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Immunophenotypic characterization of the leukemic B-cells from Iranian patients with chronic lymphocytic leukemia: association between CD38 expression and disease progression.
Iran J Immunol. 2008 Mar; 5(1):25-35.IJ

Abstract

BACKGROUND

Patients with B-cell chronic lymphocytic leukemia (B-CLL) have heterogeneous clinical courses, thus several biological parameters need to be added to the current clinical staging systems to predict disease outcome. Recent immunophenotypic studies performed mainly in Western populations have demonstrated the prognostic value of CD38 and ZAP-70 expression in B-CLL.

OBJECTIVES

To investigate the expression pattern of a variety of membrane antigens on leukemic cells from Iranian patients with CLL and to find out if there are any differences in the expression of these markers between indolent and progressive groups.

METHODS

In the present study, peripheral blood samples from 87 Iranian patients with B-CLL were analysed by flow cytometry.

RESULTS

In all cases, the neoplastic cells displayed B-CLL phenotype (CD5+/CD19+/sIg+). The vast majority of the cases expressed CD23, but failed to stain for CD3 or CD14. The leukemic cells of most patients expressed CD27 (84/87, 95.4%) and CD45RO (74/87, 83.9%) molecules, suggesting a memory B-cell phenotype. Comparison between the indolent (n=42) and progressive (n=37) patients revealed significantly higher frequency and intensity of CD38 expression in progressive group (40.5%) compared to indolent (11.9%) patients (p<0.05). None of the other membrane antigens were differentially expressed in these two groups of patients.

CONCLUSION

Our results obtained in an Asian ethnic population confirm and extend previous findings obtained from Western populations regarding the association of CD38 expression and disease progression in B-CLL.

Authors+Show Affiliations

Department of Immunology, School of Public Health, Medical Sciences/Tehran University, Tehran, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18319522

Citation

Hojjat Farsangi, Mohammad, et al. "Immunophenotypic Characterization of the Leukemic B-cells From Iranian Patients With Chronic Lymphocytic Leukemia: Association Between CD38 Expression and Disease Progression." Iranian Journal of Immunology : IJI, vol. 5, no. 1, 2008, pp. 25-35.
Hojjat Farsangi M, Jeddi-Tehrani M, Razavi SM, et al. Immunophenotypic characterization of the leukemic B-cells from Iranian patients with chronic lymphocytic leukemia: association between CD38 expression and disease progression. Iran J Immunol. 2008;5(1):25-35.
Hojjat Farsangi, M., Jeddi-Tehrani, M., Razavi, S. M., Sharifian, R. A., Shamsian Khoramabadi, A., Rabbani, H., & Shokri, F. (2008). Immunophenotypic characterization of the leukemic B-cells from Iranian patients with chronic lymphocytic leukemia: association between CD38 expression and disease progression. Iranian Journal of Immunology : IJI, 5(1), 25-35. https://doi.org/IJIv5i1A2
Hojjat Farsangi M, et al. Immunophenotypic Characterization of the Leukemic B-cells From Iranian Patients With Chronic Lymphocytic Leukemia: Association Between CD38 Expression and Disease Progression. Iran J Immunol. 2008;5(1):25-35. PubMed PMID: 18319522.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunophenotypic characterization of the leukemic B-cells from Iranian patients with chronic lymphocytic leukemia: association between CD38 expression and disease progression. AU - Hojjat Farsangi,Mohammad, AU - Jeddi-Tehrani,Mahmood, AU - Razavi,Seyed Mohsen, AU - Sharifian,Ramazan Ali, AU - Shamsian Khoramabadi,Ahmad, AU - Rabbani,Hodjatallah, AU - Shokri,Fazel, PY - 2008/3/6/pubmed PY - 2008/8/30/medline PY - 2008/3/6/entrez SP - 25 EP - 35 JF - Iranian journal of immunology : IJI JO - Iran J Immunol VL - 5 IS - 1 N2 - BACKGROUND: Patients with B-cell chronic lymphocytic leukemia (B-CLL) have heterogeneous clinical courses, thus several biological parameters need to be added to the current clinical staging systems to predict disease outcome. Recent immunophenotypic studies performed mainly in Western populations have demonstrated the prognostic value of CD38 and ZAP-70 expression in B-CLL. OBJECTIVES: To investigate the expression pattern of a variety of membrane antigens on leukemic cells from Iranian patients with CLL and to find out if there are any differences in the expression of these markers between indolent and progressive groups. METHODS: In the present study, peripheral blood samples from 87 Iranian patients with B-CLL were analysed by flow cytometry. RESULTS: In all cases, the neoplastic cells displayed B-CLL phenotype (CD5+/CD19+/sIg+). The vast majority of the cases expressed CD23, but failed to stain for CD3 or CD14. The leukemic cells of most patients expressed CD27 (84/87, 95.4%) and CD45RO (74/87, 83.9%) molecules, suggesting a memory B-cell phenotype. Comparison between the indolent (n=42) and progressive (n=37) patients revealed significantly higher frequency and intensity of CD38 expression in progressive group (40.5%) compared to indolent (11.9%) patients (p<0.05). None of the other membrane antigens were differentially expressed in these two groups of patients. CONCLUSION: Our results obtained in an Asian ethnic population confirm and extend previous findings obtained from Western populations regarding the association of CD38 expression and disease progression in B-CLL. SN - 1735-1383 UR - https://www.unboundmedicine.com/medline/citation/18319522/Immunophenotypic_characterization_of_the_leukemic_B_cells_from_Iranian_patients_with_chronic_lymphocytic_leukemia:_association_between_CD38_expression_and_disease_progression_ DB - PRIME DP - Unbound Medicine ER -