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AB-QTL analysis in winter wheat: II. Genetic analysis of seedling and field resistance against leaf rust in a wheat advanced backcross population.
Theor Appl Genet. 2008 May; 116(8):1095-104.TA

Abstract

The present study aimed to localize exotic quantitative trait locus (QTL) alleles for the improvement of leaf rust (P. triticina) resistance in an advanced backcross (AB) population, B22, which is derived from a cross between the winter wheat cultivar Batis (Triticum aestivum) and the synthetic wheat accession Syn022L. The latter was developed from hybridization of T. turgidum ssp. dicoccoides and T. tauschii. Altogether, 250 BC2F3 lines of B22 were assessed for seedling resistance against the leaf rust isolate 77WxR under controlled conditions. In addition, field resistance against leaf rust was evaluated by assessing symptom severity under natural infestation across multiple environments. Simultaneously, population B22 was genotyped with a total of 97 SSR markers, distributed over the wheat A, B and D genomes. The phenotype and genotype data were subjected to QTL analysis by applying a 3-factorial mixed model analysis of variance including the marker genotype as a fixed effect and the environments, the lines and the marker by environment interactions as random effects. The QTL analysis revealed six putative QTLs for seedling resistance and seven for field resistance. For seedling resistance, the effects of exotic QTL alleles improved resistance at all detected loci. The maximum decrease of disease symptoms (-46.3%) was associated with marker locus Xbarc149 on chromosome 1D. For field resistance, two loci had stable main effects across environments and five loci exhibited marker by environment interaction effects. The strongest effects were detected at marker locus Xbarc149 on chromosome 1D, at which the exotic allele decreased seedling symptoms by 46.3% and field symptoms by 43.6%, respectively. Some of the detected QTLs co-localized with known resistance genes, while others appear to be as novel resistance loci. Our findings indicate, that the exotic wheat accession Syn022L may be useful for the improvement of leaf rust resistance in cultivated wheat.

Authors+Show Affiliations

Institute of Crop Science and Resource Conservation, Crop Genetics and Biotechnology Unit, University of Bonn, Katzenburgweg 5, 53115 Bonn, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18338154

Citation

Naz, Ali Ahmad, et al. "AB-QTL Analysis in Winter Wheat: II. Genetic Analysis of Seedling and Field Resistance Against Leaf Rust in a Wheat Advanced Backcross Population." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 116, no. 8, 2008, pp. 1095-104.
Naz AA, Kunert A, Lind V, et al. AB-QTL analysis in winter wheat: II. Genetic analysis of seedling and field resistance against leaf rust in a wheat advanced backcross population. Theor Appl Genet. 2008;116(8):1095-104.
Naz, A. A., Kunert, A., Lind, V., Pillen, K., & Léon, J. (2008). AB-QTL analysis in winter wheat: II. Genetic analysis of seedling and field resistance against leaf rust in a wheat advanced backcross population. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 116(8), 1095-104. https://doi.org/10.1007/s00122-008-0738-y
Naz AA, et al. AB-QTL Analysis in Winter Wheat: II. Genetic Analysis of Seedling and Field Resistance Against Leaf Rust in a Wheat Advanced Backcross Population. Theor Appl Genet. 2008;116(8):1095-104. PubMed PMID: 18338154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - AB-QTL analysis in winter wheat: II. Genetic analysis of seedling and field resistance against leaf rust in a wheat advanced backcross population. AU - Naz,Ali Ahmad, AU - Kunert,Antje, AU - Lind,Volker, AU - Pillen,Klaus, AU - Léon,Jens, PY - 2007/07/26/received PY - 2008/02/19/accepted PY - 2008/3/14/pubmed PY - 2009/11/7/medline PY - 2008/3/14/entrez SP - 1095 EP - 104 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 116 IS - 8 N2 - The present study aimed to localize exotic quantitative trait locus (QTL) alleles for the improvement of leaf rust (P. triticina) resistance in an advanced backcross (AB) population, B22, which is derived from a cross between the winter wheat cultivar Batis (Triticum aestivum) and the synthetic wheat accession Syn022L. The latter was developed from hybridization of T. turgidum ssp. dicoccoides and T. tauschii. Altogether, 250 BC2F3 lines of B22 were assessed for seedling resistance against the leaf rust isolate 77WxR under controlled conditions. In addition, field resistance against leaf rust was evaluated by assessing symptom severity under natural infestation across multiple environments. Simultaneously, population B22 was genotyped with a total of 97 SSR markers, distributed over the wheat A, B and D genomes. The phenotype and genotype data were subjected to QTL analysis by applying a 3-factorial mixed model analysis of variance including the marker genotype as a fixed effect and the environments, the lines and the marker by environment interactions as random effects. The QTL analysis revealed six putative QTLs for seedling resistance and seven for field resistance. For seedling resistance, the effects of exotic QTL alleles improved resistance at all detected loci. The maximum decrease of disease symptoms (-46.3%) was associated with marker locus Xbarc149 on chromosome 1D. For field resistance, two loci had stable main effects across environments and five loci exhibited marker by environment interaction effects. The strongest effects were detected at marker locus Xbarc149 on chromosome 1D, at which the exotic allele decreased seedling symptoms by 46.3% and field symptoms by 43.6%, respectively. Some of the detected QTLs co-localized with known resistance genes, while others appear to be as novel resistance loci. Our findings indicate, that the exotic wheat accession Syn022L may be useful for the improvement of leaf rust resistance in cultivated wheat. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/18338154/AB_QTL_analysis_in_winter_wheat:_II__Genetic_analysis_of_seedling_and_field_resistance_against_leaf_rust_in_a_wheat_advanced_backcross_population_ L2 - https://dx.doi.org/10.1007/s00122-008-0738-y DB - PRIME DP - Unbound Medicine ER -