ T cell secretion of IFN-gamma and perforin. Furthermore, antigen-specific cytotoxic T lymphocytes could be retained in the culture stimulated with K32/CD86/4-1BBL/OKT3 cells at least within 28 days. This approach was robust, simple, reproducible and economical for expansion and activation of CD8+ T cells and may have important therapeutic implications for adoptive immunotherapy. SN - 1672-7681 UR - https://www.unboundmedicine.com/medline/citation/18318994/Establishment_and_characterization_of_a_cell_based_artificial_antigen_presenting_cell_for_expansion_and_activation_of_CD8+_T_cells_ex_vivo_ L2 - http://www.cmi.ustc.edu.cn/5/1/47.pdf DB - PRIME DP - Unbound Medicine ER -