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Modified silver nanoparticle as a hydrophobic affinity probe for analysis of peptides and proteins in biological samples by using liquid-liquid microextraction coupled to AP-MALDI-ion trap and MALDI-TOF mass spectrometry.
Anal Chem. 2008 Apr 01; 80(7):2583-9.AC

Abstract

A new approach of using modified silver nanoparticles (AgNPs) in toluene as hydrophobic affinity probes for the separation and preconcentration of peptides and proteins in biological samples prior to atmospheric pressure-matrix assisted laser desorption/ionization (AP-MALDI) ion trap mass spectrometry and matrix assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry has been successfully demonstrated. To our best knowledge, for the first time, the modified AgNPs with hydrophobic ligands, such as dodecanethiol (DT) and octadecanethiol (OT) in toluene, were used for the liquid-liquid microextraction (LLME) of peptides and proteins through the hydrophobic interactions. In the present investigation, gramicidin was chosen as a model compound to assess the hydrophobic extraction with the modified AgNPs. The optimum extraction efficiency of gramicidine was observed at pH 7.0 for 1.5 h of extraction time with 7% addition of salt. Compared to the conventional use of AP-MALDI-MS, a 266-388-fold improvement in the limit of detection (LOD) for gramicidin was obtained in urine and plasma samples. The lowest concentration of gramicidin that was detected by using modified AgNPs in urine and plasma samples was 0.13 and 0.16 microM, respectively. Furthermore, the proposed method was demonstrated for the extraction of other long chain proteins, like myoglobin, ubiquitin, and bovine serum albumin, in a sample solution by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The major feature of the newly synthesized modified AgNPs was that the target species could be efficiently separated and preconcentrated without sample loss prior to MALDI-MS detection for the sensitive and effective analysis of peptides and proteins in biological samples.

Authors+Show Affiliations

Department of Chemistry, National Sun Yat-Sen University, Taiwan, and National Sun Yat-sen University-Kaohsiung Medical University Joint Research Center, Kaohsiung, Taiwan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18324794

Citation

Shrivas, Kamlesh, and Hui-Fen Wu. "Modified Silver Nanoparticle as a Hydrophobic Affinity Probe for Analysis of Peptides and Proteins in Biological Samples By Using Liquid-liquid Microextraction Coupled to AP-MALDI-ion Trap and MALDI-TOF Mass Spectrometry." Analytical Chemistry, vol. 80, no. 7, 2008, pp. 2583-9.
Shrivas K, Wu HF. Modified silver nanoparticle as a hydrophobic affinity probe for analysis of peptides and proteins in biological samples by using liquid-liquid microextraction coupled to AP-MALDI-ion trap and MALDI-TOF mass spectrometry. Anal Chem. 2008;80(7):2583-9.
Shrivas, K., & Wu, H. F. (2008). Modified silver nanoparticle as a hydrophobic affinity probe for analysis of peptides and proteins in biological samples by using liquid-liquid microextraction coupled to AP-MALDI-ion trap and MALDI-TOF mass spectrometry. Analytical Chemistry, 80(7), 2583-9. https://doi.org/10.1021/ac702309w
Shrivas K, Wu HF. Modified Silver Nanoparticle as a Hydrophobic Affinity Probe for Analysis of Peptides and Proteins in Biological Samples By Using Liquid-liquid Microextraction Coupled to AP-MALDI-ion Trap and MALDI-TOF Mass Spectrometry. Anal Chem. 2008 Apr 1;80(7):2583-9. PubMed PMID: 18324794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modified silver nanoparticle as a hydrophobic affinity probe for analysis of peptides and proteins in biological samples by using liquid-liquid microextraction coupled to AP-MALDI-ion trap and MALDI-TOF mass spectrometry. AU - Shrivas,Kamlesh, AU - Wu,Hui-Fen, Y1 - 2008/03/07/ PY - 2008/3/8/pubmed PY - 2008/6/3/medline PY - 2008/3/8/entrez SP - 2583 EP - 9 JF - Analytical chemistry JO - Anal Chem VL - 80 IS - 7 N2 - A new approach of using modified silver nanoparticles (AgNPs) in toluene as hydrophobic affinity probes for the separation and preconcentration of peptides and proteins in biological samples prior to atmospheric pressure-matrix assisted laser desorption/ionization (AP-MALDI) ion trap mass spectrometry and matrix assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry has been successfully demonstrated. To our best knowledge, for the first time, the modified AgNPs with hydrophobic ligands, such as dodecanethiol (DT) and octadecanethiol (OT) in toluene, were used for the liquid-liquid microextraction (LLME) of peptides and proteins through the hydrophobic interactions. In the present investigation, gramicidin was chosen as a model compound to assess the hydrophobic extraction with the modified AgNPs. The optimum extraction efficiency of gramicidine was observed at pH 7.0 for 1.5 h of extraction time with 7% addition of salt. Compared to the conventional use of AP-MALDI-MS, a 266-388-fold improvement in the limit of detection (LOD) for gramicidin was obtained in urine and plasma samples. The lowest concentration of gramicidin that was detected by using modified AgNPs in urine and plasma samples was 0.13 and 0.16 microM, respectively. Furthermore, the proposed method was demonstrated for the extraction of other long chain proteins, like myoglobin, ubiquitin, and bovine serum albumin, in a sample solution by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The major feature of the newly synthesized modified AgNPs was that the target species could be efficiently separated and preconcentrated without sample loss prior to MALDI-MS detection for the sensitive and effective analysis of peptides and proteins in biological samples. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/18324794/Modified_silver_nanoparticle_as_a_hydrophobic_affinity_probe_for_analysis_of_peptides_and_proteins_in_biological_samples_by_using_liquid_liquid_microextraction_coupled_to_AP_MALDI_ion_trap_and_MALDI_TOF_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -