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Postprandial chylomicrons and adipose tissue lipoprotein lipase are altered in type 2 diabetes independently of obesity and whole-body insulin resistance.
Nutr Metab Cardiovasc Dis. 2008 Oct; 18(8):531-8.NM

Abstract

BACKGROUND AND AIMS

Postprandial lipoprotein abnormalities in type 2 diabetes are associated with insulin resistance. The role of other diabetes-related factors is still not clear. The aim of this study is to differentiate the effects of whole-body insulin resistance, obesity, and type 2 diabetes on postprandial dyslipidaemia and lipoprotein lipase (LPL) in adipose tissue.

METHODS AND RESULTS

Ten subjects with obesity and diabetes (OD), 11 with obesity alone (O), and 11 normal-weight controls (C) - males, aged 26-59 years, with fasting normo-triglyceridaemia underwent measurements of cholesterol, triglycerides, apo B-48 and apo B-100 concentrations in plasma lipoproteins separated by density gradient ultracentrifugation before and after a fat-rich meal. Fasting and postprandial (6h) LPL activity was determined in abdominal subcutaneous adipose tissue biopsy samples. Insulin sensitivity was measured by hyperinsulinaemic euglycaemic clamp. OD and O subjects had similar degrees of adiposity (BMI, waist circumference, fat mass) and insulin resistance (insulin stimulated glucose disposal and M/I). They also showed a similarly higher postprandial increase in large VLDL lipids (triglyceride incremental AUC 188+/-28 and 135+/-22 mg/dl.6h) than C (87+/-13 mg/dl.6h, M+/-SEM, p<0.05). OD had an increased chylomicron response compared to O (triglyceride incremental AUC 132+/-23 vs. 75+/-14 mg/dl.6h, p<0.05). OD had significantly lower fasting and postprandial adipose tissue heparin-releasable LPL activity than O and C.

CONCLUSIONS

In insulin-resistant conditions of obesity, with and without diabetes, large VLDL are increased after a fat-rich meal. In addition, diabetic patients compared to obese subjects have an increased postprandial chylomicron response and a reduced adipose tissue LPL activity.

Authors+Show Affiliations

Department of Clinical and Experimental Medicine, Federico II University, Via Pansini 5, 80131 Naples, Italy. annuzzi@unina.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18321693

Citation

Annuzzi, Giovanni, et al. "Postprandial Chylomicrons and Adipose Tissue Lipoprotein Lipase Are Altered in Type 2 Diabetes Independently of Obesity and Whole-body Insulin Resistance." Nutrition, Metabolism, and Cardiovascular Diseases : NMCD, vol. 18, no. 8, 2008, pp. 531-8.
Annuzzi G, Giacco R, Patti L, et al. Postprandial chylomicrons and adipose tissue lipoprotein lipase are altered in type 2 diabetes independently of obesity and whole-body insulin resistance. Nutr Metab Cardiovasc Dis. 2008;18(8):531-8.
Annuzzi, G., Giacco, R., Patti, L., Di Marino, L., De Natale, C., Costabile, G., Marra, M., Santangelo, C., Masella, R., & Rivellese, A. A. (2008). Postprandial chylomicrons and adipose tissue lipoprotein lipase are altered in type 2 diabetes independently of obesity and whole-body insulin resistance. Nutrition, Metabolism, and Cardiovascular Diseases : NMCD, 18(8), 531-8. https://doi.org/10.1016/j.numecd.2007.12.003
Annuzzi G, et al. Postprandial Chylomicrons and Adipose Tissue Lipoprotein Lipase Are Altered in Type 2 Diabetes Independently of Obesity and Whole-body Insulin Resistance. Nutr Metab Cardiovasc Dis. 2008;18(8):531-8. PubMed PMID: 18321693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Postprandial chylomicrons and adipose tissue lipoprotein lipase are altered in type 2 diabetes independently of obesity and whole-body insulin resistance. AU - Annuzzi,Giovanni, AU - Giacco,Rosalba, AU - Patti,Lidia, AU - Di Marino,Lucrezia, AU - De Natale,Claudia, AU - Costabile,Giuseppina, AU - Marra,Maurizio, AU - Santangelo,Carmela, AU - Masella,Roberta, AU - Rivellese,Angela A, Y1 - 2008/03/05/ PY - 2007/09/07/received PY - 2007/11/15/revised PY - 2007/12/05/accepted PY - 2008/3/7/pubmed PY - 2009/2/14/medline PY - 2008/3/7/entrez SP - 531 EP - 8 JF - Nutrition, metabolism, and cardiovascular diseases : NMCD JO - Nutr Metab Cardiovasc Dis VL - 18 IS - 8 N2 - BACKGROUND AND AIMS: Postprandial lipoprotein abnormalities in type 2 diabetes are associated with insulin resistance. The role of other diabetes-related factors is still not clear. The aim of this study is to differentiate the effects of whole-body insulin resistance, obesity, and type 2 diabetes on postprandial dyslipidaemia and lipoprotein lipase (LPL) in adipose tissue. METHODS AND RESULTS: Ten subjects with obesity and diabetes (OD), 11 with obesity alone (O), and 11 normal-weight controls (C) - males, aged 26-59 years, with fasting normo-triglyceridaemia underwent measurements of cholesterol, triglycerides, apo B-48 and apo B-100 concentrations in plasma lipoproteins separated by density gradient ultracentrifugation before and after a fat-rich meal. Fasting and postprandial (6h) LPL activity was determined in abdominal subcutaneous adipose tissue biopsy samples. Insulin sensitivity was measured by hyperinsulinaemic euglycaemic clamp. OD and O subjects had similar degrees of adiposity (BMI, waist circumference, fat mass) and insulin resistance (insulin stimulated glucose disposal and M/I). They also showed a similarly higher postprandial increase in large VLDL lipids (triglyceride incremental AUC 188+/-28 and 135+/-22 mg/dl.6h) than C (87+/-13 mg/dl.6h, M+/-SEM, p<0.05). OD had an increased chylomicron response compared to O (triglyceride incremental AUC 132+/-23 vs. 75+/-14 mg/dl.6h, p<0.05). OD had significantly lower fasting and postprandial adipose tissue heparin-releasable LPL activity than O and C. CONCLUSIONS: In insulin-resistant conditions of obesity, with and without diabetes, large VLDL are increased after a fat-rich meal. In addition, diabetic patients compared to obese subjects have an increased postprandial chylomicron response and a reduced adipose tissue LPL activity. SN - 1590-3729 UR - https://www.unboundmedicine.com/medline/citation/18321693/Postprandial_chylomicrons_and_adipose_tissue_lipoprotein_lipase_are_altered_in_type_2_diabetes_independently_of_obesity_and_whole_body_insulin_resistance_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0939-4753(07)00224-4 DB - PRIME DP - Unbound Medicine ER -