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Advanced glycosylated end products-mediated activation of polymorphonuclear neutrophils in diabetes mellitus and associated oxidative stress.
Indian J Biochem Biophys. 2007 Oct; 44(5):373-8.IJ

Abstract

Two important consequences of hyperglycemia in diabetes are development of oxidative stress and formation of advanced glycation end products (AGE) which are known to be associated with diabetic complications. Relationship between AGE formation and development of oxidative stress (OS) is yet to be established. In the present study, the involvement of AGE in PMN-mediated ROS generation and the associated OS were investigated in type 2 diabetic mellitus (DM) patients. We assessed OS parameters (serum MDA, FRAP and GSH), PMN oxidative functions (respiratory burst and superoxide production) and total serum AGE in 90 subjects divided equally in three groups--control group, Group I consisting of type 2 diabetic patients without microvascular complications and Group II consisting of type 2 diabetic patients with microvascular complications. PMNs isolated from both groups (I and II) exhibited higher level of respiratory burst (RB) and produced increased amount of superoxide anion as compared to the controls. The increase was more pronounced in diabetes with complications, as compared to those without. Serum malondialdehyde (MDA) level was elevated, whereas glutathione (GSH) and ferric reducing ability of plasma (FRAP) levels were significantly reduced in diabetes as compared to the controls, suggesting the presence of oxidative stress in DM. A positive correlation between PMN oxidative function and OS parameters suggested the involvement of PMN in the development of OS in DM. Serum AGE level was also elevated in diabetic groups as compared to the controls. Further, the positive correlation between serum AGE level and PMN oxidative function suggested the involvement of AGE in increased RB and generation of reactive oxygen species (ROS) by resting diabetic PMN. The results of the study indicate that AGE-PMN interaction possibly upregulates NADPH oxidase, leading to enhanced ROS generation and thus contributes to the pathogenesis in diabetes.

Authors+Show Affiliations

Department of Biochemistry, University College of Medical Sciences and G.T.B. Hospital (University of Delhi), Dilshad Garden, Delhi-110 095, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18341213

Citation

Gupta, Anjali, et al. "Advanced Glycosylated End Products-mediated Activation of Polymorphonuclear Neutrophils in Diabetes Mellitus and Associated Oxidative Stress." Indian Journal of Biochemistry & Biophysics, vol. 44, no. 5, 2007, pp. 373-8.
Gupta A, Tripathi AK, Tripathi RL, et al. Advanced glycosylated end products-mediated activation of polymorphonuclear neutrophils in diabetes mellitus and associated oxidative stress. Indian J Biochem Biophys. 2007;44(5):373-8.
Gupta, A., Tripathi, A. K., Tripathi, R. L., Madhu, S. V., & Banerjee, B. D. (2007). Advanced glycosylated end products-mediated activation of polymorphonuclear neutrophils in diabetes mellitus and associated oxidative stress. Indian Journal of Biochemistry & Biophysics, 44(5), 373-8.
Gupta A, et al. Advanced Glycosylated End Products-mediated Activation of Polymorphonuclear Neutrophils in Diabetes Mellitus and Associated Oxidative Stress. Indian J Biochem Biophys. 2007;44(5):373-8. PubMed PMID: 18341213.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Advanced glycosylated end products-mediated activation of polymorphonuclear neutrophils in diabetes mellitus and associated oxidative stress. AU - Gupta,Anjali, AU - Tripathi,A K, AU - Tripathi,R L, AU - Madhu,S V, AU - Banerjee,B D, PY - 2008/3/18/pubmed PY - 2008/4/9/medline PY - 2008/3/18/entrez SP - 373 EP - 8 JF - Indian journal of biochemistry & biophysics JO - Indian J Biochem Biophys VL - 44 IS - 5 N2 - Two important consequences of hyperglycemia in diabetes are development of oxidative stress and formation of advanced glycation end products (AGE) which are known to be associated with diabetic complications. Relationship between AGE formation and development of oxidative stress (OS) is yet to be established. In the present study, the involvement of AGE in PMN-mediated ROS generation and the associated OS were investigated in type 2 diabetic mellitus (DM) patients. We assessed OS parameters (serum MDA, FRAP and GSH), PMN oxidative functions (respiratory burst and superoxide production) and total serum AGE in 90 subjects divided equally in three groups--control group, Group I consisting of type 2 diabetic patients without microvascular complications and Group II consisting of type 2 diabetic patients with microvascular complications. PMNs isolated from both groups (I and II) exhibited higher level of respiratory burst (RB) and produced increased amount of superoxide anion as compared to the controls. The increase was more pronounced in diabetes with complications, as compared to those without. Serum malondialdehyde (MDA) level was elevated, whereas glutathione (GSH) and ferric reducing ability of plasma (FRAP) levels were significantly reduced in diabetes as compared to the controls, suggesting the presence of oxidative stress in DM. A positive correlation between PMN oxidative function and OS parameters suggested the involvement of PMN in the development of OS in DM. Serum AGE level was also elevated in diabetic groups as compared to the controls. Further, the positive correlation between serum AGE level and PMN oxidative function suggested the involvement of AGE in increased RB and generation of reactive oxygen species (ROS) by resting diabetic PMN. The results of the study indicate that AGE-PMN interaction possibly upregulates NADPH oxidase, leading to enhanced ROS generation and thus contributes to the pathogenesis in diabetes. SN - 0301-1208 UR - https://www.unboundmedicine.com/medline/citation/18341213/Advanced_glycosylated_end_products_mediated_activation_of_polymorphonuclear_neutrophils_in_diabetes_mellitus_and_associated_oxidative_stress_ DB - PRIME DP - Unbound Medicine ER -