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Fluorescence in situ hybridization (FISH) analysis of the interactions between honeybee larvae and Paenibacillus larvae, the causative agent of American foulbrood of honeybees (Apis mellifera).
Environ Microbiol. 2008 Jun; 10(6):1612-20.EM

Abstract

American foulbrood (AFB) is a bacterial disease of honeybee larvae caused by the spore-forming bacterium Paenibacillus larvae. Although AFB and its aetiological agent are described now for more than a century, the general and molecular pathogenesis of this notifiable disease is poorly understood. We used fluorescence in situ hybridization (FISH) performed with P. larvae-specific, 16S rRNA-targeted oligonucleotide probes to analyse the early steps in the pathogenesis of American foulbrood. The following chain of events could be demonstrated: (i) the spores germinate in the midgut lumen, (ii) the vegetative bacteria massively proliferate within the midgut before, and (iii) they start to locally breach the epithelium and invade the haemocoel. The paracellular route was shown to be the main mechanism for invasion contrasting earlier hypotheses of phagocytosis of P. larvae. Invasion coincided with the death of the host implicating that the penetration of the midgut epithelium is a critical step determining the time of death.

Authors+Show Affiliations

Institute for Bee Research, Friedrich-Engels-Str. 32, 16540 Hohen Neuendorf, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18331334

Citation

Yue, Dominique, et al. "Fluorescence in Situ Hybridization (FISH) Analysis of the Interactions Between Honeybee Larvae and Paenibacillus Larvae, the Causative Agent of American Foulbrood of Honeybees (Apis Mellifera)." Environmental Microbiology, vol. 10, no. 6, 2008, pp. 1612-20.
Yue D, Nordhoff M, Wieler LH, et al. Fluorescence in situ hybridization (FISH) analysis of the interactions between honeybee larvae and Paenibacillus larvae, the causative agent of American foulbrood of honeybees (Apis mellifera). Environ Microbiol. 2008;10(6):1612-20.
Yue, D., Nordhoff, M., Wieler, L. H., & Genersch, E. (2008). Fluorescence in situ hybridization (FISH) analysis of the interactions between honeybee larvae and Paenibacillus larvae, the causative agent of American foulbrood of honeybees (Apis mellifera). Environmental Microbiology, 10(6), 1612-20. https://doi.org/10.1111/j.1462-2920.2008.01579.x
Yue D, et al. Fluorescence in Situ Hybridization (FISH) Analysis of the Interactions Between Honeybee Larvae and Paenibacillus Larvae, the Causative Agent of American Foulbrood of Honeybees (Apis Mellifera). Environ Microbiol. 2008;10(6):1612-20. PubMed PMID: 18331334.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fluorescence in situ hybridization (FISH) analysis of the interactions between honeybee larvae and Paenibacillus larvae, the causative agent of American foulbrood of honeybees (Apis mellifera). AU - Yue,Dominique, AU - Nordhoff,Marcel, AU - Wieler,Lothar H, AU - Genersch,Elke, Y1 - 2008/03/04/ PY - 2008/3/12/pubmed PY - 2008/6/14/medline PY - 2008/3/12/entrez SP - 1612 EP - 20 JF - Environmental microbiology JO - Environ Microbiol VL - 10 IS - 6 N2 - American foulbrood (AFB) is a bacterial disease of honeybee larvae caused by the spore-forming bacterium Paenibacillus larvae. Although AFB and its aetiological agent are described now for more than a century, the general and molecular pathogenesis of this notifiable disease is poorly understood. We used fluorescence in situ hybridization (FISH) performed with P. larvae-specific, 16S rRNA-targeted oligonucleotide probes to analyse the early steps in the pathogenesis of American foulbrood. The following chain of events could be demonstrated: (i) the spores germinate in the midgut lumen, (ii) the vegetative bacteria massively proliferate within the midgut before, and (iii) they start to locally breach the epithelium and invade the haemocoel. The paracellular route was shown to be the main mechanism for invasion contrasting earlier hypotheses of phagocytosis of P. larvae. Invasion coincided with the death of the host implicating that the penetration of the midgut epithelium is a critical step determining the time of death. SN - 1462-2920 UR - https://www.unboundmedicine.com/medline/citation/18331334/Fluorescence_in_situ_hybridization__FISH__analysis_of_the_interactions_between_honeybee_larvae_and_Paenibacillus_larvae_the_causative_agent_of_American_foulbrood_of_honeybees__Apis_mellifera__ L2 - https://doi.org/10.1111/j.1462-2920.2008.01579.x DB - PRIME DP - Unbound Medicine ER -