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Distribution of HLA-B*27 subtypes in Tunisians and their association with ankylosing spondylitis.
Joint Bone Spine. 2008 Mar; 75(2):172-5.JB

Abstract

OBJECTIVE

The human leukocyte antigen HLA-B27 is a class I antigen of the major histocompatibility complex strongly associated with ankylosing spondylitis (AS) and other related spondyloarthropathies (SpAs). The mechanism of this association remains unknown. HLA-B27 is a serologic specificity that represents a family of at least 25 different HLA-B27 alleles (2701-2725). These alleles are closely related by nucleotide sequence homology, but differ in ethnic distribution. The purpose of the present study is to investigate the distribution of HLA-B27 alleles in healthy controls and in patients with ankylosing spondylitis (AS).

METHODS

We selected 160 HLA-B27-positive individuals (39 controls and 121 patients with ankylosing spondylitis). Typing of HLA-B27, and Cw alleles were performed by polymerase chain reaction amplification with sequence specific primers (PCR-SSP), and by serological typing (microlymphocytotoxicity).

RESULTS

Seven B27 subtypes were identified: B*2702, 03, 04, 05, 07, 09 and B*2714. The distribution of these alleles in the population of patients was B*2702 (47.1%) and B*2705 (47.1%). These subtypes were also detected in 16 (41%) and 16 (41%), respectively of the 39 control subjects. HLA-B*2707 was detected in 4 (3.31%) patients and in 3 (7.6%) controls. B*2704, 09 and B*2714 were relatively rare and were detected in one subject each. No significant differences were noticed in the frequencies and distribution of HLA-B27 alleles between patients and controls.

CONCLUSIONS

Our results show a restricted number of HLA B27 subtypes associated with AS. B*2702 and B*2705 were equally common in patients and controls. The most prominent B27/Cw haplotypes in the patient groups and controls were B*2702/Cw02022 and B*2705/Cw02022.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Ben Radhia K
Department of Immunology, EPS Charles Nicolle, Boulevard 9 Avril, 1006 Tunis, Tunisia.
Ayed-Jendoubi S
No affiliation info available
Sfar I
No affiliation info available
Ben Romdhane T
No affiliation info available
Makhlouf M
No affiliation info available
Gorgi Y
No affiliation info available
Ayed K
No affiliation info available

MeSH

AdultAllelesCase-Control StudiesFemaleGenetic Predisposition to DiseaseHLA-B AntigensHLA-B27 AntigenHaplotypesHumansMaleMiddle AgedPolymorphism, GeneticSpondylitis, AnkylosingTunisia

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18329312

Citation

Ben Radhia, Karima, et al. "Distribution of HLA-B*27 Subtypes in Tunisians and Their Association With Ankylosing Spondylitis." Joint Bone Spine, vol. 75, no. 2, 2008, pp. 172-5.
Ben Radhia K, Ayed-Jendoubi S, Sfar I, et al. Distribution of HLA-B*27 subtypes in Tunisians and their association with ankylosing spondylitis. Joint Bone Spine. 2008;75(2):172-5.
Ben Radhia, K., Ayed-Jendoubi, S., Sfar, I., Ben Romdhane, T., Makhlouf, M., Gorgi, Y., & Ayed, K. (2008). Distribution of HLA-B*27 subtypes in Tunisians and their association with ankylosing spondylitis. Joint Bone Spine, 75(2), 172-5. https://doi.org/10.1016/j.jbspin.2007.05.020
Ben Radhia K, et al. Distribution of HLA-B*27 Subtypes in Tunisians and Their Association With Ankylosing Spondylitis. Joint Bone Spine. 2008;75(2):172-5. PubMed PMID: 18329312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of HLA-B*27 subtypes in Tunisians and their association with ankylosing spondylitis. AU - Ben Radhia,Karima, AU - Ayed-Jendoubi,Saloua, AU - Sfar,Imene, AU - Ben Romdhane,Thouraya, AU - Makhlouf,Mouna, AU - Gorgi,Yousr, AU - Ayed,Khaled, Y1 - 2008/01/29/ PY - 2006/12/22/received PY - 2007/05/28/accepted PY - 2008/3/11/pubmed PY - 2008/7/1/medline PY - 2008/3/11/entrez SP - 172 EP - 5 JF - Joint bone spine JO - Joint Bone Spine VL - 75 IS - 2 N2 - OBJECTIVE: The human leukocyte antigen HLA-B27 is a class I antigen of the major histocompatibility complex strongly associated with ankylosing spondylitis (AS) and other related spondyloarthropathies (SpAs). The mechanism of this association remains unknown. HLA-B27 is a serologic specificity that represents a family of at least 25 different HLA-B27 alleles (2701-2725). These alleles are closely related by nucleotide sequence homology, but differ in ethnic distribution. The purpose of the present study is to investigate the distribution of HLA-B27 alleles in healthy controls and in patients with ankylosing spondylitis (AS). METHODS: We selected 160 HLA-B27-positive individuals (39 controls and 121 patients with ankylosing spondylitis). Typing of HLA-B27, and Cw alleles were performed by polymerase chain reaction amplification with sequence specific primers (PCR-SSP), and by serological typing (microlymphocytotoxicity). RESULTS: Seven B27 subtypes were identified: B*2702, 03, 04, 05, 07, 09 and B*2714. The distribution of these alleles in the population of patients was B*2702 (47.1%) and B*2705 (47.1%). These subtypes were also detected in 16 (41%) and 16 (41%), respectively of the 39 control subjects. HLA-B*2707 was detected in 4 (3.31%) patients and in 3 (7.6%) controls. B*2704, 09 and B*2714 were relatively rare and were detected in one subject each. No significant differences were noticed in the frequencies and distribution of HLA-B27 alleles between patients and controls. CONCLUSIONS: Our results show a restricted number of HLA B27 subtypes associated with AS. B*2702 and B*2705 were equally common in patients and controls. The most prominent B27/Cw haplotypes in the patient groups and controls were B*2702/Cw02022 and B*2705/Cw02022. SN - 1778-7254 UR - https://www.unboundmedicine.com/medline/citation/18329312/Distribution_of_HLA_B_27_subtypes_in_Tunisians_and_their_association_with_ankylosing_spondylitis_ DB - PRIME DP - Unbound Medicine ER -