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NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product distribution in Saccharomyces cerevisiae.
Appl Microbiol Biotechnol. 2008 Apr; 78(6):939-45.AM

Abstract

Saccharomyces cerevisiae alcohol dehydrogenases responsible for NADH-, and NADPH-specific reduction of the furaldehydes 5-hydroxymethyl-furfural (HMF) and furfural have previously been identified. In the present study, strains overexpressing the corresponding genes (mut-ADH1 and ADH6), together with a control strain, were compared in defined medium for anaerobic fermentation of glucose in the presence and absence of HMF. All strains showed a similar fermentation pattern in the absence of HMF. In the presence of HMF, the strain overexpressing ADH6 showed the highest HMF reduction rate and the highest specific ethanol productivity, followed by the strain overexpressing mut-ADH1. This correlated with in vitro HMF reduction capacity observed in the ADH6 overexpressing strain. Acetate and glycerol yields per biomass increased considerably in the ADH6 strain. In the other two strains, only the overall acetate yield per biomass was affected. When compared in batch fermentation of spruce hydrolysate, strains overexpressing ADH6 and mut-ADH1 had five times higher HMF uptake rate than the control strain and improved specific ethanol productivity. Overall, our results demonstrate that (1) the cofactor usage in the HMF reduction affects the product distribution, and (2) increased HMF reduction activity results in increased specific ethanol productivity in defined mineral medium and in spruce hydrolysate.

Authors+Show Affiliations

Department of Applied Microbiology, Lund University, Lund, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18330568

Citation

Almeida, João R M., et al. "NADH- Vs NADPH-coupled Reduction of 5-hydroxymethyl Furfural (HMF) and Its Implications On Product Distribution in Saccharomyces Cerevisiae." Applied Microbiology and Biotechnology, vol. 78, no. 6, 2008, pp. 939-45.
Almeida JR, Röder A, Modig T, et al. NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product distribution in Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2008;78(6):939-45.
Almeida, J. R., Röder, A., Modig, T., Laadan, B., Lidén, G., & Gorwa-Grauslund, M. F. (2008). NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product distribution in Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 78(6), 939-45. https://doi.org/10.1007/s00253-008-1364-y
Almeida JR, et al. NADH- Vs NADPH-coupled Reduction of 5-hydroxymethyl Furfural (HMF) and Its Implications On Product Distribution in Saccharomyces Cerevisiae. Appl Microbiol Biotechnol. 2008;78(6):939-45. PubMed PMID: 18330568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product distribution in Saccharomyces cerevisiae. AU - Almeida,João R M, AU - Röder,Anja, AU - Modig,Tobias, AU - Laadan,Boaz, AU - Lidén,Gunnar, AU - Gorwa-Grauslund,Marie-F, Y1 - 2008/03/11/ PY - 2007/11/23/received PY - 2008/01/12/accepted PY - 2008/01/11/revised PY - 2008/3/12/pubmed PY - 2008/7/2/medline PY - 2008/3/12/entrez SP - 939 EP - 45 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 78 IS - 6 N2 - Saccharomyces cerevisiae alcohol dehydrogenases responsible for NADH-, and NADPH-specific reduction of the furaldehydes 5-hydroxymethyl-furfural (HMF) and furfural have previously been identified. In the present study, strains overexpressing the corresponding genes (mut-ADH1 and ADH6), together with a control strain, were compared in defined medium for anaerobic fermentation of glucose in the presence and absence of HMF. All strains showed a similar fermentation pattern in the absence of HMF. In the presence of HMF, the strain overexpressing ADH6 showed the highest HMF reduction rate and the highest specific ethanol productivity, followed by the strain overexpressing mut-ADH1. This correlated with in vitro HMF reduction capacity observed in the ADH6 overexpressing strain. Acetate and glycerol yields per biomass increased considerably in the ADH6 strain. In the other two strains, only the overall acetate yield per biomass was affected. When compared in batch fermentation of spruce hydrolysate, strains overexpressing ADH6 and mut-ADH1 had five times higher HMF uptake rate than the control strain and improved specific ethanol productivity. Overall, our results demonstrate that (1) the cofactor usage in the HMF reduction affects the product distribution, and (2) increased HMF reduction activity results in increased specific ethanol productivity in defined mineral medium and in spruce hydrolysate. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/18330568/NADH__vs_NADPH_coupled_reduction_of_5_hydroxymethyl_furfural__HMF__and_its_implications_on_product_distribution_in_Saccharomyces_cerevisiae_ L2 - https://dx.doi.org/10.1007/s00253-008-1364-y DB - PRIME DP - Unbound Medicine ER -