TY - JOUR T1 - Isolation and characterization of new lactam compounds that inhibit lung and colon cancer cells from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) bran. AU - Lee,Ming-Yi, AU - Lin,Huan-You, AU - Cheng,Faiwen, AU - Chiang,Wenchang, AU - Kuo,Yueh-Hsiung, Y1 - 2008/02/12/ PY - 2007/10/27/received PY - 2007/12/19/revised PY - 2008/01/19/accepted PY - 2008/3/12/pubmed PY - 2008/7/11/medline PY - 2008/3/12/entrez SP - 1933 EP - 9 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 46 IS - 6 N2 - Five active compounds that inhibit cancer cells were isolated from adlay bran (Coix lachryma-jobi L. var. ma-yuen Stapf), and their structures and activities in vitro were characterized. The ethyl acetate-soluble fraction of methanol extracts of adlay bran (ABM-EtOAc) exhibited a stronger anti-proliferative effect on human lung cancer cell A549, human colorectal carcinoma cell HT-29, and COLO 205 than other fractions by MTT (3-4,5-dimethylthiazol-2-yl-2,5-diphenyl-tetrazolium bromide) assay. Assay-guided isolation gave five lactams including three that were previously undocumented; coixspirolactam A (1), coixspirolactam B (2), and coixspirolactam C (3); one isolated from the natural plant for the first time, coixlactam (4); and one known compound, methyl dioxindole-3-acetate (5). Pure active compounds were identified by spectral analysis including IR, 1H and 13C NMR, UV-vis, MS and 2D NMR techniques. All the compounds were tested for their anti-proliferative effect on A549, HT-29 and COLO 205 cells. These compounds showed anti-cancer activities with IC50 values between 28.6 and 72.6microg/mL. SN - 0278-6915 UR - https://www.unboundmedicine.com/medline/citation/18331775/Isolation_and_characterization_of_new_lactam_compounds_that_inhibit_lung_and_colon_cancer_cells_from_adlay__Coix_lachryma_jobi_L__var__ma_yuen_Stapf__bran_ DB - PRIME DP - Unbound Medicine ER -