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Immunolocalization of aquaporins 1, 2 and 7 in rete testis, efferent ducts, epididymis and vas deferens of adult dog.
Cell Tissue Res. 2008 May; 332(2):329-35.CT

Abstract

The transepithelial movement of water into the male reproductive tract is an essential process for normal male fertility. Protein water channels, referred to as aquaporins (AQPs), are involved in increasing the osmotic permeability of membranes. This study has examined the expression of AQP1, AQP2, and AQP7 in epithelial cells in adult dog efferent ducts, epididymis, and vas deferens. Samples of dog male reproductive tract comprising fragments of the testis, initial segment, caput, corpus and cauda epididymidis, and vas deferens were investigated by immunohistochemistry and Western blotting procedures to show the localization and distribution of the AQPs. AQP1 was noted in rete testis, in efferent ducts, and in vessels in the intertubular space, suggesting that AQP1 participated in the absorption of the large amount of testicular fluid occurring characteristically in the efferent ducts. AQP2 expression was found in the rete testis, efferent ducts and epididymis, whereas AQP7 was expressed in the epithelium of the proximal regions of the epididymis and in the vas deferens. This is the first time that AQP2 and AQP7 have been observed in these regions of mammalian excurrent ducts, but their functional role in the dog male reproductive tract remains unknown. Investigations of AQP biology could be relevant for clinical studies of the male reproductive tract and to technologies for assisted procreation.

Authors+Show Affiliations

Department of Cell Biology, Institute of Biology, UNICAMP, Campinas, SP, Brazil. rdomeniconi@ibb.unesp.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18340467

Citation

Domeniconi, Raquel Fantin, et al. "Immunolocalization of Aquaporins 1, 2 and 7 in Rete Testis, Efferent Ducts, Epididymis and Vas Deferens of Adult Dog." Cell and Tissue Research, vol. 332, no. 2, 2008, pp. 329-35.
Domeniconi RF, Orsi AM, Justulin LA, et al. Immunolocalization of aquaporins 1, 2 and 7 in rete testis, efferent ducts, epididymis and vas deferens of adult dog. Cell Tissue Res. 2008;332(2):329-35.
Domeniconi, R. F., Orsi, A. M., Justulin, L. A., Leme Beu, C. C., & Felisbino, S. L. (2008). Immunolocalization of aquaporins 1, 2 and 7 in rete testis, efferent ducts, epididymis and vas deferens of adult dog. Cell and Tissue Research, 332(2), 329-35. https://doi.org/10.1007/s00441-008-0592-x
Domeniconi RF, et al. Immunolocalization of Aquaporins 1, 2 and 7 in Rete Testis, Efferent Ducts, Epididymis and Vas Deferens of Adult Dog. Cell Tissue Res. 2008;332(2):329-35. PubMed PMID: 18340467.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunolocalization of aquaporins 1, 2 and 7 in rete testis, efferent ducts, epididymis and vas deferens of adult dog. AU - Domeniconi,Raquel Fantin, AU - Orsi,Antonio Marcos, AU - Justulin,Luis Antonio,Jr AU - Leme Beu,Célia Cristina, AU - Felisbino,Sérgio Luis, Y1 - 2008/03/14/ PY - 2007/08/02/received PY - 2008/01/28/accepted PY - 2008/3/15/pubmed PY - 2008/8/30/medline PY - 2008/3/15/entrez SP - 329 EP - 35 JF - Cell and tissue research JO - Cell Tissue Res VL - 332 IS - 2 N2 - The transepithelial movement of water into the male reproductive tract is an essential process for normal male fertility. Protein water channels, referred to as aquaporins (AQPs), are involved in increasing the osmotic permeability of membranes. This study has examined the expression of AQP1, AQP2, and AQP7 in epithelial cells in adult dog efferent ducts, epididymis, and vas deferens. Samples of dog male reproductive tract comprising fragments of the testis, initial segment, caput, corpus and cauda epididymidis, and vas deferens were investigated by immunohistochemistry and Western blotting procedures to show the localization and distribution of the AQPs. AQP1 was noted in rete testis, in efferent ducts, and in vessels in the intertubular space, suggesting that AQP1 participated in the absorption of the large amount of testicular fluid occurring characteristically in the efferent ducts. AQP2 expression was found in the rete testis, efferent ducts and epididymis, whereas AQP7 was expressed in the epithelium of the proximal regions of the epididymis and in the vas deferens. This is the first time that AQP2 and AQP7 have been observed in these regions of mammalian excurrent ducts, but their functional role in the dog male reproductive tract remains unknown. Investigations of AQP biology could be relevant for clinical studies of the male reproductive tract and to technologies for assisted procreation. SN - 0302-766X UR - https://www.unboundmedicine.com/medline/citation/18340467/Immunolocalization_of_aquaporins_1_2_and_7_in_rete_testis_efferent_ducts_epididymis_and_vas_deferens_of_adult_dog_ L2 - https://dx.doi.org/10.1007/s00441-008-0592-x DB - PRIME DP - Unbound Medicine ER -