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5' Untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation.
J Microbiol. 2008 Feb; 46(1):56-61.JM

Abstract

The sigmas subunit of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genes in Gram-negative bacteria. The Pseudomonas putida rpoS gene is transcribed as a monocistronic rpoS mRNA with a 368 nucleotide-long 5' untranslated region (5' UTR). In this study, we investigate the posttranscriptional control of RpoS synthesis using rpoS-lacZ transcriptional and translational fusions consisting of the native promoter and deletions of 5' UTR or insertion into UTR. The differing activity of constructed translational fusions strongly indicated that the 5' UTR is involved in the translational regulation of RpoS expression in the stationary phase. The results obtained herein demonstrated that the structure of UTR performs an important function in the translational regulation of the rpoS gene.

Authors+Show Affiliations

Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444/a, PO Box 23, 11010 Belgrade, Serbia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18337694

Citation

Jovcic, Branko, et al. "5' Untranslated Region of the Pseudomonas Putida WCS358 Stationary Phase Sigma Factor rpoS mRNA Is Involved in RpoS Translational Regulation." Journal of Microbiology (Seoul, Korea), vol. 46, no. 1, 2008, pp. 56-61.
Jovcic B, Bertani I, Venturi V, et al. 5' Untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation. J Microbiol. 2008;46(1):56-61.
Jovcic, B., Bertani, I., Venturi, V., Topisirovic, L., & Kojic, M. (2008). 5' Untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation. Journal of Microbiology (Seoul, Korea), 46(1), 56-61. https://doi.org/10.1007/s12275-007-0127-2
Jovcic B, et al. 5' Untranslated Region of the Pseudomonas Putida WCS358 Stationary Phase Sigma Factor rpoS mRNA Is Involved in RpoS Translational Regulation. J Microbiol. 2008;46(1):56-61. PubMed PMID: 18337694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 5' Untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation. AU - Jovcic,Branko, AU - Bertani,Iris, AU - Venturi,Vittorio, AU - Topisirovic,Ljubisa, AU - Kojic,Milan, PY - 2008/3/14/pubmed PY - 2008/5/14/medline PY - 2008/3/14/entrez SP - 56 EP - 61 JF - Journal of microbiology (Seoul, Korea) JO - J Microbiol VL - 46 IS - 1 N2 - The sigmas subunit of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genes in Gram-negative bacteria. The Pseudomonas putida rpoS gene is transcribed as a monocistronic rpoS mRNA with a 368 nucleotide-long 5' untranslated region (5' UTR). In this study, we investigate the posttranscriptional control of RpoS synthesis using rpoS-lacZ transcriptional and translational fusions consisting of the native promoter and deletions of 5' UTR or insertion into UTR. The differing activity of constructed translational fusions strongly indicated that the 5' UTR is involved in the translational regulation of RpoS expression in the stationary phase. The results obtained herein demonstrated that the structure of UTR performs an important function in the translational regulation of the rpoS gene. SN - 1225-8873 UR - https://www.unboundmedicine.com/medline/citation/18337694/5'_Untranslated_region_of_the_Pseudomonas_putida_WCS358_stationary_phase_sigma_factor_rpoS_mRNA_is_involved_in_RpoS_translational_regulation_ L2 - https://dx.doi.org/10.1007/s12275-007-0127-2 DB - PRIME DP - Unbound Medicine ER -