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Effects of poly-N-acetyl glucosamine (pGlcNAc) patch on wound healing in db/db mouse.

Abstract

BACKGROUND

Poly-N-acetyl glucosamine (pGlcNAc) nanofiber-based materials, produced by a marine microalga, have been characterized as effective hemostatic agents. In this study, we hypothesized that a pGlcNAc fiber patch may enhance wound healing in the db/db mouse.

METHODS

pGlcNAc patches were applied on 1-cm, full-thickness, skin wounds in the db/db mouse model. Wounds (n = 15 per group) were dressed with a pGlcNAc nanofiber patch for 1 hour, 24 hours, or left untreated. After the application time, patches were removed and wounds were allowed to heal spontaneously. The rate of wound closure was evaluated by digital analysis of unclosed wound area as a function of time. At day 10, wounds (n = 7 per group) were harvested and quantified with immunohistochemical markers of proliferation (Ki-67) and vascularization (platelet endothelial cell adhesion molecule).

RESULTS

Wounds dressed with pGlcNAc patches for 1 hour closed faster than control wounds, reaching 90% closure in 16.6 days, 9 days faster than untreated wounds. Granulation tissue showed higher levels of proliferation and vascularization after 1-hour treatment than the 24-hour and left-untreated groups. Foreign body reaction to the material was not noted in applications up to 24 hours.

DISCUSSION

In addition to its hemostatic properties, the pGlcNAc material also appears to accelerate wound closure in healing-impaired genetically diabetic mice. This material, with its combination of hemostatic and wound healing properties, has the potential to be effective agent for the treatment of complicated wounds.

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  • Authors+Show Affiliations

    ,

    Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

    , , , , , , , , , ,

    Source

    The Journal of trauma 64:3 2008 Mar pg 803-8

    MeSH

    Acetylglucosamine
    Analysis of Variance
    Animals
    Bandages
    Diabetes Mellitus, Experimental
    Disease Models, Animal
    Male
    Mice
    Mice, Inbred C57BL
    Skin
    Wound Healing

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    18332827

    Citation

    Pietramaggiori, Giorgio, et al. "Effects of poly-N-acetyl Glucosamine (pGlcNAc) Patch On Wound Healing in Db/db Mouse." The Journal of Trauma, vol. 64, no. 3, 2008, pp. 803-8.
    Pietramaggiori G, Yang HJ, Scherer SS, et al. Effects of poly-N-acetyl glucosamine (pGlcNAc) patch on wound healing in db/db mouse. J Trauma. 2008;64(3):803-8.
    Pietramaggiori, G., Yang, H. J., Scherer, S. S., Kaipainen, A., Chan, R. K., Alperovich, M., ... Orgill, D. P. (2008). Effects of poly-N-acetyl glucosamine (pGlcNAc) patch on wound healing in db/db mouse. The Journal of Trauma, 64(3), pp. 803-8. doi:10.1097/01.ta.0000244382.13937.a8.
    Pietramaggiori G, et al. Effects of poly-N-acetyl Glucosamine (pGlcNAc) Patch On Wound Healing in Db/db Mouse. J Trauma. 2008;64(3):803-8. PubMed PMID: 18332827.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effects of poly-N-acetyl glucosamine (pGlcNAc) patch on wound healing in db/db mouse. AU - Pietramaggiori,Giorgio, AU - Yang,Ho-Jik, AU - Scherer,Saja S, AU - Kaipainen,Arja, AU - Chan,Rodney K, AU - Alperovich,Michael, AU - Newalder,Jennifer, AU - Demcheva,Marina, AU - Vournakis,John N, AU - Valeri,C Robert, AU - Hechtman,Herbert B, AU - Orgill,Dennis P, PY - 2008/3/12/pubmed PY - 2008/4/11/medline PY - 2008/3/12/entrez SP - 803 EP - 8 JF - The Journal of trauma JO - J Trauma VL - 64 IS - 3 N2 - BACKGROUND: Poly-N-acetyl glucosamine (pGlcNAc) nanofiber-based materials, produced by a marine microalga, have been characterized as effective hemostatic agents. In this study, we hypothesized that a pGlcNAc fiber patch may enhance wound healing in the db/db mouse. METHODS: pGlcNAc patches were applied on 1-cm, full-thickness, skin wounds in the db/db mouse model. Wounds (n = 15 per group) were dressed with a pGlcNAc nanofiber patch for 1 hour, 24 hours, or left untreated. After the application time, patches were removed and wounds were allowed to heal spontaneously. The rate of wound closure was evaluated by digital analysis of unclosed wound area as a function of time. At day 10, wounds (n = 7 per group) were harvested and quantified with immunohistochemical markers of proliferation (Ki-67) and vascularization (platelet endothelial cell adhesion molecule). RESULTS: Wounds dressed with pGlcNAc patches for 1 hour closed faster than control wounds, reaching 90% closure in 16.6 days, 9 days faster than untreated wounds. Granulation tissue showed higher levels of proliferation and vascularization after 1-hour treatment than the 24-hour and left-untreated groups. Foreign body reaction to the material was not noted in applications up to 24 hours. DISCUSSION: In addition to its hemostatic properties, the pGlcNAc material also appears to accelerate wound closure in healing-impaired genetically diabetic mice. This material, with its combination of hemostatic and wound healing properties, has the potential to be effective agent for the treatment of complicated wounds. SN - 1529-8809 UR - https://www.unboundmedicine.com/medline/citation/18332827/Effects_of_poly_N_acetyl_glucosamine__pGlcNAc__patch_on_wound_healing_in_db/db_mouse_ L2 - http://Insights.ovid.com/pubmed?pmid=18332827 DB - PRIME DP - Unbound Medicine ER -