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Altered endothelin receptor binding in response to nitric oxide synthase inhibition in the pregnant rat.
Reprod Sci. 2008 Apr; 15(4):366-73.RS

Abstract

The authors evaluate the expression of endothelin-1 (ET-1) and its receptors in the uterus and placenta during maternal nitric oxide synthase (NOS) inhibition. Timed-pregnant rats received L-NAME (2.5 mg/kg/h) or saline from day 14 to 21 of gestation. Uterine and placental tissues collected on day 21 were assayed for preproET-1, ET(A), and ET(B) mRNA expression; localization and expression of ET-1 and receptor proteins; and receptor activity. NOS inhibition did not affect preproET-1 mRNA expression in the placenta or uterus. ET(A) expression decreased in the uterine free wall, but no other changes in receptor mRNA expression were observed in the uterus or placenta. ET-1 and receptor proteins were unchanged. Placental ET(A) and ET(B) receptor binding decreased. Uterine ET(A) receptor binding decreased in the placental bed. ET-1, a prominent mediator during NOS inhibition, is not of uterine or placental origin. Reduced receptor binding activity is the primary means by which these tissues regulate their response to ET-1 in the setting of NOS inhibition.

Authors+Show Affiliations

Department of Obstetrics, Evanston Northwestern Healthcare, Evanston, Illinois, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

18325929

Citation

Neerhof, Mark G., et al. "Altered Endothelin Receptor Binding in Response to Nitric Oxide Synthase Inhibition in the Pregnant Rat." Reproductive Sciences (Thousand Oaks, Calif.), vol. 15, no. 4, 2008, pp. 366-73.
Neerhof MG, Jilling T, Synowiec S, et al. Altered endothelin receptor binding in response to nitric oxide synthase inhibition in the pregnant rat. Reprod Sci. 2008;15(4):366-73.
Neerhof, M. G., Jilling, T., Synowiec, S., Khan, S., & Thaete, L. G. (2008). Altered endothelin receptor binding in response to nitric oxide synthase inhibition in the pregnant rat. Reproductive Sciences (Thousand Oaks, Calif.), 15(4), 366-73. https://doi.org/10.1177/1933719107312627
Neerhof MG, et al. Altered Endothelin Receptor Binding in Response to Nitric Oxide Synthase Inhibition in the Pregnant Rat. Reprod Sci. 2008;15(4):366-73. PubMed PMID: 18325929.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Altered endothelin receptor binding in response to nitric oxide synthase inhibition in the pregnant rat. AU - Neerhof,Mark G, AU - Jilling,Tamas, AU - Synowiec,Sylvia, AU - Khan,Saira, AU - Thaete,Larry G, Y1 - 2008/03/06/ PY - 2008/3/8/pubmed PY - 2008/7/4/medline PY - 2008/3/8/entrez SP - 366 EP - 73 JF - Reproductive sciences (Thousand Oaks, Calif.) JO - Reprod Sci VL - 15 IS - 4 N2 - The authors evaluate the expression of endothelin-1 (ET-1) and its receptors in the uterus and placenta during maternal nitric oxide synthase (NOS) inhibition. Timed-pregnant rats received L-NAME (2.5 mg/kg/h) or saline from day 14 to 21 of gestation. Uterine and placental tissues collected on day 21 were assayed for preproET-1, ET(A), and ET(B) mRNA expression; localization and expression of ET-1 and receptor proteins; and receptor activity. NOS inhibition did not affect preproET-1 mRNA expression in the placenta or uterus. ET(A) expression decreased in the uterine free wall, but no other changes in receptor mRNA expression were observed in the uterus or placenta. ET-1 and receptor proteins were unchanged. Placental ET(A) and ET(B) receptor binding decreased. Uterine ET(A) receptor binding decreased in the placental bed. ET-1, a prominent mediator during NOS inhibition, is not of uterine or placental origin. Reduced receptor binding activity is the primary means by which these tissues regulate their response to ET-1 in the setting of NOS inhibition. SN - 1933-7205 UR - https://www.unboundmedicine.com/medline/citation/18325929/Altered_endothelin_receptor_binding_in_response_to_nitric_oxide_synthase_inhibition_in_the_pregnant_rat_ L2 - https://doi.org/10.1177/1933719107312627 DB - PRIME DP - Unbound Medicine ER -