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Raman multiplexers for alternative gene splicing.
Anal Chem. 2008 May 01; 80(9):3342-9.AC

Abstract

Nonfluorescent labels were used for an array-format multiplex detection of alternative splice junctions of breast cancer susceptibility gene 1 (BRCA1) by surface-enhanced Raman scattering (SERS). A four-plex detection scheme using nonfluorescent labels was demonstrated for DNA sequences specific to four BRCA1 alternative splice variants: Delta(11q) (the last 3309 nt deleted from exon 11), Delta(9, 10) (exon 9 and 10 deleted), Delta(5) (exon 5 deleted), and Delta(5q, 6) (the last 22 nt of exon 5 and the entire exon 6 is deleted). This is the first proof-of-concept study to apply SERS-based detection using nonfluorescent labels to investigate alternative gene splicing. Detection sensitivity of up to 1 fM was demonstrated for the Raman labels chosen to clearly discriminate the splice junctions via specific target identification. The proposed approach has the potential to become a highly sensitive and selective tool for comprehensive alternative splicing profiling of BRCA1 or other genes relevant to specific diseases.

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Authors+Show Affiliations

Sun L
Department of Agricultural and Biological Engineering and Bindley Bioscience Center, Purdue University, 225 South University Street, West Lafayette, Indiana 47907, USA.
Yu C
No affiliation info available
Irudayaraj J
No affiliation info available

MeSH

Alternative SplicingBase SequenceDNADNA ProbesGenes, BRCA1GoldHumansMolecular Sequence DataOligonucleotidesSensitivity and SpecificitySpectrum Analysis, Raman

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18341356

Citation

Sun, Lan, et al. "Raman Multiplexers for Alternative Gene Splicing." Analytical Chemistry, vol. 80, no. 9, 2008, pp. 3342-9.
Sun L, Yu C, Irudayaraj J. Raman multiplexers for alternative gene splicing. Anal Chem. 2008;80(9):3342-9.
Sun, L., Yu, C., & Irudayaraj, J. (2008). Raman multiplexers for alternative gene splicing. Analytical Chemistry, 80(9), 3342-9. https://doi.org/10.1021/ac702542n
Sun L, Yu C, Irudayaraj J. Raman Multiplexers for Alternative Gene Splicing. Anal Chem. 2008 May 1;80(9):3342-9. PubMed PMID: 18341356.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Raman multiplexers for alternative gene splicing. AU - Sun,Lan, AU - Yu,Chenxu, AU - Irudayaraj,Joseph, Y1 - 2008/03/15/ PY - 2008/3/18/pubmed PY - 2008/5/21/medline PY - 2008/3/18/entrez SP - 3342 EP - 9 JF - Analytical chemistry JO - Anal Chem VL - 80 IS - 9 N2 - Nonfluorescent labels were used for an array-format multiplex detection of alternative splice junctions of breast cancer susceptibility gene 1 (BRCA1) by surface-enhanced Raman scattering (SERS). A four-plex detection scheme using nonfluorescent labels was demonstrated for DNA sequences specific to four BRCA1 alternative splice variants: Delta(11q) (the last 3309 nt deleted from exon 11), Delta(9, 10) (exon 9 and 10 deleted), Delta(5) (exon 5 deleted), and Delta(5q, 6) (the last 22 nt of exon 5 and the entire exon 6 is deleted). This is the first proof-of-concept study to apply SERS-based detection using nonfluorescent labels to investigate alternative gene splicing. Detection sensitivity of up to 1 fM was demonstrated for the Raman labels chosen to clearly discriminate the splice junctions via specific target identification. The proposed approach has the potential to become a highly sensitive and selective tool for comprehensive alternative splicing profiling of BRCA1 or other genes relevant to specific diseases. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/18341356/Raman_multiplexers_for_alternative_gene_splicing_ L2 - https://doi.org/10.1021/ac702542n DB - PRIME DP - Unbound Medicine ER -