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Raman multiplexers for alternative gene splicing.
Anal Chem. 2008 May 01; 80(9):3342-9.AC

Abstract

Nonfluorescent labels were used for an array-format multiplex detection of alternative splice junctions of breast cancer susceptibility gene 1 (BRCA1) by surface-enhanced Raman scattering (SERS). A four-plex detection scheme using nonfluorescent labels was demonstrated for DNA sequences specific to four BRCA1 alternative splice variants: Delta(11q) (the last 3309 nt deleted from exon 11), Delta(9, 10) (exon 9 and 10 deleted), Delta(5) (exon 5 deleted), and Delta(5q, 6) (the last 22 nt of exon 5 and the entire exon 6 is deleted). This is the first proof-of-concept study to apply SERS-based detection using nonfluorescent labels to investigate alternative gene splicing. Detection sensitivity of up to 1 fM was demonstrated for the Raman labels chosen to clearly discriminate the splice junctions via specific target identification. The proposed approach has the potential to become a highly sensitive and selective tool for comprehensive alternative splicing profiling of BRCA1 or other genes relevant to specific diseases.

Authors+Show Affiliations

Department of Agricultural and Biological Engineering and Bindley Bioscience Center, Purdue University, 225 South University Street, West Lafayette, Indiana 47907, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18341356

Citation

Sun, Lan, et al. "Raman Multiplexers for Alternative Gene Splicing." Analytical Chemistry, vol. 80, no. 9, 2008, pp. 3342-9.
Sun L, Yu C, Irudayaraj J. Raman multiplexers for alternative gene splicing. Anal Chem. 2008;80(9):3342-9.
Sun, L., Yu, C., & Irudayaraj, J. (2008). Raman multiplexers for alternative gene splicing. Analytical Chemistry, 80(9), 3342-9. https://doi.org/10.1021/ac702542n
Sun L, Yu C, Irudayaraj J. Raman Multiplexers for Alternative Gene Splicing. Anal Chem. 2008 May 1;80(9):3342-9. PubMed PMID: 18341356.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Raman multiplexers for alternative gene splicing. AU - Sun,Lan, AU - Yu,Chenxu, AU - Irudayaraj,Joseph, Y1 - 2008/03/15/ PY - 2008/3/18/pubmed PY - 2008/5/21/medline PY - 2008/3/18/entrez SP - 3342 EP - 9 JF - Analytical chemistry JO - Anal Chem VL - 80 IS - 9 N2 - Nonfluorescent labels were used for an array-format multiplex detection of alternative splice junctions of breast cancer susceptibility gene 1 (BRCA1) by surface-enhanced Raman scattering (SERS). A four-plex detection scheme using nonfluorescent labels was demonstrated for DNA sequences specific to four BRCA1 alternative splice variants: Delta(11q) (the last 3309 nt deleted from exon 11), Delta(9, 10) (exon 9 and 10 deleted), Delta(5) (exon 5 deleted), and Delta(5q, 6) (the last 22 nt of exon 5 and the entire exon 6 is deleted). This is the first proof-of-concept study to apply SERS-based detection using nonfluorescent labels to investigate alternative gene splicing. Detection sensitivity of up to 1 fM was demonstrated for the Raman labels chosen to clearly discriminate the splice junctions via specific target identification. The proposed approach has the potential to become a highly sensitive and selective tool for comprehensive alternative splicing profiling of BRCA1 or other genes relevant to specific diseases. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/18341356/Raman_multiplexers_for_alternative_gene_splicing_ L2 - https://doi.org/10.1021/ac702542n DB - PRIME DP - Unbound Medicine ER -