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Protective effect of Castanea sativa and Quercus robur leaf extracts against oxygen and nitrogen reactive species.
J Photochem Photobiol B. 2008 May 29; 91(2-3):87-95.JP

Abstract

Topical natural antioxidants are a useful strategy for the prevention of photoaging and oxidative stress mediated skin diseases. In view of this underlying principle, the screening of natural plant extracts with scavenging activity for pro-oxidant reactive species is a primary requirement for the development of new topical antioxidant formulations. In the present study, an ethanol:water (7:3) extract from Castanea sativa leaves and a ethanol:water (2:3) extract from Quercus robur leaves were evaluated for their putative in vitro scavenging effects on reactive oxygen species (ROS) namely superoxide radical (O(2)(-)), hydroxyl radical (HO()), peroxyl radical (ROO()), hydrogen peroxide (H(2)O(2)) and singlet oxygen ((1)O(2)) as well as on reactive nitrogen species (RNS) namely nitric oxide (()NO) and peroxynitrite (ONOO(-)). The extracts presented a high potency to scavenge the tested reactive species, all the IC(50)s being found at the microg/mL level. IC(50)s (mean+/-SE) for the ROS O(2)(-),HO(),H(2)O(2) and (1)O(2) were 13.6+/-1.8; 216+/-4; 410+/-8; 12.3+/-0.7 microug/mL, respectively, for C. sativa, and 11.0+/-0.5; 285+/-22; 251+/-32; 7.90+/-0.56 microg/mL, respectively, for Q. robur. The ORAC values obtained for ROO() were 1.24+/-0.13 for C. sativa and 1.09+/-0.06 for Q. robur. The IC(50)s (mean+/-SE) for ()NO and ONOO(-) were 3.10+/-0.14 and 1.49+/-0.10 microg/mL, respectively, for C. sativa and 3.13+/-0.11 and 0.95+/-0.02 microg/mL, respectively, for Q. robur. The content of total phenolics for C. sativa and Q. robur were 284+/-9 and 346+/-4 mg of gallic acid equivalents (GAE)/g of lyophilized extract respectively. The observed effects might be of relevance considering the putative interest of these extracts as topical antioxidants.

Authors+Show Affiliations

Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia da, Universidade do Porto, Rua Aníbal Cunha 164, 4099-030 Porto, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18337113

Citation

Almeida, Isabel F., et al. "Protective Effect of Castanea Sativa and Quercus Robur Leaf Extracts Against Oxygen and Nitrogen Reactive Species." Journal of Photochemistry and Photobiology. B, Biology, vol. 91, no. 2-3, 2008, pp. 87-95.
Almeida IF, Fernandes E, Lima JL, et al. Protective effect of Castanea sativa and Quercus robur leaf extracts against oxygen and nitrogen reactive species. J Photochem Photobiol B. 2008;91(2-3):87-95.
Almeida, I. F., Fernandes, E., Lima, J. L., Costa, P. C., & Bahia, M. F. (2008). Protective effect of Castanea sativa and Quercus robur leaf extracts against oxygen and nitrogen reactive species. Journal of Photochemistry and Photobiology. B, Biology, 91(2-3), 87-95. https://doi.org/10.1016/j.jphotobiol.2008.02.001
Almeida IF, et al. Protective Effect of Castanea Sativa and Quercus Robur Leaf Extracts Against Oxygen and Nitrogen Reactive Species. J Photochem Photobiol B. 2008 May 29;91(2-3):87-95. PubMed PMID: 18337113.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of Castanea sativa and Quercus robur leaf extracts against oxygen and nitrogen reactive species. AU - Almeida,Isabel F, AU - Fernandes,Eduarda, AU - Lima,José L F C, AU - Costa,P C, AU - Bahia,M F, Y1 - 2008/02/08/ PY - 2007/11/12/received PY - 2008/02/01/revised PY - 2008/02/01/accepted PY - 2008/3/14/pubmed PY - 2008/10/2/medline PY - 2008/3/14/entrez SP - 87 EP - 95 JF - Journal of photochemistry and photobiology. B, Biology JO - J Photochem Photobiol B VL - 91 IS - 2-3 N2 - Topical natural antioxidants are a useful strategy for the prevention of photoaging and oxidative stress mediated skin diseases. In view of this underlying principle, the screening of natural plant extracts with scavenging activity for pro-oxidant reactive species is a primary requirement for the development of new topical antioxidant formulations. In the present study, an ethanol:water (7:3) extract from Castanea sativa leaves and a ethanol:water (2:3) extract from Quercus robur leaves were evaluated for their putative in vitro scavenging effects on reactive oxygen species (ROS) namely superoxide radical (O(2)(-)), hydroxyl radical (HO()), peroxyl radical (ROO()), hydrogen peroxide (H(2)O(2)) and singlet oxygen ((1)O(2)) as well as on reactive nitrogen species (RNS) namely nitric oxide (()NO) and peroxynitrite (ONOO(-)). The extracts presented a high potency to scavenge the tested reactive species, all the IC(50)s being found at the microg/mL level. IC(50)s (mean+/-SE) for the ROS O(2)(-),HO(),H(2)O(2) and (1)O(2) were 13.6+/-1.8; 216+/-4; 410+/-8; 12.3+/-0.7 microug/mL, respectively, for C. sativa, and 11.0+/-0.5; 285+/-22; 251+/-32; 7.90+/-0.56 microg/mL, respectively, for Q. robur. The ORAC values obtained for ROO() were 1.24+/-0.13 for C. sativa and 1.09+/-0.06 for Q. robur. The IC(50)s (mean+/-SE) for ()NO and ONOO(-) were 3.10+/-0.14 and 1.49+/-0.10 microg/mL, respectively, for C. sativa and 3.13+/-0.11 and 0.95+/-0.02 microg/mL, respectively, for Q. robur. The content of total phenolics for C. sativa and Q. robur were 284+/-9 and 346+/-4 mg of gallic acid equivalents (GAE)/g of lyophilized extract respectively. The observed effects might be of relevance considering the putative interest of these extracts as topical antioxidants. SN - 1011-1344 UR - https://www.unboundmedicine.com/medline/citation/18337113/Protective_effect_of_Castanea_sativa_and_Quercus_robur_leaf_extracts_against_oxygen_and_nitrogen_reactive_species_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1011-1344(08)00038-9 DB - PRIME DP - Unbound Medicine ER -