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[Preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography with macroporous resin].
Zhongguo Zhong Yao Za Zhi. 2007 Dec; 32(24):2595-7.ZZ

Abstract

OBJECTIVE

To develop a method for the preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography (HSCCC).

METHOD

The crude alcohol extracts were eluted on a macroporous resin column and then purified by high speed counter-current chromatography (HSCCC). A two-phase solvent system composed of ethyl acetate: n-butanol: water (2 : 1 : 3) was used, and the lower phase was used as the mobile phase at a flow rate of 1.5 mL x min(-1), while the apparatus rotated at 800 r x min(-1) and the eluate was detected at 254 nm.

RESULT

136 mg gentiopicrin with purity of 99.6% determined by HPLC were obtained from 300 mg crude extraction only in one-step separation and less than 200 minutes.

CONCLUSION

The established method is simple, high efficiency and suitable for large-scale separation of gentiopicrin.

Authors+Show Affiliations

Xu YW
College of Pharmacy, Dalian Medical University, Dalian 116027, China.
Qi Y
No affiliation info available
Han X
No affiliation info available
Xu LN
No affiliation info available
Xu QW
No affiliation info available
Peng JY
No affiliation info available

MeSH

Chromatography, High Pressure LiquidCountercurrent DistributionGentianaGlucosidesIridoid GlucosidesIridoidsPlant RootsPlants, MedicinalResins, SyntheticRhizome

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

18338594

Citation

Xu, You-Wei, et al. "[Preparative Separation of Gentiopicrin From Radix Gentianae By High-speed Counter-current Chromatography With Macroporous Resin]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 32, no. 24, 2007, pp. 2595-7.
Xu YW, Qi Y, Han X, et al. [Preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography with macroporous resin]. Zhongguo Zhong Yao Za Zhi. 2007;32(24):2595-7.
Xu, Y. W., Qi, Y., Han, X., Xu, L. N., Xu, Q. W., & Peng, J. Y. (2007). [Preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography with macroporous resin]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 32(24), 2595-7.
Xu YW, et al. [Preparative Separation of Gentiopicrin From Radix Gentianae By High-speed Counter-current Chromatography With Macroporous Resin]. Zhongguo Zhong Yao Za Zhi. 2007;32(24):2595-7. PubMed PMID: 18338594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography with macroporous resin]. AU - Xu,You-Wei, AU - Qi,Yan, AU - Han,Xu, AU - Xu,Li-Na, AU - Xu,Qi-Wei, AU - Peng,Jin-Yong, PY - 2008/3/15/pubmed PY - 2009/6/2/medline PY - 2008/3/15/entrez SP - 2595 EP - 7 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 32 IS - 24 N2 - OBJECTIVE: To develop a method for the preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography (HSCCC). METHOD: The crude alcohol extracts were eluted on a macroporous resin column and then purified by high speed counter-current chromatography (HSCCC). A two-phase solvent system composed of ethyl acetate: n-butanol: water (2 : 1 : 3) was used, and the lower phase was used as the mobile phase at a flow rate of 1.5 mL x min(-1), while the apparatus rotated at 800 r x min(-1) and the eluate was detected at 254 nm. RESULT: 136 mg gentiopicrin with purity of 99.6% determined by HPLC were obtained from 300 mg crude extraction only in one-step separation and less than 200 minutes. CONCLUSION: The established method is simple, high efficiency and suitable for large-scale separation of gentiopicrin. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/18338594/[Preparative_separation_of_gentiopicrin_from_Radix_Gentianae_by_high_speed_counter_current_chromatography_with_macroporous_resin]_ DB - PRIME DP - Unbound Medicine ER -