Tags

Type your tag names separated by a space and hit enter

Increased GFR and renal excretory function by activation of TRPV1 in the isolated perfused kidney.
Pharmacol Res. 2008 Mar; 57(3):239-46.PR

Abstract

To test the hypothesis that activation of the transient receptor potential vanilloid type 1 (TRPV1) channels leads to natriuresis and diuresis via an increase in glomerular filtration rate (GFR), recirculating Krebs-Henseleit buffer added with inulin was perfused in the isolated perfused kidney of male Wistar rat at a constant flow, and perfusion pressures (PPs) were pre-adjusted to three different levels (approximately 100, approximately 150, and approximately 190mmHg) with phenylephrine. Capsaicin (Cap), a selective TRPV1 agonist, was perfused in the presence or absence of capsazepine (Capz), a selective TRPV1 antagonist, CGRP(8-37), a selective calcitonin gene-related peptide (CGRP) receptor antagonist, or spantide II (Spa), a selective substance P (SP) receptor antagonist. At the higher (150 and 190mmHg) but not baseline (100mmHg) PP levels, Cap at 10microM significantly decreased PP and increased GFR, urine flow rate (UFR) and Na+ excretion (UNaV). At the highest (190mmHg) PP level, Cap (2, 10, 30microM) dose-dependently decreased PP and increased GFR, UFR, UNaV, and the release of CGRP and SP. Capz or CGRP(8-37) combined with Spa fully blocked the effect of Cap on PP, GFR, UFR, UNaV, and the release of CGRP and SP. In conclusion, activation of TRPV1 in the isolated kidney decreases renal PP and increases GFR and water/sodium excretion possibly via simultaneous activation of CGRP and SP receptors upon their enhanced release, suggesting that TRPV1 plays a key role in modulating renal hemodynamics and excretory function.

Authors+Show Affiliations

Department of Medicine, Neuroscience Program, and Cell and Molecular Biology Program, Michigan State University, East Lansing, MI 48824, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18329285

Citation

Li, Jianping, and Donna H. Wang. "Increased GFR and Renal Excretory Function By Activation of TRPV1 in the Isolated Perfused Kidney." Pharmacological Research, vol. 57, no. 3, 2008, pp. 239-46.
Li J, Wang DH. Increased GFR and renal excretory function by activation of TRPV1 in the isolated perfused kidney. Pharmacol Res. 2008;57(3):239-46.
Li, J., & Wang, D. H. (2008). Increased GFR and renal excretory function by activation of TRPV1 in the isolated perfused kidney. Pharmacological Research, 57(3), 239-46. https://doi.org/10.1016/j.phrs.2008.01.011
Li J, Wang DH. Increased GFR and Renal Excretory Function By Activation of TRPV1 in the Isolated Perfused Kidney. Pharmacol Res. 2008;57(3):239-46. PubMed PMID: 18329285.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased GFR and renal excretory function by activation of TRPV1 in the isolated perfused kidney. AU - Li,Jianping, AU - Wang,Donna H, Y1 - 2008/02/02/ PY - 2007/12/14/received PY - 2008/01/15/revised PY - 2008/01/29/accepted PY - 2008/3/11/pubmed PY - 2008/7/19/medline PY - 2008/3/11/entrez SP - 239 EP - 46 JF - Pharmacological research JO - Pharmacol Res VL - 57 IS - 3 N2 - To test the hypothesis that activation of the transient receptor potential vanilloid type 1 (TRPV1) channels leads to natriuresis and diuresis via an increase in glomerular filtration rate (GFR), recirculating Krebs-Henseleit buffer added with inulin was perfused in the isolated perfused kidney of male Wistar rat at a constant flow, and perfusion pressures (PPs) were pre-adjusted to three different levels (approximately 100, approximately 150, and approximately 190mmHg) with phenylephrine. Capsaicin (Cap), a selective TRPV1 agonist, was perfused in the presence or absence of capsazepine (Capz), a selective TRPV1 antagonist, CGRP(8-37), a selective calcitonin gene-related peptide (CGRP) receptor antagonist, or spantide II (Spa), a selective substance P (SP) receptor antagonist. At the higher (150 and 190mmHg) but not baseline (100mmHg) PP levels, Cap at 10microM significantly decreased PP and increased GFR, urine flow rate (UFR) and Na+ excretion (UNaV). At the highest (190mmHg) PP level, Cap (2, 10, 30microM) dose-dependently decreased PP and increased GFR, UFR, UNaV, and the release of CGRP and SP. Capz or CGRP(8-37) combined with Spa fully blocked the effect of Cap on PP, GFR, UFR, UNaV, and the release of CGRP and SP. In conclusion, activation of TRPV1 in the isolated kidney decreases renal PP and increases GFR and water/sodium excretion possibly via simultaneous activation of CGRP and SP receptors upon their enhanced release, suggesting that TRPV1 plays a key role in modulating renal hemodynamics and excretory function. SN - 1043-6618 UR - https://www.unboundmedicine.com/medline/citation/18329285/Increased_GFR_and_renal_excretory_function_by_activation_of_TRPV1_in_the_isolated_perfused_kidney_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1043-6618(08)00021-2 DB - PRIME DP - Unbound Medicine ER -