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Crucial role of zebrafish prox1 in hypothalamic catecholaminergic neurons development.
BMC Dev Biol. 2008 Mar 10; 8:27.BD

Abstract

BACKGROUND

Prox1, the vertebrate homolog of prospero in Drosophila melanogaster, is a divergent homeogene that regulates cell proliferation, fate determination and differentiation during vertebrate embryonic development.

RESULTS

Here we report that, in zebrafish, prox1 is widely expressed in several districts of the Central Nervous System (CNS). Specifically, we evidenced prox1 expression in a group of neurons, already positive for otp1, located in the hypothalamus at the level of the posterior tuberculum (PT). Prox1 knock-down determines the severe loss of hypothalamic catecholaminergic (CA) neurons, identified by tyrosine hydroxylase (TH) expression, and the synergistic prox1/otp1 overexpression induces the appearance of hypothalamic supernumerary TH-positive neurons and ectopic TH-positive cells on the yolk epitelium.

CONCLUSION

Our findings indicate that prox1 activity is crucial for the proper development of the otp1-positive hypothalamic neuronal precursors to their terminal CA phenotype.

Authors+Show Affiliations

Department of Biology, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy. anna.pistocchi@unimi.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18331627

Citation

Pistocchi, Anna, et al. "Crucial Role of Zebrafish Prox1 in Hypothalamic Catecholaminergic Neurons Development." BMC Developmental Biology, vol. 8, 2008, p. 27.
Pistocchi A, Gaudenzi G, Carra S, et al. Crucial role of zebrafish prox1 in hypothalamic catecholaminergic neurons development. BMC Dev Biol. 2008;8:27.
Pistocchi, A., Gaudenzi, G., Carra, S., Bresciani, E., Del Giacco, L., & Cotelli, F. (2008). Crucial role of zebrafish prox1 in hypothalamic catecholaminergic neurons development. BMC Developmental Biology, 8, 27. https://doi.org/10.1186/1471-213X-8-27
Pistocchi A, et al. Crucial Role of Zebrafish Prox1 in Hypothalamic Catecholaminergic Neurons Development. BMC Dev Biol. 2008 Mar 10;8:27. PubMed PMID: 18331627.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crucial role of zebrafish prox1 in hypothalamic catecholaminergic neurons development. AU - Pistocchi,Anna, AU - Gaudenzi,Germano, AU - Carra,Silvia, AU - Bresciani,Erica, AU - Del Giacco,Luca, AU - Cotelli,Franco, Y1 - 2008/03/10/ PY - 2007/09/20/received PY - 2008/03/10/accepted PY - 2008/3/12/pubmed PY - 2008/5/10/medline PY - 2008/3/12/entrez SP - 27 EP - 27 JF - BMC developmental biology JO - BMC Dev. Biol. VL - 8 N2 - BACKGROUND: Prox1, the vertebrate homolog of prospero in Drosophila melanogaster, is a divergent homeogene that regulates cell proliferation, fate determination and differentiation during vertebrate embryonic development. RESULTS: Here we report that, in zebrafish, prox1 is widely expressed in several districts of the Central Nervous System (CNS). Specifically, we evidenced prox1 expression in a group of neurons, already positive for otp1, located in the hypothalamus at the level of the posterior tuberculum (PT). Prox1 knock-down determines the severe loss of hypothalamic catecholaminergic (CA) neurons, identified by tyrosine hydroxylase (TH) expression, and the synergistic prox1/otp1 overexpression induces the appearance of hypothalamic supernumerary TH-positive neurons and ectopic TH-positive cells on the yolk epitelium. CONCLUSION: Our findings indicate that prox1 activity is crucial for the proper development of the otp1-positive hypothalamic neuronal precursors to their terminal CA phenotype. SN - 1471-213X UR - https://www.unboundmedicine.com/medline/citation/18331627/Crucial_role_of_zebrafish_prox1_in_hypothalamic_catecholaminergic_neurons_development_ L2 - https://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-8-27 DB - PRIME DP - Unbound Medicine ER -