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Tissue factor and monocyte chemoattractant protein-1 expression in hypertensive individuals with normal or increased carotid intima-media wall thickness.
Clin Chem 2008; 54(5):814-23CC

Abstract

BACKGROUND

People with hypertension display an inflammatory pattern that includes increased plasma concentrations of monocyte chemoattractant protein 1 (MCP-1) and C-reactive protein (CRP) and enhanced expression of tissue factor (TF) mRNA in blood monocytes.

METHODS

In this study, we investigated the relationship between CRP concentrations and TF and MCP-1 mRNA expression in unstimulated and lipopolysaccharide (LPS)-stimulated monocytes isolated from hypertensives with or without an increase in carotid intima-media thickness (IMT). We also investigated the expression of TF and MCP-1 mRNA and MCP-1 protein after in vitro addition of CRP to monocytes. We measured CRP (by immunonephelometry) and monocyte expression of TF and MCP-1 (by real-time PCR) in 80 untreated hypertensive patients without clinical cardiovascular disease (CVD) or additional risk factors for CVD compared with 41 controls. Based on IMT measured by carotid Doppler ultrasonography, patients were classified into the categories of normal (< or =1 mm) or abnormal (>1 mm). TF and MCP-1 mRNA and MCP-1 protein (by Western blotting) were measured after in vitro addition of CRP to monocytes from 10 randomized controls as well as 10 hypertensives with IMT < or =1 mm and 10 with IMT >1 mm.

RESULTS

CRP and TF and MCP-1 mRNA concentrations were significantly higher in IMT >1 mm hypertensives vs those with IMT < or =1 mm and controls. CRP had no effect on monocyte TF mRNA from either hypertensives or controls. CRP-stimulated monocytes from hypertensives, however, showed increased MCP-1 mRNA and protein expression compared with controls and LPS-stimulated cells.

CONCLUSIONS

Our findings suggest that the inflammatory response of blood monocytes plays an important role in the development of atherosclerosis and hypertension.

Authors+Show Affiliations

Department of Internal Medicine, University of Messina, Messina, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18339698

Citation

Sardo, Maria A., et al. "Tissue Factor and Monocyte Chemoattractant Protein-1 Expression in Hypertensive Individuals With Normal or Increased Carotid Intima-media Wall Thickness." Clinical Chemistry, vol. 54, no. 5, 2008, pp. 814-23.
Sardo MA, Campo S, Mandraffino G, et al. Tissue factor and monocyte chemoattractant protein-1 expression in hypertensive individuals with normal or increased carotid intima-media wall thickness. Clin Chem. 2008;54(5):814-23.
Sardo, M. A., Campo, S., Mandraffino, G., Saitta, C., Bonaiuto, A., Castaldo, M., ... Saitta, A. (2008). Tissue factor and monocyte chemoattractant protein-1 expression in hypertensive individuals with normal or increased carotid intima-media wall thickness. Clinical Chemistry, 54(5), pp. 814-23. doi:10.1373/clinchem.2007.095547.
Sardo MA, et al. Tissue Factor and Monocyte Chemoattractant Protein-1 Expression in Hypertensive Individuals With Normal or Increased Carotid Intima-media Wall Thickness. Clin Chem. 2008;54(5):814-23. PubMed PMID: 18339698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue factor and monocyte chemoattractant protein-1 expression in hypertensive individuals with normal or increased carotid intima-media wall thickness. AU - Sardo,Maria A, AU - Campo,Salvatore, AU - Mandraffino,Giuseppe, AU - Saitta,Carlo, AU - Bonaiuto,Antonio, AU - Castaldo,Maria, AU - Cinquegrani,Maurizio, AU - Pizzimenti,Giovanni, AU - Saitta,Antonino, Y1 - 2008/03/13/ PY - 2008/3/15/pubmed PY - 2008/6/17/medline PY - 2008/3/15/entrez SP - 814 EP - 23 JF - Clinical chemistry JO - Clin. Chem. VL - 54 IS - 5 N2 - BACKGROUND: People with hypertension display an inflammatory pattern that includes increased plasma concentrations of monocyte chemoattractant protein 1 (MCP-1) and C-reactive protein (CRP) and enhanced expression of tissue factor (TF) mRNA in blood monocytes. METHODS: In this study, we investigated the relationship between CRP concentrations and TF and MCP-1 mRNA expression in unstimulated and lipopolysaccharide (LPS)-stimulated monocytes isolated from hypertensives with or without an increase in carotid intima-media thickness (IMT). We also investigated the expression of TF and MCP-1 mRNA and MCP-1 protein after in vitro addition of CRP to monocytes. We measured CRP (by immunonephelometry) and monocyte expression of TF and MCP-1 (by real-time PCR) in 80 untreated hypertensive patients without clinical cardiovascular disease (CVD) or additional risk factors for CVD compared with 41 controls. Based on IMT measured by carotid Doppler ultrasonography, patients were classified into the categories of normal (< or =1 mm) or abnormal (>1 mm). TF and MCP-1 mRNA and MCP-1 protein (by Western blotting) were measured after in vitro addition of CRP to monocytes from 10 randomized controls as well as 10 hypertensives with IMT < or =1 mm and 10 with IMT >1 mm. RESULTS: CRP and TF and MCP-1 mRNA concentrations were significantly higher in IMT >1 mm hypertensives vs those with IMT < or =1 mm and controls. CRP had no effect on monocyte TF mRNA from either hypertensives or controls. CRP-stimulated monocytes from hypertensives, however, showed increased MCP-1 mRNA and protein expression compared with controls and LPS-stimulated cells. CONCLUSIONS: Our findings suggest that the inflammatory response of blood monocytes plays an important role in the development of atherosclerosis and hypertension. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/18339698/Tissue_factor_and_monocyte_chemoattractant_protein_1_expression_in_hypertensive_individuals_with_normal_or_increased_carotid_intima_media_wall_thickness_ L2 - http://www.clinchem.org/cgi/pmidlookup?view=long&amp;pmid=18339698 DB - PRIME DP - Unbound Medicine ER -