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Modulation of antioxidant defences in digestive gland of Perna viridis (L.), on mercury exposures.
Chemosphere. 2008 May; 71(10):1977-85.C

Abstract

Sub-lethal effects of mercury exposure (110th of LC(50), i.e. 0.045 mg l(-1)) for 5, 10 and 15 d was investigated on oxidative stress parameters and antioxidant defences in digestive gland of Perna viridis. In addition to this an in vitro effect of mercury single and supplemented with reduced glutathione on lipid peroxidation was studied. Increased lipid peroxidation (during first 10 days and also during in vitro exposures), protein carbonyl and hydrogen peroxides (from 5th till last day of exposure) indicate the resultant oxidative stress in the mercury exposed specimen. DNA damage (F-value) response although less distinct on 5th and 15th d, its low values on 10th d and significant correlation with hydrogen peroxide suggests the toxic role of free radicals towards DNA integrity. Superoxide dismutase, which remains low initially (5th d) and increases later suggests its immediate response against superoxide radical. Higher activities of catalase, glutathione peroxidase and glutathione reductase on 15th d and glutathione-S-tranferase from 10th d onwards suggests the adaptive behaviour of the tissue against oxyradicals. Increasing levels of non-enzymatic antioxidant molecules, such as reduced glutathione and ascorbic acid indicated its involvement in counteracting oxidative damage. Further role of reduced glutathione in reducing Hg toxicity is evident in in vitro experiments where lipid peroxidation remains low in mercury concentrations supplemented with reduced glutathione. The elevated levels of metallothionein from 5th to 10th d suggest involvement of this protein in detoxification of reactive oxygen species and toxic metal. The above results suggest that both enzymatic and non-enzymatic antioxidants play an important role in protecting cell against Hg toxicity, which can be used as a biomarker of metal contamination in aquatic environment.

Authors+Show Affiliations

Environmental Laboratory, Biological Oceanography Division, National Institute of Oceanography, Dona Paula, Goa 403004, India. verlecar@nio.orgNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18329067

Citation

Verlecar, X N., et al. "Modulation of Antioxidant Defences in Digestive Gland of Perna Viridis (L.), On Mercury Exposures." Chemosphere, vol. 71, no. 10, 2008, pp. 1977-85.
Verlecar XN, Jena KB, Chainy GB. Modulation of antioxidant defences in digestive gland of Perna viridis (L.), on mercury exposures. Chemosphere. 2008;71(10):1977-85.
Verlecar, X. N., Jena, K. B., & Chainy, G. B. (2008). Modulation of antioxidant defences in digestive gland of Perna viridis (L.), on mercury exposures. Chemosphere, 71(10), 1977-85. https://doi.org/10.1016/j.chemosphere.2007.12.014
Verlecar XN, Jena KB, Chainy GB. Modulation of Antioxidant Defences in Digestive Gland of Perna Viridis (L.), On Mercury Exposures. Chemosphere. 2008;71(10):1977-85. PubMed PMID: 18329067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of antioxidant defences in digestive gland of Perna viridis (L.), on mercury exposures. AU - Verlecar,X N, AU - Jena,K B, AU - Chainy,G B N, Y1 - 2008/03/07/ PY - 2007/06/15/received PY - 2007/11/30/revised PY - 2007/12/14/accepted PY - 2008/3/11/pubmed PY - 2008/9/10/medline PY - 2008/3/11/entrez SP - 1977 EP - 85 JF - Chemosphere JO - Chemosphere VL - 71 IS - 10 N2 - Sub-lethal effects of mercury exposure (110th of LC(50), i.e. 0.045 mg l(-1)) for 5, 10 and 15 d was investigated on oxidative stress parameters and antioxidant defences in digestive gland of Perna viridis. In addition to this an in vitro effect of mercury single and supplemented with reduced glutathione on lipid peroxidation was studied. Increased lipid peroxidation (during first 10 days and also during in vitro exposures), protein carbonyl and hydrogen peroxides (from 5th till last day of exposure) indicate the resultant oxidative stress in the mercury exposed specimen. DNA damage (F-value) response although less distinct on 5th and 15th d, its low values on 10th d and significant correlation with hydrogen peroxide suggests the toxic role of free radicals towards DNA integrity. Superoxide dismutase, which remains low initially (5th d) and increases later suggests its immediate response against superoxide radical. Higher activities of catalase, glutathione peroxidase and glutathione reductase on 15th d and glutathione-S-tranferase from 10th d onwards suggests the adaptive behaviour of the tissue against oxyradicals. Increasing levels of non-enzymatic antioxidant molecules, such as reduced glutathione and ascorbic acid indicated its involvement in counteracting oxidative damage. Further role of reduced glutathione in reducing Hg toxicity is evident in in vitro experiments where lipid peroxidation remains low in mercury concentrations supplemented with reduced glutathione. The elevated levels of metallothionein from 5th to 10th d suggest involvement of this protein in detoxification of reactive oxygen species and toxic metal. The above results suggest that both enzymatic and non-enzymatic antioxidants play an important role in protecting cell against Hg toxicity, which can be used as a biomarker of metal contamination in aquatic environment. SN - 0045-6535 UR - https://www.unboundmedicine.com/medline/citation/18329067/Modulation_of_antioxidant_defences_in_digestive_gland_of_Perna_viridis__L___on_mercury_exposures_ DB - PRIME DP - Unbound Medicine ER -