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Capillary electrophoresis of aminoglycosides with argon-ion laser-induced fluorescence detection.
J Chromatogr A. 2008 Apr 25; 1188(2):331-3.JC

Abstract

A new analytical method for aminoglycosides (kanamycin, bekanamycin, paromomycin and tobramycin) based on capillary electrophoretic separation and argon-ion laser-induced fluorescence detection has been developed. 6-Carboxyfluorescein succinimidyl ester (CFSE) was used for precolumn derivatization of the non-fluorescent aminoglycosides. Optimal separation and detection were obtained with an electrophoretic buffer of 30 mM sodium borate (pH 9.0) and an air-cooled argon-ion laser (excitation at 488 nm, emission at 520 nm). The concentration limits of detection in aqueous solution were 3.9-8.2 nM. Combined with a simple cleanup procedure, this method can be applied to the determination of aminoglycosides in human plasma. A calibration curve ranging from 0.15 to 30 microM was shown to be linear. The limits of detection of aminoglycosides in human plasma were between 14.4 and 24.0 nM. Recoveries of spiked aminoglycosides in human plasma were between 92 and 105%.

Authors+Show Affiliations

Department of Applied Chemistry, Chaoyang University of Technology, Wufong, Taichung County 413, Taiwan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18343388

Citation

Lin, Yi-Fang, et al. "Capillary Electrophoresis of Aminoglycosides With Argon-ion Laser-induced Fluorescence Detection." Journal of Chromatography. A, vol. 1188, no. 2, 2008, pp. 331-3.
Lin YF, Wang YC, Chang SY. Capillary electrophoresis of aminoglycosides with argon-ion laser-induced fluorescence detection. J Chromatogr A. 2008;1188(2):331-3.
Lin, Y. F., Wang, Y. C., & Chang, S. Y. (2008). Capillary electrophoresis of aminoglycosides with argon-ion laser-induced fluorescence detection. Journal of Chromatography. A, 1188(2), 331-3. https://doi.org/10.1016/j.chroma.2008.01.088
Lin YF, Wang YC, Chang SY. Capillary Electrophoresis of Aminoglycosides With Argon-ion Laser-induced Fluorescence Detection. J Chromatogr A. 2008 Apr 25;1188(2):331-3. PubMed PMID: 18343388.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary electrophoresis of aminoglycosides with argon-ion laser-induced fluorescence detection. AU - Lin,Yi-Fang, AU - Wang,Yung-Chi, AU - Chang,Sarah Y, Y1 - 2008/03/04/ PY - 2007/11/02/received PY - 2008/01/18/revised PY - 2008/01/24/accepted PY - 2008/3/18/pubmed PY - 2008/7/9/medline PY - 2008/3/18/entrez SP - 331 EP - 3 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1188 IS - 2 N2 - A new analytical method for aminoglycosides (kanamycin, bekanamycin, paromomycin and tobramycin) based on capillary electrophoretic separation and argon-ion laser-induced fluorescence detection has been developed. 6-Carboxyfluorescein succinimidyl ester (CFSE) was used for precolumn derivatization of the non-fluorescent aminoglycosides. Optimal separation and detection were obtained with an electrophoretic buffer of 30 mM sodium borate (pH 9.0) and an air-cooled argon-ion laser (excitation at 488 nm, emission at 520 nm). The concentration limits of detection in aqueous solution were 3.9-8.2 nM. Combined with a simple cleanup procedure, this method can be applied to the determination of aminoglycosides in human plasma. A calibration curve ranging from 0.15 to 30 microM was shown to be linear. The limits of detection of aminoglycosides in human plasma were between 14.4 and 24.0 nM. Recoveries of spiked aminoglycosides in human plasma were between 92 and 105%. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18343388/Capillary_electrophoresis_of_aminoglycosides_with_argon_ion_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)00382-8 DB - PRIME DP - Unbound Medicine ER -