Tags

Type your tag names separated by a space and hit enter

Endothelium removal augments endothelium-independent vasodilatation in rat mesenteric vascular bed.
Br J Pharmacol. 2008 May; 154(1):32-40.BJ

Abstract

BACKGROUND AND PURPOSE

The vascular endothelium regulates vascular tone by releasing various endothelium-derived vasoactive substances to counteract excess vascular response. We investigated whether the vascular endothelium regulates vasodilatation via released endothelium-derived contracting factors (EDCFs), by examining the effect of endothelium removal on responses to periarterial nerve stimulation (PNS) and various vasodilator agents.

EXPERIMENTAL APPROACH

The rat mesenteric vascular bed was perfused with Krebs solution. Vasodilator responses to PNS and 5 min perfusion of vasodilator agents in preparations with endothelium were compared with those in the same preparations without endothelium. The endothelium was removed by 30 s perfusion with sodium deoxycholate.

KEY RESULTS

Endothelium removal significantly augmented vasodilator responses to PNS and calcitonin gene-related peptide (CGRP), isoprenaline (beta-adrenoceptor agonist), SNP and 8-bromo-cGMP (8-Br-cGMP; cGMP analogue) but not BAY41-2272 (soluble guanylate cyclase activator). The augmentation of SNP-induced vasodilatation after denudation was much greater than that of CGRP- or isoprenaline-induced vasodilatation. In the preparations with an intact endothelium, L-NAME (nitric oxide synthase inhibitor) significantly augmented vasodilator responses to PNS and CGRP, isoprenaline, SNP and 8-Br-cGMP, but not BAY41-2272. Indomethacin (cyclooxygenase inhibitor) and seratrodast (thromboxane A(2) receptor antagonist), but not phosphoramidon (endothelin-1-converting enzyme inhibitor) or BQ-123 (selective endothelin type A receptor antagonists), significantly augmented vasodilator responses to PNS and CGRP, isoprenaline, SNP and BAY41-2272.

CONCLUSION AND IMPLICATION

These results suggest that the endothelium in rat mesenteric arteries regulates and maintains vascular tone via counteracting not only vasoconstriction through releasing endothelium-derived relaxing factors, but also vasodilatation, in part by releasing an EDCF, thromboxane A(2).

Authors+Show Affiliations

Department of Clinical Pharmaceutical Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18332859

Citation

Iwatani, Y, et al. "Endothelium Removal Augments Endothelium-independent Vasodilatation in Rat Mesenteric Vascular Bed." British Journal of Pharmacology, vol. 154, no. 1, 2008, pp. 32-40.
Iwatani Y, Kosugi K, Isobe-Oku S, et al. Endothelium removal augments endothelium-independent vasodilatation in rat mesenteric vascular bed. Br J Pharmacol. 2008;154(1):32-40.
Iwatani, Y., Kosugi, K., Isobe-Oku, S., Atagi, S., Kitamura, Y., & Kawasaki, H. (2008). Endothelium removal augments endothelium-independent vasodilatation in rat mesenteric vascular bed. British Journal of Pharmacology, 154(1), 32-40. https://doi.org/10.1038/bjp.2008.72
Iwatani Y, et al. Endothelium Removal Augments Endothelium-independent Vasodilatation in Rat Mesenteric Vascular Bed. Br J Pharmacol. 2008;154(1):32-40. PubMed PMID: 18332859.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endothelium removal augments endothelium-independent vasodilatation in rat mesenteric vascular bed. AU - Iwatani,Y, AU - Kosugi,K, AU - Isobe-Oku,S, AU - Atagi,S, AU - Kitamura,Y, AU - Kawasaki,H, Y1 - 2008/03/10/ PY - 2008/3/12/pubmed PY - 2008/7/18/medline PY - 2008/3/12/entrez SP - 32 EP - 40 JF - British journal of pharmacology JO - Br J Pharmacol VL - 154 IS - 1 N2 - BACKGROUND AND PURPOSE: The vascular endothelium regulates vascular tone by releasing various endothelium-derived vasoactive substances to counteract excess vascular response. We investigated whether the vascular endothelium regulates vasodilatation via released endothelium-derived contracting factors (EDCFs), by examining the effect of endothelium removal on responses to periarterial nerve stimulation (PNS) and various vasodilator agents. EXPERIMENTAL APPROACH: The rat mesenteric vascular bed was perfused with Krebs solution. Vasodilator responses to PNS and 5 min perfusion of vasodilator agents in preparations with endothelium were compared with those in the same preparations without endothelium. The endothelium was removed by 30 s perfusion with sodium deoxycholate. KEY RESULTS: Endothelium removal significantly augmented vasodilator responses to PNS and calcitonin gene-related peptide (CGRP), isoprenaline (beta-adrenoceptor agonist), SNP and 8-bromo-cGMP (8-Br-cGMP; cGMP analogue) but not BAY41-2272 (soluble guanylate cyclase activator). The augmentation of SNP-induced vasodilatation after denudation was much greater than that of CGRP- or isoprenaline-induced vasodilatation. In the preparations with an intact endothelium, L-NAME (nitric oxide synthase inhibitor) significantly augmented vasodilator responses to PNS and CGRP, isoprenaline, SNP and 8-Br-cGMP, but not BAY41-2272. Indomethacin (cyclooxygenase inhibitor) and seratrodast (thromboxane A(2) receptor antagonist), but not phosphoramidon (endothelin-1-converting enzyme inhibitor) or BQ-123 (selective endothelin type A receptor antagonists), significantly augmented vasodilator responses to PNS and CGRP, isoprenaline, SNP and BAY41-2272. CONCLUSION AND IMPLICATION: These results suggest that the endothelium in rat mesenteric arteries regulates and maintains vascular tone via counteracting not only vasoconstriction through releasing endothelium-derived relaxing factors, but also vasodilatation, in part by releasing an EDCF, thromboxane A(2). SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/18332859/Endothelium_removal_augments_endothelium_independent_vasodilatation_in_rat_mesenteric_vascular_bed_ L2 - https://doi.org/10.1038/bjp.2008.72 DB - PRIME DP - Unbound Medicine ER -