TY - JOUR T1 - Preparation and evaluation of solid-phase microextraction fiber based on molecularly imprinted polymers for trace analysis of tetracyclines in complicated samples. AU - Hu,Xiaogang, AU - Pan,Jialiang, AU - Hu,Yuling, AU - Huo,Yin, AU - Li,Gongke, Y1 - 2008/03/05/ PY - 2007/12/07/received PY - 2008/02/11/revised PY - 2008/02/15/accepted PY - 2008/3/8/pubmed PY - 2008/7/9/medline PY - 2008/3/8/entrez SP - 97 EP - 107 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1188 IS - 2 N2 - Molecularly imprinted polymer (MIP) is widely used in many fields because of its characteristics of high selectivity, chemical stability and easy preparation. To enhance the selectivity and applicability of solid-phase microextraction (SPME), a novel MIP-coated SPME fiber was firstly prepared by multiple co-polymerization method with tetracycline as template. It could be coupled directly to high-performance liquid chromatography (HPLC) and used for trace analysis of tetracyclines (TCs) in complicated samples. The characteristics and application of the fibers were investigated. The electron microscope provided a crosslinked and porous surface, and the average thickness of the MIP coating was 19.5 microm. Compared with the non-imprinted polymer (NIP) coated fibers, the special selectivity to tetracycline and structure-similar oxytetracycline, doxycycline, chlortetracycline were discovered with the MIP-coated fibers. The adsorption and desorption of TCs with the MIP-coated fiber could be achieved quickly. A method for the fluorimetric determination of four TCs by the MIP-coated SPME coupled with HPLC was developed. The optimized extraction conditions such as extraction solvent, desorption solvent, and stirring speed were studied. Linear ranges for the four TCs were 5.00-200 microg/L and detection limits were within the range of 1.0-2.3 microg/L. The method was applied to simultaneous multi-residue analysis of four TCs in the spiked chicken feed, chicken muscle, and milk samples with the satisfactory recoveries. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18325526/Preparation_and_evaluation_of_solid_phase_microextraction_fiber_based_on_molecularly_imprinted_polymers_for_trace_analysis_of_tetracyclines_in_complicated_samples_ DB - PRIME DP - Unbound Medicine ER -