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Cloning and mRNA expression of antioxidant enzymes in the Pacific oyster, Crassostrea gigas in response to cadmium exposure.
Comp Biochem Physiol C Toxicol Pharmacol. 2008 May; 147(4):460-9.CB

Abstract

Cadmium (Cd) is one of the most toxic heavy metal pollutants in the aquatic environment and can induce the formation of reactive oxygen species (ROS) that cause oxidative stress. In present study, we cloned catalase (CAT) and glutathione peroxidase (GPX) cDNA, and investigated its time- and dose-related effects of three Cd concentrations (0.01, 0.05 or 0.1 ppm) on mRNA levels of antioxidant enzymes (superoxide dismutase (SOD), CAT, GPX) in the gill and changes enzyme levels in the hemolymph of the Pacific oyster, Crassostrea gigas. The cDNA indentified encoded proteins of 516 and 244 amino acids corresponding to CAT and GPX, respectively. BLAST analysis from other species indicated that the residues essential to the enzymatic function of CAT and GPX proteins of C. gigas are highly conserved. Cd treatment significantly increased antioxidant enzyme mRNA expression in the gill in a time- and dose-dependent manner. The mRNA expression at 0.1 ppm Cd concentration increased up to 3 days (CAT, GPX) or 7 days (SOD) and then decreased by 7 days (CAT, GPX) or 11 days (SOD). Aspartate aminotransferase, alanine amintransferase and hydrogen peroxide (H(2)O(2)) concentrations levels increased significantly with exposure to 0.05 or 0.1 ppm Cd for 7 days. These results suggest that antioxidant enzymes play important roles in the physiological changes related to metabolism and cell protection that occur in Pacific oysters exposed to Cd.

Authors+Show Affiliations

Division of Marine Environment & Bioscience, Korea Maritime University, Busan 606-791, Republic of Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18337187

Citation

Jo, Pil Gue, et al. "Cloning and mRNA Expression of Antioxidant Enzymes in the Pacific Oyster, Crassostrea Gigas in Response to Cadmium Exposure." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 147, no. 4, 2008, pp. 460-9.
Jo PG, Choi YK, Choi CY. Cloning and mRNA expression of antioxidant enzymes in the Pacific oyster, Crassostrea gigas in response to cadmium exposure. Comp Biochem Physiol C Toxicol Pharmacol. 2008;147(4):460-9.
Jo, P. G., Choi, Y. K., & Choi, C. Y. (2008). Cloning and mRNA expression of antioxidant enzymes in the Pacific oyster, Crassostrea gigas in response to cadmium exposure. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 147(4), 460-9. https://doi.org/10.1016/j.cbpc.2008.02.001
Jo PG, Choi YK, Choi CY. Cloning and mRNA Expression of Antioxidant Enzymes in the Pacific Oyster, Crassostrea Gigas in Response to Cadmium Exposure. Comp Biochem Physiol C Toxicol Pharmacol. 2008;147(4):460-9. PubMed PMID: 18337187.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and mRNA expression of antioxidant enzymes in the Pacific oyster, Crassostrea gigas in response to cadmium exposure. AU - Jo,Pil Gue, AU - Choi,Yong Ki, AU - Choi,Cheol Young, Y1 - 2008/02/11/ PY - 2007/12/27/received PY - 2008/02/02/revised PY - 2008/02/02/accepted PY - 2008/3/14/pubmed PY - 2008/6/20/medline PY - 2008/3/14/entrez SP - 460 EP - 9 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 147 IS - 4 N2 - Cadmium (Cd) is one of the most toxic heavy metal pollutants in the aquatic environment and can induce the formation of reactive oxygen species (ROS) that cause oxidative stress. In present study, we cloned catalase (CAT) and glutathione peroxidase (GPX) cDNA, and investigated its time- and dose-related effects of three Cd concentrations (0.01, 0.05 or 0.1 ppm) on mRNA levels of antioxidant enzymes (superoxide dismutase (SOD), CAT, GPX) in the gill and changes enzyme levels in the hemolymph of the Pacific oyster, Crassostrea gigas. The cDNA indentified encoded proteins of 516 and 244 amino acids corresponding to CAT and GPX, respectively. BLAST analysis from other species indicated that the residues essential to the enzymatic function of CAT and GPX proteins of C. gigas are highly conserved. Cd treatment significantly increased antioxidant enzyme mRNA expression in the gill in a time- and dose-dependent manner. The mRNA expression at 0.1 ppm Cd concentration increased up to 3 days (CAT, GPX) or 7 days (SOD) and then decreased by 7 days (CAT, GPX) or 11 days (SOD). Aspartate aminotransferase, alanine amintransferase and hydrogen peroxide (H(2)O(2)) concentrations levels increased significantly with exposure to 0.05 or 0.1 ppm Cd for 7 days. These results suggest that antioxidant enzymes play important roles in the physiological changes related to metabolism and cell protection that occur in Pacific oysters exposed to Cd. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/18337187/Cloning_and_mRNA_expression_of_antioxidant_enzymes_in_the_Pacific_oyster_Crassostrea_gigas_in_response_to_cadmium_exposure_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(08)00018-5 DB - PRIME DP - Unbound Medicine ER -