Transient expression of an EP1-like gene encoded in Cotesia plutellae bracovirus suppresses the hemocyte population in the diamondback moth, Plutella xylostella.
Dev Comp Immunol. 2008; 32(8):932-42.DC

Abstract

A genome project has been launched and aims to sequence total genome of Cotesia plutellae bracovirus (CpBV). This on-going research has identified seven EP1-like (ELP) genes in the CpBV genome. A group of ELP genes has been speculated as an immunosuppressant encoded in Cotesia-associated bracoviruses. This study analyzed gene expression of these seven CpBV-ELPs in the parasitized diamondback moth, Plutella xylostella. Of these, six CpBV-ELPs were expressed in P. xylostella parasitized by C. plutellae. However, their expression levels varied in different tissues and parasitization stages. Especially, CpBV-ELP1 showed a persistent and ubiquitous expression pattern in both reverse transcriptase-polymerase chain reaction (RT-PCR) and immunofluorescence assays. When nonparasitized P. xylostella was transfected with a recombinant CpBV-ELP1 in a eukaryotic expression vector, CpBV-ELP1 was expressed for at least 3 days and the proteins were detectable in the cytoplasm of hemocytes. The transfected larvae showed significant reduction in total hemocyte numbers, compared with larvae injected with the cloning vector alone. Co-transfection with double-stranded RNA could knock down the expression of CpBV-ELP1 and prevented the reduction of the hemocyte population. This study demonstrates that CpBV-ELP1 plays a physiological role in suppressing host immune response presumably by its hemolytic activity during C. plutellae parasitization.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kwon B

Department of Bioresource Sciences, Andong National University, Andong, Republic of Korea.

Kim Y

No affiliation info available

MeSH

Amino Acid SequenceAnimalsComputational BiologyGlycoproteinsHemocytesImmediate-Early ProteinsImmune ToleranceMolecular Sequence DataMothsPolydnaviridaeSymbiosisViral ProteinsWasps

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18321572

Citation

Kwon, Bowon, and Yonggyun Kim. "Transient Expression of an EP1-like Gene Encoded in Cotesia Plutellae Bracovirus Suppresses the Hemocyte Population in the Diamondback Moth, Plutella Xylostella." Developmental and Comparative Immunology, vol. 32, no. 8, 2008, pp. 932-42.

Kwon B, Kim Y. Transient expression of an EP1-like gene encoded in Cotesia plutellae bracovirus suppresses the hemocyte population in the diamondback moth, Plutella xylostella. Dev Comp Immunol. 2008;32(8):932-42.

Kwon, B., & Kim, Y. (2008). Transient expression of an EP1-like gene encoded in Cotesia plutellae bracovirus suppresses the hemocyte population in the diamondback moth, Plutella xylostella. Developmental and Comparative Immunology, 32(8), 932-42. https://doi.org/10.1016/j.dci.2008.01.005

Kwon B, Kim Y. Transient Expression of an EP1-like Gene Encoded in Cotesia Plutellae Bracovirus Suppresses the Hemocyte Population in the Diamondback Moth, Plutella Xylostella. Dev Comp Immunol. 2008;32(8):932-42. PubMed PMID: 18321572.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Transient expression of an EP1-like gene encoded in Cotesia plutellae bracovirus suppresses the hemocyte population in the diamondback moth, Plutella xylostella. AU - Kwon,Bowon, AU - Kim,Yonggyun, Y1 - 2008/02/21/ PY - 2007/12/22/received PY - 2008/01/11/revised PY - 2008/01/14/accepted PY - 2008/3/7/pubmed PY - 2008/6/25/medline PY - 2008/3/7/entrez SP - 932 EP - 42 JF - Developmental and comparative immunology JO - Dev Comp Immunol VL - 32 IS - 8 N2 - A genome project has been launched and aims to sequence total genome of Cotesia plutellae bracovirus (CpBV). This on-going research has identified seven EP1-like (ELP) genes in the CpBV genome. A group of ELP genes has been speculated as an immunosuppressant encoded in Cotesia-associated bracoviruses. This study analyzed gene expression of these seven CpBV-ELPs in the parasitized diamondback moth, Plutella xylostella. Of these, six CpBV-ELPs were expressed in P. xylostella parasitized by C. plutellae. However, their expression levels varied in different tissues and parasitization stages. Especially, CpBV-ELP1 showed a persistent and ubiquitous expression pattern in both reverse transcriptase-polymerase chain reaction (RT-PCR) and immunofluorescence assays. When nonparasitized P. xylostella was transfected with a recombinant CpBV-ELP1 in a eukaryotic expression vector, CpBV-ELP1 was expressed for at least 3 days and the proteins were detectable in the cytoplasm of hemocytes. The transfected larvae showed significant reduction in total hemocyte numbers, compared with larvae injected with the cloning vector alone. Co-transfection with double-stranded RNA could knock down the expression of CpBV-ELP1 and prevented the reduction of the hemocyte population. This study demonstrates that CpBV-ELP1 plays a physiological role in suppressing host immune response presumably by its hemolytic activity during C. plutellae parasitization. SN - 0145-305X UR - https://www.unboundmedicine.com/medline/citation/18321572/Transient_expression_of_an_EP1_like_gene_encoded_in_Cotesia_plutellae_bracovirus_suppresses_the_hemocyte_population_in_the_diamondback_moth_Plutella_xylostella_ DB - PRIME DP - Unbound Medicine ER -

Tags

Transient expression of an EP1-like gene encoded in Cotesia plutellae bracovirus suppresses the hemocyte population in the diamondback moth, Plutella xylostella.Dev Comp Immunol. 2008; 32(8):932-42.DC