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In vitro effectiveness of different chemical agents on scolices of hydatid cyst.
J Invest Surg 2008 Mar-Apr; 21(2):71-5JI

Abstract

In the surgical history of hepatic hydatid disease, multiple scolicidal agents have been used for sterilization of the cyst contents. However, none of these agents can be safely used, because most have unacceptable side effects, such as toxicity, caustic sclerosing cholangitis, bile duct stricture, and hypernatremia. Protoscolices were aseptically removed from liver hydatid cysts obtained from sheep slaughtered at the municipal abattoir in Kahramanmaras, Turkey. In this study, the effects of 0.9% NaCl (control group), 20% NaCl (hypertonic saline), 20% silver nitrate, albendazole 20 mg/cm3, 50% dextrose (hypertonic glucose), and 20% mannitol and aminomix-1 solutions on echinococe cysts were investigated under in vitro conditions. After 5, 10, 15, 20, 25, 30, 45, and 60 min for each concentration, the first 100 protoscolices were counted on the 10x microscopical field. Protoscolices, which showed positive staining by eosine were considered as dead ones. The averages of dead and total protoscolices were calculated. Our results showed that all observed protoscolices were dead after the treatments by 20% silver nitrate in 20 min, by 50% dextrose and by aminomix-1 solution in 30 min, and by 20% NaCl and by 20% mannitol in 45 min. Albendazole at 20 mg/cm3 was observed to lead to death of 65% of protoscolices in the first 5 min and 70% of protoscolices at the end of 60 min. Compared with 0.9% NaCl (saline), all of these scolicidal agents were significantly effective (p < .05). Aminomix-1, 20% mannitol, and 50% dextrose solutions may be used in percutaneous and surgical treatment of hydatid cyst. They may be preferred because they are readily available, can be administered intravenously, and have an equal or greater effectiveness than 20% hypertonic saline.

Authors+Show Affiliations

Department of General Surgery, Faculty of Medicine, Kahramanmaras Sutcuimam University, Kahramanmaras, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

18340623

Citation

Caglar, Recep, et al. "In Vitro Effectiveness of Different Chemical Agents On Scolices of Hydatid Cyst." Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research, vol. 21, no. 2, 2008, pp. 71-5.
Caglar R, Yuzbasioglu MF, Bulbuloglu E, et al. In vitro effectiveness of different chemical agents on scolices of hydatid cyst. J Invest Surg. 2008;21(2):71-5.
Caglar, R., Yuzbasioglu, M. F., Bulbuloglu, E., Gul, M., Ezberci, F., & Kale, I. T. (2008). In vitro effectiveness of different chemical agents on scolices of hydatid cyst. Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research, 21(2), pp. 71-5. doi:10.1080/08941930701883640.
Caglar R, et al. In Vitro Effectiveness of Different Chemical Agents On Scolices of Hydatid Cyst. J Invest Surg. 2008;21(2):71-5. PubMed PMID: 18340623.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro effectiveness of different chemical agents on scolices of hydatid cyst. AU - Caglar,Recep, AU - Yuzbasioglu,Mehmet Fatih, AU - Bulbuloglu,Ertan, AU - Gul,Mustafa, AU - Ezberci,Fikret, AU - Kale,Ilhami Taner, PY - 2008/3/15/pubmed PY - 2008/6/27/medline PY - 2008/3/15/entrez SP - 71 EP - 5 JF - Journal of investigative surgery : the official journal of the Academy of Surgical Research JO - J Invest Surg VL - 21 IS - 2 N2 - In the surgical history of hepatic hydatid disease, multiple scolicidal agents have been used for sterilization of the cyst contents. However, none of these agents can be safely used, because most have unacceptable side effects, such as toxicity, caustic sclerosing cholangitis, bile duct stricture, and hypernatremia. Protoscolices were aseptically removed from liver hydatid cysts obtained from sheep slaughtered at the municipal abattoir in Kahramanmaras, Turkey. In this study, the effects of 0.9% NaCl (control group), 20% NaCl (hypertonic saline), 20% silver nitrate, albendazole 20 mg/cm3, 50% dextrose (hypertonic glucose), and 20% mannitol and aminomix-1 solutions on echinococe cysts were investigated under in vitro conditions. After 5, 10, 15, 20, 25, 30, 45, and 60 min for each concentration, the first 100 protoscolices were counted on the 10x microscopical field. Protoscolices, which showed positive staining by eosine were considered as dead ones. The averages of dead and total protoscolices were calculated. Our results showed that all observed protoscolices were dead after the treatments by 20% silver nitrate in 20 min, by 50% dextrose and by aminomix-1 solution in 30 min, and by 20% NaCl and by 20% mannitol in 45 min. Albendazole at 20 mg/cm3 was observed to lead to death of 65% of protoscolices in the first 5 min and 70% of protoscolices at the end of 60 min. Compared with 0.9% NaCl (saline), all of these scolicidal agents were significantly effective (p < .05). Aminomix-1, 20% mannitol, and 50% dextrose solutions may be used in percutaneous and surgical treatment of hydatid cyst. They may be preferred because they are readily available, can be administered intravenously, and have an equal or greater effectiveness than 20% hypertonic saline. SN - 0894-1939 UR - https://www.unboundmedicine.com/medline/citation/18340623/In_vitro_effectiveness_of_different_chemical_agents_on_scolices_of_hydatid_cyst_ L2 - http://www.tandfonline.com/doi/full/10.1080/08941930701883640 DB - PRIME DP - Unbound Medicine ER -