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[Determination of iridoids and triterpenes in herb of Swertia pseudochinesis by RP-HPLC].
Zhongguo Zhong Yao Za Zhi. 2007 Dec; 32(23):2494-6.ZZ

Abstract

OBJECTIVE

To establish a RP-HPLC method for the determination of swertiamarin, sweroside, gentiopicrin and oleanolic acid in different parts of Swertia pseudochinesis.

METHOD

A Zorbax SB-C18 (4.6 mm x 250 mm, 5 microm) column was used with acetonitrile-water (10:90) and methnol-water(86:14) at detection wavelengths of 238 nm, 246 nm, 274 nm and 207 nm for swertiamarin, sweroside, gentiopicrin and oleanolic acid respectively. The flow rate was 1.0 mL x min(-1) and the column temperature was 25 degrees C.

RESULT

All of the compounds were based--isolated. The linear ranges of swertiamarin, sweroside, gentiopicrin and oleanolic acid were 0.068 9-0.344 4(r = 0.999 2) , 0.001 1-0.014 0 (r2 = 0. 999 8), 0.001 1-0.013 4 (r2 = 0.999 9) and 0.001 1-0.008 8 mg x mL(-1) (r2 = 0. 999 6), respectively.

CONCLUSION

The method is simple and accurate, which can be used for quality control of S. pseudochinesis.

Authors+Show Affiliations

Huang WZ
State Research Center for R&D of TCM Multi-ingredient Drugs, Beijing 100075, China.
Xu CH
No affiliation info available
Zhou DC
No affiliation info available
Tong LH
No affiliation info available
Ye ZG
No affiliation info available

MeSH

Chromatography, High Pressure LiquidFlowersGlucosidesIridoid GlucosidesIridoidsOleanolic AcidPlant LeavesPlant RootsPlant StemsPlants, MedicinalPyronesReproducibility of ResultsSwertiaTriterpenes

Pub Type(s)

Journal Article

Language

chi

PubMed ID

18330241

Citation

Huang, Wen-Zhe, et al. "[Determination of Iridoids and Triterpenes in Herb of Swertia Pseudochinesis By RP-HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 32, no. 23, 2007, pp. 2494-6.
Huang WZ, Xu CH, Zhou DC, et al. [Determination of iridoids and triterpenes in herb of Swertia pseudochinesis by RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2007;32(23):2494-6.
Huang, W. Z., Xu, C. H., Zhou, D. C., Tong, L. H., & Ye, Z. G. (2007). [Determination of iridoids and triterpenes in herb of Swertia pseudochinesis by RP-HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 32(23), 2494-6.
Huang WZ, et al. [Determination of Iridoids and Triterpenes in Herb of Swertia Pseudochinesis By RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2007;32(23):2494-6. PubMed PMID: 18330241.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of iridoids and triterpenes in herb of Swertia pseudochinesis by RP-HPLC]. AU - Huang,Wen-Zhe, AU - Xu,Chun-Hui, AU - Zhou,Da-Cheng, AU - Tong,Li-Hua, AU - Ye,Zu-Guang, PY - 2008/3/12/pubmed PY - 2009/4/3/medline PY - 2008/3/12/entrez SP - 2494 EP - 6 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 32 IS - 23 N2 - OBJECTIVE: To establish a RP-HPLC method for the determination of swertiamarin, sweroside, gentiopicrin and oleanolic acid in different parts of Swertia pseudochinesis. METHOD: A Zorbax SB-C18 (4.6 mm x 250 mm, 5 microm) column was used with acetonitrile-water (10:90) and methnol-water(86:14) at detection wavelengths of 238 nm, 246 nm, 274 nm and 207 nm for swertiamarin, sweroside, gentiopicrin and oleanolic acid respectively. The flow rate was 1.0 mL x min(-1) and the column temperature was 25 degrees C. RESULT: All of the compounds were based--isolated. The linear ranges of swertiamarin, sweroside, gentiopicrin and oleanolic acid were 0.068 9-0.344 4(r = 0.999 2) , 0.001 1-0.014 0 (r2 = 0. 999 8), 0.001 1-0.013 4 (r2 = 0.999 9) and 0.001 1-0.008 8 mg x mL(-1) (r2 = 0. 999 6), respectively. CONCLUSION: The method is simple and accurate, which can be used for quality control of S. pseudochinesis. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/18330241/[Determination_of_iridoids_and_triterpenes_in_herb_of_Swertia_pseudochinesis_by_RP_HPLC]_ DB - PRIME DP - Unbound Medicine ER -