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Effect of prostaglandin E2 and tumor necrosis factor alpha on the VEGF-receptor system expression in cultured porcine luteal cells.
Mol Reprod Dev. 2008 Oct; 75(10):1558-66.MR

Abstract

The purpose of the present study was to investigate the effects of prostaglandin (PG) E(2) and tumor necrosis factor (TNF) alpha on expression of vascular endothelial growth factor (VEGF) and its receptors, fms-like tyrosine kinase (Flt-1) and fetal liver kinase-1/kinase insert domain-containing receptor (Flk-1/KDR), in cultured porcine luteal cells. Real-time PCR was used for quantification of VEGF and its receptors mRNA, whereas VEGF release by luteal cells was determined by radioimmunoassay (RIA). Only the highest dose of PGE(2) (1 microM) after 6 hr of incubation stimulated VEGF release by luteal cells collected in the mid-luteal phase (P < 0.05). Moreover, PGE(2) (100 nM, 1 microM) significantly stimulated VEGF secretion by luteal cells in the late phase and during pregnancy on Days 10-12 (P < 0.05). Elevated mRNA expression of both VEGF 120 and VEGF 164 isoforms was found in luteal cells cultured with PGE(2). The lack of an effect of PGE(2) on VEGF receptors mRNA expression was observed. TNFalpha was able to significantly stimulate VEGF release from cells obtained in the mid- and late luteal phase or during early pregnancy. All tested doses enhanced mRNA levels of VEGF 120 isoform, but not VEGF 164. Additionally, TNFalpha was able to decrease Flk-1/KDR mRNA expression, whereas Flt-1 mRNA levels were not affected. These results indicated that PGE(2) and TNFalpha influenced VEGF ligand-receptor system expression in porcine luteal cells and may therefore play an important role in regulation of luteal functions during the estrous cycle and pregnancy in pigs.

Authors+Show Affiliations

Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18324670

Citation

Kowalczyk, Anna E., et al. "Effect of Prostaglandin E2 and Tumor Necrosis Factor Alpha On the VEGF-receptor System Expression in Cultured Porcine Luteal Cells." Molecular Reproduction and Development, vol. 75, no. 10, 2008, pp. 1558-66.
Kowalczyk AE, Kaczmarek MM, Schams D, et al. Effect of prostaglandin E2 and tumor necrosis factor alpha on the VEGF-receptor system expression in cultured porcine luteal cells. Mol Reprod Dev. 2008;75(10):1558-66.
Kowalczyk, A. E., Kaczmarek, M. M., Schams, D., & Ziecik, A. J. (2008). Effect of prostaglandin E2 and tumor necrosis factor alpha on the VEGF-receptor system expression in cultured porcine luteal cells. Molecular Reproduction and Development, 75(10), 1558-66. https://doi.org/10.1002/mrd.20897
Kowalczyk AE, et al. Effect of Prostaglandin E2 and Tumor Necrosis Factor Alpha On the VEGF-receptor System Expression in Cultured Porcine Luteal Cells. Mol Reprod Dev. 2008;75(10):1558-66. PubMed PMID: 18324670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of prostaglandin E2 and tumor necrosis factor alpha on the VEGF-receptor system expression in cultured porcine luteal cells. AU - Kowalczyk,Anna E, AU - Kaczmarek,Monika M, AU - Schams,Dieter, AU - Ziecik,Adam J, PY - 2008/3/8/pubmed PY - 2008/12/17/medline PY - 2008/3/8/entrez SP - 1558 EP - 66 JF - Molecular reproduction and development JO - Mol Reprod Dev VL - 75 IS - 10 N2 - The purpose of the present study was to investigate the effects of prostaglandin (PG) E(2) and tumor necrosis factor (TNF) alpha on expression of vascular endothelial growth factor (VEGF) and its receptors, fms-like tyrosine kinase (Flt-1) and fetal liver kinase-1/kinase insert domain-containing receptor (Flk-1/KDR), in cultured porcine luteal cells. Real-time PCR was used for quantification of VEGF and its receptors mRNA, whereas VEGF release by luteal cells was determined by radioimmunoassay (RIA). Only the highest dose of PGE(2) (1 microM) after 6 hr of incubation stimulated VEGF release by luteal cells collected in the mid-luteal phase (P < 0.05). Moreover, PGE(2) (100 nM, 1 microM) significantly stimulated VEGF secretion by luteal cells in the late phase and during pregnancy on Days 10-12 (P < 0.05). Elevated mRNA expression of both VEGF 120 and VEGF 164 isoforms was found in luteal cells cultured with PGE(2). The lack of an effect of PGE(2) on VEGF receptors mRNA expression was observed. TNFalpha was able to significantly stimulate VEGF release from cells obtained in the mid- and late luteal phase or during early pregnancy. All tested doses enhanced mRNA levels of VEGF 120 isoform, but not VEGF 164. Additionally, TNFalpha was able to decrease Flk-1/KDR mRNA expression, whereas Flt-1 mRNA levels were not affected. These results indicated that PGE(2) and TNFalpha influenced VEGF ligand-receptor system expression in porcine luteal cells and may therefore play an important role in regulation of luteal functions during the estrous cycle and pregnancy in pigs. SN - 1098-2795 UR - https://www.unboundmedicine.com/medline/citation/18324670/Effect_of_prostaglandin_E2_and_tumor_necrosis_factor_alpha_on_the_VEGF_receptor_system_expression_in_cultured_porcine_luteal_cells_ L2 - https://doi.org/10.1002/mrd.20897 DB - PRIME DP - Unbound Medicine ER -