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Preventive effects of genistein on leukocyte adhesion in femur venules and on bone-loss induced in ovariectomized female rats.
Clin Hemorheol Microcirc. 2008; 38(4):235-43.CH

Abstract

This study was aimed to examine effects of genistein on leukocyte adhesion in femur microcirculation in relation to bone-loss induced in ovariectomized female rats. Sixty-four female Wistar rats were divided into 4 groups: sham (daily treated with vehicle; DMSO, sc; 100 microl/day), ovariectomized rat treated with vehicle (OVX(veh)), 17beta-estradiol treated-ovariectomized rat (OVX(E2), 5 microg/kg/day, s.c.) and genistein treated-ovariectomized rat (0.25 mg/kg/day, s.c.; OVX(gen)). One and three weeks after the ovariectomy, blood flow perfusion (BF) in femur tissue was measured using laser Doppler flowmetry. Leukocyte adhesion in femur venules (15-30 microm in diameter) of each group was evaluated by intravital fluorescence videomicroscopy. The bone mineral content (BMC) was measured and expressed in terms of ratio of ash-to-dry matter weight. Serum osteocalcin and alkaline phosphatase levels were determined using chemiluminescence immunoassay. In both one and three week-OVX(veh), leukocyte adhesion increased significantly, compared to their age-matched sham groups, but it decreased significantly in OVX(gen), compared to OVX(veh) (p<0.05). In three week-OVX(gen), both BF and BMC increased significantly, but osteocalcin and alkaline phosphatase levels decreased, compared to those of three week-OVX(veh). In conclusion, genistein supplementation could effectively prevent bone-loss and microvascular endothelial dysfunction induced by estrogen deficiency.

Authors+Show Affiliations

Department of Sport Science, Faculty of Physical Education, Srinakharinwirot University, Bangkok 10110, Thailand.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18334778

Citation

Kasiyaphat, Atchareeya, et al. "Preventive Effects of Genistein On Leukocyte Adhesion in Femur Venules and On Bone-loss Induced in Ovariectomized Female Rats." Clinical Hemorheology and Microcirculation, vol. 38, no. 4, 2008, pp. 235-43.
Kasiyaphat A, Siriviriyakul P, Patumraj S. Preventive effects of genistein on leukocyte adhesion in femur venules and on bone-loss induced in ovariectomized female rats. Clin Hemorheol Microcirc. 2008;38(4):235-43.
Kasiyaphat, A., Siriviriyakul, P., & Patumraj, S. (2008). Preventive effects of genistein on leukocyte adhesion in femur venules and on bone-loss induced in ovariectomized female rats. Clinical Hemorheology and Microcirculation, 38(4), 235-43.
Kasiyaphat A, Siriviriyakul P, Patumraj S. Preventive Effects of Genistein On Leukocyte Adhesion in Femur Venules and On Bone-loss Induced in Ovariectomized Female Rats. Clin Hemorheol Microcirc. 2008;38(4):235-43. PubMed PMID: 18334778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preventive effects of genistein on leukocyte adhesion in femur venules and on bone-loss induced in ovariectomized female rats. AU - Kasiyaphat,Atchareeya, AU - Siriviriyakul,Prasong, AU - Patumraj,Suthiluk, PY - 2008/3/13/pubmed PY - 2008/5/14/medline PY - 2008/3/13/entrez SP - 235 EP - 43 JF - Clinical hemorheology and microcirculation JO - Clin Hemorheol Microcirc VL - 38 IS - 4 N2 - This study was aimed to examine effects of genistein on leukocyte adhesion in femur microcirculation in relation to bone-loss induced in ovariectomized female rats. Sixty-four female Wistar rats were divided into 4 groups: sham (daily treated with vehicle; DMSO, sc; 100 microl/day), ovariectomized rat treated with vehicle (OVX(veh)), 17beta-estradiol treated-ovariectomized rat (OVX(E2), 5 microg/kg/day, s.c.) and genistein treated-ovariectomized rat (0.25 mg/kg/day, s.c.; OVX(gen)). One and three weeks after the ovariectomy, blood flow perfusion (BF) in femur tissue was measured using laser Doppler flowmetry. Leukocyte adhesion in femur venules (15-30 microm in diameter) of each group was evaluated by intravital fluorescence videomicroscopy. The bone mineral content (BMC) was measured and expressed in terms of ratio of ash-to-dry matter weight. Serum osteocalcin and alkaline phosphatase levels were determined using chemiluminescence immunoassay. In both one and three week-OVX(veh), leukocyte adhesion increased significantly, compared to their age-matched sham groups, but it decreased significantly in OVX(gen), compared to OVX(veh) (p<0.05). In three week-OVX(gen), both BF and BMC increased significantly, but osteocalcin and alkaline phosphatase levels decreased, compared to those of three week-OVX(veh). In conclusion, genistein supplementation could effectively prevent bone-loss and microvascular endothelial dysfunction induced by estrogen deficiency. SN - 1386-0291 UR - https://www.unboundmedicine.com/medline/citation/18334778/Preventive_effects_of_genistein_on_leukocyte_adhesion_in_femur_venules_and_on_bone_loss_induced_in_ovariectomized_female_rats_ L2 - https://content.iospress.com/openurl?genre=article&amp;issn=1386-0291&amp;volume=38&amp;issue=4&amp;spage=235 DB - PRIME DP - Unbound Medicine ER -