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Effects of omega-3 fatty acids on inflammatory mediators and splenocyte cytokine mRNA expressions in rats with polymicrobial sepsis.
Nutrition. 2008 May; 24(5):484-91.N

Abstract

OBJECTIVES

Many studies have shown that omega-3 fatty acids (FAs) have immunomodulatory effects. However, the influence of omega-3 FAs on septic conditions is not certain. This study examined the effect of fish oil (FO)-enriched diets before and/or omega-3 FA-containing total parenteral nutrition (TPN) after sepsis on the distribution of T-lymphocyte subpopulations and splenocyte cytokine mRNA expressions in rats with polymicrobial sepsis.

METHODS

Rats were assigned to a control group and four experimental groups. The control group and groups 1 and 2 were fed a semipurified diet, and groups 3 and 4 had 20% soybean oil replaced by FO. After feeding the diets for 10 d, sepsis was induced by cecal ligation and puncture (CLP) in the experimental groups, whereas sham operation was performed on the control group. TPN was maintained for 3 d after CLP or sham operation. The control group and groups 1 and 3 were infused with conventional TPN, whereas the TPN solution of groups 2 and 4 was supplemented with FO. All rats were sacrificed 3 d after the operation to examine their immune responses.

RESULTS

Messenger RNA expressions of interleukin-2 and tumor necrosis factor-alpha in splenocytes were higher in groups 3 and 4 than in the control group and group 1. Interleukin-10 mRNA expression in group 3 was higher than in the control group and group 2. Blood CD4 percentage and CD4/CD8 ratio in group 1 were significantly lower, whereas no differences were observed in FO-supplemented groups compared with the control group.

CONCLUSION

FO administration before and/or after CLP maintained blood T-lymphocyte subpopulations and modulated T-helper type 1 and 2 cytokine mRNA expressions in rats with polymicrobial sepsis.

Authors+Show Affiliations

School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18329852

Citation

Tsou, Shung-Sheng, et al. "Effects of Omega-3 Fatty Acids On Inflammatory Mediators and Splenocyte Cytokine mRNA Expressions in Rats With Polymicrobial Sepsis." Nutrition (Burbank, Los Angeles County, Calif.), vol. 24, no. 5, 2008, pp. 484-91.
Tsou SS, Chiu WC, Yeh CL, et al. Effects of omega-3 fatty acids on inflammatory mediators and splenocyte cytokine mRNA expressions in rats with polymicrobial sepsis. Nutrition. 2008;24(5):484-91.
Tsou, S. S., Chiu, W. C., Yeh, C. L., Hou, Y. C., & Yeh, S. L. (2008). Effects of omega-3 fatty acids on inflammatory mediators and splenocyte cytokine mRNA expressions in rats with polymicrobial sepsis. Nutrition (Burbank, Los Angeles County, Calif.), 24(5), 484-91. https://doi.org/10.1016/j.nut.2008.01.012
Tsou SS, et al. Effects of Omega-3 Fatty Acids On Inflammatory Mediators and Splenocyte Cytokine mRNA Expressions in Rats With Polymicrobial Sepsis. Nutrition. 2008;24(5):484-91. PubMed PMID: 18329852.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of omega-3 fatty acids on inflammatory mediators and splenocyte cytokine mRNA expressions in rats with polymicrobial sepsis. AU - Tsou,Shung-Sheng, AU - Chiu,Wan-Chun, AU - Yeh,Chiu-Li, AU - Hou,Yu-Chen, AU - Yeh,Sung-Ling, Y1 - 2008/03/10/ PY - 2007/08/02/received PY - 2008/01/22/revised PY - 2008/01/23/accepted PY - 2008/3/11/pubmed PY - 2008/8/12/medline PY - 2008/3/11/entrez SP - 484 EP - 91 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 24 IS - 5 N2 - OBJECTIVES: Many studies have shown that omega-3 fatty acids (FAs) have immunomodulatory effects. However, the influence of omega-3 FAs on septic conditions is not certain. This study examined the effect of fish oil (FO)-enriched diets before and/or omega-3 FA-containing total parenteral nutrition (TPN) after sepsis on the distribution of T-lymphocyte subpopulations and splenocyte cytokine mRNA expressions in rats with polymicrobial sepsis. METHODS: Rats were assigned to a control group and four experimental groups. The control group and groups 1 and 2 were fed a semipurified diet, and groups 3 and 4 had 20% soybean oil replaced by FO. After feeding the diets for 10 d, sepsis was induced by cecal ligation and puncture (CLP) in the experimental groups, whereas sham operation was performed on the control group. TPN was maintained for 3 d after CLP or sham operation. The control group and groups 1 and 3 were infused with conventional TPN, whereas the TPN solution of groups 2 and 4 was supplemented with FO. All rats were sacrificed 3 d after the operation to examine their immune responses. RESULTS: Messenger RNA expressions of interleukin-2 and tumor necrosis factor-alpha in splenocytes were higher in groups 3 and 4 than in the control group and group 1. Interleukin-10 mRNA expression in group 3 was higher than in the control group and group 2. Blood CD4 percentage and CD4/CD8 ratio in group 1 were significantly lower, whereas no differences were observed in FO-supplemented groups compared with the control group. CONCLUSION: FO administration before and/or after CLP maintained blood T-lymphocyte subpopulations and modulated T-helper type 1 and 2 cytokine mRNA expressions in rats with polymicrobial sepsis. SN - 0899-9007 UR - https://www.unboundmedicine.com/medline/citation/18329852/Effects_of_omega_3_fatty_acids_on_inflammatory_mediators_and_splenocyte_cytokine_mRNA_expressions_in_rats_with_polymicrobial_sepsis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0899-9007(08)00035-X DB - PRIME DP - Unbound Medicine ER -